Restriction map for
pF1KE1761
(Linear) (Six-base) MAPSORT of: KIEE1761.seq Check: 9756 from: 1 to: 804
KIEE1761.orf
With 182 enzymes: SgfI *
October 23, 2018 17:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 804
Size: 421 383
AflIII A'CryG_T
Cuts at: 0 427 804
Size: 427 377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 700 732 804
Size: 700 32 72
Fragments arranged by size:
700 72 32
ApoI r'AATT_y
Cuts at: 0 487 804
Size: 487 317
AvrII C'CTAG_G
Cuts at: 0 183 804
Size: 183 621
BanI G'GyrC_C
Cuts at: 0 586 804
Size: 586 218
BanII G_rGCy'C
Cuts at: 0 239 804
Size: 239 565
BbsI GAAGACnn'nnnn_
Cuts at: 0 409 804
Size: 409 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 233 804
Size: 199 34 571
Fragments arranged by size:
571 199 34
BclI T'GATC_A
Cuts at: 0 95 635 804
Size: 95 540 169
Fragments arranged by size:
540 169 95
BglI GCCn_nnn'nGGC
Cuts at: 0 322 595 804
Size: 322 273 209
Fragments arranged by size:
322 273 209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 804
Size: 347 457
BsaHI Gr'CG_yC
Cuts at: 0 710 804
Size: 710 94
BsaWI w'CCGG_w
Cuts at: 0 302 804
Size: 302 502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 804
Size: 301 503
BseYI C'CCAG_C
Cuts at: 0 317 474 804
Size: 317 157 330
Fragments arranged by size:
330 317 157
Bsp1286I G_dGCh'C
Cuts at: 0 239 804
Size: 239 565
BspEI T'CCGG_A
Cuts at: 0 302 804
Size: 302 502
BspHI T'CATG_A
Cuts at: 0 359 804
Size: 359 445
BstXI CCAn_nnnn'nTGG
Cuts at: 0 325 804
Size: 325 479
DrdI GACnn_nn'nnGTC
Cuts at: 0 340 804
Size: 340 464
EaeI y'GGCC_r
Cuts at: 0 170 443 804
Size: 170 273 361
Fragments arranged by size:
361 273 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 203 804
Size: 203 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 421 804
Size: 347 74 383
Fragments arranged by size:
383 347 74
EcoO109I rG'GnC_Cy
Cuts at: 0 57 69 804
Size: 57 12 735
Fragments arranged by size:
735 57 12
EcoRI G'AATT_C
Cuts at: 0 487 804
Size: 487 317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 804
Size: 495 309
FspI TGC'GCA
Cuts at: 0 178 221 804
Size: 178 43 583
Fragments arranged by size:
583 178 43
FspAI rTGC'GCAy
Cuts at: 0 178 804
Size: 178 626
HaeII r_GCGC'y
Cuts at: 0 203 350 804
Size: 203 147 454
Fragments arranged by size:
454 203 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 804
Size: 80 32 692
Fragments arranged by size:
692 80 32
HincII GTy'rAC
Cuts at: 0 12 804
Size: 12 792
HpaI GTT'AAC
Cuts at: 0 12 804
Size: 12 792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 666 804
Size: 287 379 138
Fragments arranged by size:
379 287 138
MslI CAynn'nnrTG
Cuts at: 0 614 804
Size: 614 190
MspA1I CmG'CkG
Cuts at: 0 101 321 692 789 804
Size: 101 220 371 97 15
Fragments arranged by size:
371 220 101 97 15
NspI r_CATG'y
Cuts at: 0 431 804
Size: 431 373
PciI A'CATG_T
Cuts at: 0 427 804
Size: 427 377
PfoI T'CCnGG_A
Cuts at: 0 773 804
Size: 773 31
PshAI GACnn'nnGTC
Cuts at: 0 428 804
Size: 428 376
PstI C_TGCA'G
Cuts at: 0 788 804
Size: 788 16
PvuII CAG'CTG
Cuts at: 0 321 692 789 804
Size: 321 371 97 15
Fragments arranged by size:
371 321 97 15
ScaI AGT'ACT
Cuts at: 0 496 804
Size: 496 308
SfcI C'TryA_G
Cuts at: 0 784 804
Size: 784 20
StuI AGG'CCT
Cuts at: 0 92 804
Size: 92 712
StyI C'CwwG_G
Cuts at: 0 183 804
Size: 183 621
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 566 804
Size: 52 514 238
Fragments arranged by size:
514 238 52
TatI w'GTAC_w
Cuts at: 0 224 494 804
Size: 224 270 310
Fragments arranged by size:
310 270 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 804
Size: 471 333
Enzymes that do cut:
AcuI AflIII AloI ApoI AvrII BanI BanII BbsI
BcgI BclI BglI BpmI BsaHI BsaWI BseRI BseYI
Bsp1286I BspEI BspHI BstXI DrdI EaeI EciI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HpaI MmeI MslI MspA1I NspI PciI PfoI PshAI
PstI PvuII ScaI SfcI StuI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BbeI BbvCI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EagI
EarI EcoICRI EcoNI EcoRV FseI HindIII KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI