(Linear) (Six-base) MAPSORT of: KIEE1756.seq  Check: 7877  from: 1  to: 783



KIEE1756.orf

 With 182 enzymes: SgfI * 

                        July 3, 2020 20:21  ..

AflIII A'CryG_T

Cuts at:      0     372     783 
   Size:        372     411
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     716     783 
   Size:        716      67
 
AlwNI CAG_nnn'CTG

Cuts at:      0     578     783 
   Size:        578     205
 
ApoI r'AATT_y

Cuts at:      0      65     783 
   Size:         65     718
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     424     783 
   Size:        424     359
 
BclI T'GATC_A

Cuts at:      0     348     783 
   Size:        348     435
 
BmgBI CAC'GTC

Cuts at:      0     761     783 
   Size:        761      22
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     575     783 
   Size:        575     208
 
BsaBI GATnn'nnATC

Cuts at:      0     234     353     783 
   Size:        234     119     430

  Fragments arranged by size: 

                 430     234     119
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     268     322     692     783 
   Size:        268      54     370      91

  Fragments arranged by size: 

                 370     268      91      54
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     304     783 
   Size:        304     479
 
BspHI T'CATG_A

Cuts at:      0     177     194     783 
   Size:        177      17     589

  Fragments arranged by size: 

                 589     177      17
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     124     783 
   Size:        124     659
 
BstEII G'GTnAC_C

Cuts at:      0     456     783 
   Size:        456     327
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     109     672     751     783 
   Size:        109     563      79      32

  Fragments arranged by size: 

                 563     109      79      32
 
BstYI r'GATC_y

Cuts at:      0     229     783 
   Size:        229     554
 
BtgI C'CryG_G

Cuts at:      0     744     783 
   Size:        744      39
 
EaeI y'GGCC_r

Cuts at:      0      38     783 
   Size:         38     745
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     630     783 
   Size:        630     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      38      70     485     517     783 
   Size:         38      32     415      32     266

  Fragments arranged by size: 

                 415     266      38      32      32
 
HindIII A'AGCT_T

Cuts at:      0     545     783 
   Size:        545     238
 
MslI CAynn'nnrTG

Cuts at:      0      58     103     783 
   Size:         58      45     680

  Fragments arranged by size: 

                 680      58      45
 
MspA1I CmG'CkG

Cuts at:      0     480     783 
   Size:        480     303
 
NcoI C'CATG_G

Cuts at:      0     744     783 
   Size:        744      39
 
NspI r_CATG'y

Cuts at:      0     376     783 
   Size:        376     407
 
PciI A'CATG_T

Cuts at:      0     372     783 
   Size:        372     411
 
PvuII CAG'CTG

Cuts at:      0     480     783 
   Size:        480     303
 
SexAI A'CCwGG_T

Cuts at:      0     696     783 
   Size:        696      87
 
StyI C'CwwG_G

Cuts at:      0     744     783 
   Size:        744      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     115     783 
   Size:        115     668
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     742     783 
   Size:        742      41
 

 Enzymes that do cut:

   AflIII     AlfI    AlwNI     ApoI    BciVI     BclI    BmgBI     BplI
    BsaBI    BseRI    BsmBI    BspHI   BstAPI   BstEII    BstXI    BstYI
     BtgI     EaeI     EciI    Hin4I  HindIII     MslI   MspA1I     NcoI
     NspI     PciI    PvuII    SexAI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI