Restriction map for
pF1KE1598
(Linear) (Six-base) MAPSORT of: KIEE1598.seq Check: 5562 from: 1 to: 4296
KIEE1598.orf
With 182 enzymes: SgfI *
August 16, 2010 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 397 4296
Size: 397 3899
AccI GT'mk_AC
Cuts at: 0 904 1916 4296
Size: 904 1012 2380
Fragments arranged by size:
2380 1012 904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2224 3635 4154 4296
Size: 2224 1411 519 142
Fragments arranged by size:
2224 1411 519 142
AflII C'TTAA_G
Cuts at: 0 1345 1426 4296
Size: 1345 81 2870
Fragments arranged by size:
2870 1345 81
AflIII A'CryG_T
Cuts at: 0 4031 4296
Size: 4031 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 725 3515 3859 4296
Size: 725 2790 344 437
Fragments arranged by size:
2790 725 437 344
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1187 1219 4296
Size: 1187 32 3077
Fragments arranged by size:
3077 1187 32
AlwNI CAG_nnn'CTG
Cuts at: 0 567 630 643 2247 4296
Size: 567 63 13 1604 2049
Fragments arranged by size:
2049 1604 567 63 13
ApoI r'AATT_y
Cuts at: 0 142 468 604 675 1208 2284 2441 2926
Size: 142 326 136 71 533 1076 157 485
Cuts at: 2926 3106 4296
Size: 180 1190
Fragments arranged by size:
1190 1076 533 485 326 180 157 142
136 71
AseI AT'TA_AT
Cuts at: 0 2483 4261 4296
Size: 2483 1778 35
Fragments arranged by size:
2483 1778 35
BamHI G'GATC_C
Cuts at: 0 151 4296
Size: 151 4145
BanI G'GyrC_C
Cuts at: 0 2630 3253 4296
Size: 2630 623 1043
Fragments arranged by size:
2630 1043 623
BanII G_rGCy'C
Cuts at: 0 2065 2607 4296
Size: 2065 542 1689
Fragments arranged by size:
2065 1689 542
BbsI GAAGACnn'nnnn_
Cuts at: 0 1142 1498 1814 2141 3733 4296
Size: 1142 356 316 327 1592 563
Fragments arranged by size:
1592 1142 563 356 327 316
BciVI GTATCCnnnnn_n'
Cuts at: 0 1757 2853 4296
Size: 1757 1096 1443
Fragments arranged by size:
1757 1443 1096
BclI T'GATC_A
Cuts at: 0 1053 3065 4296
Size: 1053 2012 1231
Fragments arranged by size:
2012 1231 1053
BglI GCCn_nnn'nGGC
Cuts at: 0 3634 4296
Size: 3634 662
BglII A'GATC_T
Cuts at: 0 4139 4296
Size: 4139 157
BlpI GC'TnA_GC
Cuts at: 0 794 4296
Size: 794 3502
Bme1580I G_kGCm'C
Cuts at: 0 3256 4296
Size: 3256 1040
BmgBI CAC'GTC
Cuts at: 0 2595 4296
Size: 2595 1701
BmrI ACTGGGnnnn_n'
Cuts at: 0 983 2289 4296
Size: 983 1306 2007
Fragments arranged by size:
2007 1306 983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2183 4296
Size: 2183 2113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 1910 1942 3911 4296
Size: 543 1367 32 1969 385
Fragments arranged by size:
1969 1367 543 385 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 1987 3698 4296
Size: 1603 384 1711 598
Fragments arranged by size:
1711 1603 598 384
BsaI GGTCTCn'nnnn_
Cuts at: 0 511 3564 4296
Size: 511 3053 732
Fragments arranged by size:
3053 732 511
BsaAI yAC'GTr
Cuts at: 0 445 1920 2650 4034 4099 4296
Size: 445 1475 730 1384 65 197
Fragments arranged by size:
1475 1384 730 445 197 65
BsaBI GATnn'nnATC
Cuts at: 0 2990 4296
Size: 2990 1306
BsaWI w'CCGG_w
Cuts at: 0 1907 4296
Size: 1907 2389
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1067 1097 1187 1217 4296
Size: 1067 30 90 30 3079
Fragments arranged by size:
3079 1067 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 900 4296
Size: 900 3396
BseYI C'CCAG_C
Cuts at: 0 931 1842 3522 4296
Size: 931 911 1680 774
Fragments arranged by size:
1680 931 911 774
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 82 4296
Size: 11 71 4214
Fragments arranged by size:
4214 71 11
BsiHKAI G_wGCw'C
Cuts at: 0 2523 4296
Size: 2523 1773
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4206 4296
Size: 4206 90
Bsp1286I G_dGCh'C
Cuts at: 0 2065 2523 2607 3256 4296
Size: 2065 458 84 649 1040
Fragments arranged by size:
2065 1040 649 458 84
BspHI T'CATG_A
Cuts at: 0 3851 4296
Size: 3851 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 2807 4296
Size: 397 2410 1489
Fragments arranged by size:
2410 1489 397
BsrDI GCAATG_nn'
Cuts at: 0 1991 2832 4296
Size: 1991 841 1464
Fragments arranged by size:
1991 1464 841
BsrGI T'GTAC_A
Cuts at: 0 511 4296
Size: 511 3785
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2107 4296
Size: 2107 2189
BstBI TT'CG_AA
Cuts at: 0 3235 4296
Size: 3235 1061
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1658 3510 4296
Size: 1658 1852 786
Fragments arranged by size:
1852 1658 786
BstYI r'GATC_y
Cuts at: 0 151 786 2244 2962 4058 4139 4296
Size: 151 635 1458 718 1096 81 157
Fragments arranged by size:
1458 1096 718 635 157 151 81
BstZ17I GTA'TAC
Cuts at: 0 1917 4296
Size: 1917 2379
Bsu36I CC'TnA_GG
Cuts at: 0 2525 4296
Size: 2525 1771
BtgI C'CryG_G
Cuts at: 0 832 2843 4296
Size: 832 2011 1453
Fragments arranged by size:
2011 1453 832
BtsI GCAGTG_nn'
Cuts at: 0 2077 4296
Size: 2077 2219
DraI TTT'AAA
Cuts at: 0 944 1647 4296
Size: 944 703 2649
Fragments arranged by size:
2649 944 703
EaeI y'GGCC_r
Cuts at: 0 253 1585 1632 3560 3776 4296
Size: 253 1332 47 1928 216 520
Fragments arranged by size:
1928 1332 520 253 216 47
EarI CTCTTCn'nnn_
Cuts at: 0 103 1595 1686 3814 4130 4296
Size: 103 1492 91 2128 316 166
Fragments arranged by size:
2128 1492 316 166 103 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 543 1910 1942 2224 3635 3911 4154 4296
Size: 543 1367 32 282 1411 276 243 142
Fragments arranged by size:
1411 1367 543 282 276 243 142 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1041 4296
Size: 1041 3255
EcoRI G'AATT_C
Cuts at: 0 142 4296
Size: 142 4154
EcoRV GAT'ATC
Cuts at: 0 456 4296
Size: 456 3840
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1102 1255 1644 3330 4296
Size: 1102 153 389 1686 966
Fragments arranged by size:
1686 1102 966 389 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 2521 2553 2720 2752 2848 2880
Size: 1258 32 1231 32 167 32 96 32
Cuts at: 2880 3292 3324 3831 3863 4180 4212 4296
Size: 412 32 507 32 317 32 84
Fragments arranged by size:
1258 1231 507 412 317 167 96 84
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 482 761 3975 4107 4296
Size: 482 279 3214 132 189
Fragments arranged by size:
3214 482 279 189 132
HindIII A'AGCT_T
Cuts at: 0 2893 3407 4296
Size: 2893 514 889
Fragments arranged by size:
2893 889 514
MfeI C'AATT_G
Cuts at: 0 594 3857 4052 4296
Size: 594 3263 195 244
Fragments arranged by size:
3263 594 244 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1859 2320 3626 4296
Size: 1859 461 1306 670
Fragments arranged by size:
1859 1306 670 461
MscI TGG'CCA
Cuts at: 0 255 1587 1634 3562 3778 4296
Size: 255 1332 47 1928 216 518
Fragments arranged by size:
1928 1332 518 255 216 47
MslI CAynn'nnrTG
Cuts at: 0 1996 2516 4296
Size: 1996 520 1780
Fragments arranged by size:
1996 1780 520
MspA1I CmG'CkG
Cuts at: 0 36 587 3063 4082 4296
Size: 36 551 2476 1019 214
Fragments arranged by size:
2476 1019 551 214 36
NcoI C'CATG_G
Cuts at: 0 2843 4296
Size: 2843 1453
NspI r_CATG'y
Cuts at: 0 101 3502 3867 4296
Size: 101 3401 365 429
Fragments arranged by size:
3401 429 365 101
PasI CC'CwG_GG
Cuts at: 0 363 4296
Size: 363 3933
PflMI CCAn_nnn'nTGG
Cuts at: 0 1212 4296
Size: 1212 3084
PfoI T'CCnGG_A
Cuts at: 0 2129 4296
Size: 2129 2167
PmlI CAC'GTG
Cuts at: 0 4099 4296
Size: 4099 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1187 1219 4296
Size: 1187 32 3077
Fragments arranged by size:
3077 1187 32
PpuMI rG'GwC_Cy
Cuts at: 0 1041 4296
Size: 1041 3255
PshAI GACnn'nnGTC
Cuts at: 0 2876 4296
Size: 2876 1420
PsiI TTA'TAA
Cuts at: 0 1256 3272 3335 4296
Size: 1256 2016 63 961
Fragments arranged by size:
2016 1256 961 63
PstI C_TGCA'G
Cuts at: 0 2252 2613 4203 4296
Size: 2252 361 1590 93
Fragments arranged by size:
2252 1590 361 93
PvuII CAG'CTG
Cuts at: 0 3063 4296
Size: 3063 1233
SanDI GG'GwC_CC
Cuts at: 0 1041 4296
Size: 1041 3255
SapI GCTCTTCn'nnn_
Cuts at: 0 1595 3814 4296
Size: 1595 2219 482
Fragments arranged by size:
2219 1595 482
SfcI C'TryA_G
Cuts at: 0 80 588 1819 2248 2609 4199 4296
Size: 80 508 1231 429 361 1590 97
Fragments arranged by size:
1590 1231 508 429 361 97 80
SmlI C'TyrA_G
Cuts at: 0 1345 1426 1618 1966 3713 4296
Size: 1345 81 192 348 1747 583
Fragments arranged by size:
1747 1345 583 348 192 81
SnaBI TAC'GTA
Cuts at: 0 1920 2650 4296
Size: 1920 730 1646
Fragments arranged by size:
1920 1646 730
SphI G_CATG'C
Cuts at: 0 3867 4296
Size: 3867 429
StuI AGG'CCT
Cuts at: 0 3920 4296
Size: 3920 376
StyI C'CwwG_G
Cuts at: 0 1854 2777 2843 4296
Size: 1854 923 66 1453
Fragments arranged by size:
1854 1453 923 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1608 4296
Size: 1608 2688
TatI w'GTAC_w
Cuts at: 0 511 981 1083 1625 2179 2763 3295 3703
Size: 511 470 102 542 554 584 532 408
Cuts at: 3703 4296
Size: 593
Fragments arranged by size:
593 584 554 542 532 511 470 408
102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 143 175 328 360 1082 1114 1567 1599
Size: 143 32 153 32 722 32 453 32
Cuts at: 1599 3591 3623 4296
Size: 1992 32 673
Fragments arranged by size:
1992 722 673 453 153 143 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 2317 4296
Size: 2317 1979
XmnI GAAnn'nnTTC
Cuts at: 0 1877 4296
Size: 1877 2419
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AloI AlwNI
ApoI AseI BamHI BanI BanII BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PstI PvuII SanDI SapI SfcI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BfrBI BmtI Bpu10I BsaHI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BssHII BssSI BstEII BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SrfI SspI SwaI Tth111I XcmI
XhoI XmaI ZraI