Restriction map for
pF1KE1497
(Linear) (Six-base) MAPSORT of: KIEE1497.seq Check: 2701 from: 1 to: 2853
KIEE1497.orf
With 182 enzymes: SgfI *
June 22, 2010 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1795 2097 2853
Size: 1795 302 756
Fragments arranged by size:
1795 756 302
AccI GT'mk_AC
Cuts at: 0 344 2831 2853
Size: 344 2487 22
Fragments arranged by size:
2487 344 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2472 2575 2734 2809 2825 2853
Size: 862 1610 103 159 75 16 28
Fragments arranged by size:
1610 862 159 103 75 28 16
AfeI AGC'GCT
Cuts at: 0 1168 2853
Size: 1168 1685
AhdI GACnn_n'nnGTC
Cuts at: 0 675 2853
Size: 675 2178
AleI CACnn'nnGTG
Cuts at: 0 1435 2790 2853
Size: 1435 1355 63
Fragments arranged by size:
1435 1355 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 1453 2853
Size: 444 1009 1400
Fragments arranged by size:
1400 1009 444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2738 2770 2853
Size: 2738 32 83
Fragments arranged by size:
2738 83 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 2853
Size: 1062 1791
ApaI G_GGCC'C
Cuts at: 0 2419 2853
Size: 2419 434
ApoI r'AATT_y
Cuts at: 0 1760 2853
Size: 1760 1093
AseI AT'TA_AT
Cuts at: 0 611 2853
Size: 611 2242
AvaI C'yCGr_G
Cuts at: 0 1331 2853
Size: 1331 1522
AvrII C'CTAG_G
Cuts at: 0 1965 2853
Size: 1965 888
BamHI G'GATC_C
Cuts at: 0 399 2853
Size: 399 2454
BanI G'GyrC_C
Cuts at: 0 1580 2148 2853
Size: 1580 568 705
Fragments arranged by size:
1580 705 568
BanII G_rGCy'C
Cuts at: 0 2419 2802 2853
Size: 2419 383 51
Fragments arranged by size:
2419 383 51
BbeI G_GCGC'C
Cuts at: 0 1584 2853
Size: 1584 1269
BbsI GAAGACnn'nnnn_
Cuts at: 0 960 2472 2541 2853
Size: 960 1512 69 312
Fragments arranged by size:
1512 960 312 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 2764 2853
Size: 2764 89
BglI GCCn_nnn'nGGC
Cuts at: 0 256 544 1820 2853
Size: 256 288 1276 1033
Fragments arranged by size:
1276 1033 288 256
Bme1580I G_kGCm'C
Cuts at: 0 2246 2419 2853
Size: 2246 173 434
Fragments arranged by size:
2246 434 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 1909 2413 2853
Size: 1909 504 440
Fragments arranged by size:
1909 504 440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 2434 2853
Size: 1429 1005 419
Fragments arranged by size:
1429 1005 419
Bpu10I CC'TnA_GC
Cuts at: 0 1055 2853
Size: 1055 1798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1462 1539 1853 2853
Size: 1025 437 77 314 1000
Fragments arranged by size:
1025 1000 437 314 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1553 2853
Size: 1553 1300
BsaAI yAC'GTr
Cuts at: 0 2752 2853
Size: 2752 101
BsaBI GATnn'nnATC
Cuts at: 0 293 398 515 661 2853
Size: 293 105 117 146 2192
Fragments arranged by size:
2192 293 146 117 105
BsaHI Gr'CG_yC
Cuts at: 0 1581 2853
Size: 1581 1272
BsaWI w'CCGG_w
Cuts at: 0 2218 2853
Size: 2218 635
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2058 2088 2853
Size: 2058 30 765
Fragments arranged by size:
2058 765 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1663 1985 2336 2853
Size: 1663 322 351 517
Fragments arranged by size:
1663 517 351 322
BseYI C'CCAG_C
Cuts at: 0 2338 2853
Size: 2338 515
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1459 2853
Size: 913 546 1394
Fragments arranged by size:
1394 913 546
BsiHKAI G_wGCw'C
Cuts at: 0 1798 2802 2853
Size: 1798 1004 51
Fragments arranged by size:
1798 1004 51
BsmI GAATG_Cn'
Cuts at: 0 643 2853
Size: 643 2210
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1645 2853
Size: 1645 1208
Bsp1286I G_dGCh'C
Cuts at: 0 1798 2246 2419 2802 2853
Size: 1798 448 173 383 51
Fragments arranged by size:
1798 448 383 173 51
BspEI T'CCGG_A
Cuts at: 0 2218 2853
Size: 2218 635
BspHI T'CATG_A
Cuts at: 0 1022 2423 2853
Size: 1022 1401 430
Fragments arranged by size:
1401 1022 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1503 1795 2097 2196 2853
Size: 1503 292 302 99 657
Fragments arranged by size:
1503 657 302 292 99
BsrBI CCG'CTC
Cuts at: 0 375 726 2853
Size: 375 351 2127
Fragments arranged by size:
2127 375 351
BsrDI GCAATG_nn'
Cuts at: 0 1049 1180 2853
Size: 1049 131 1673
Fragments arranged by size:
1673 1049 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2071 2697 2853
Size: 2071 626 156
Fragments arranged by size:
2071 626 156
BstBI TT'CG_AA
Cuts at: 0 1110 2853
Size: 1110 1743
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1149 1947 2853
Size: 1149 798 906
Fragments arranged by size:
1149 906 798
BstYI r'GATC_y
Cuts at: 0 288 399 498 1887 1959 2853
Size: 288 111 99 1389 72 894
Fragments arranged by size:
1389 894 288 111 99 72
Bsu36I CC'TnA_GG
Cuts at: 0 812 907 1853 2171 2853
Size: 812 95 946 318 682
Fragments arranged by size:
946 812 682 318 95
BtgI C'CryG_G
Cuts at: 0 125 326 2853
Size: 125 201 2527
Fragments arranged by size:
2527 201 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1269 2853
Size: 1269 1584
BtsI GCAGTG_nn'
Cuts at: 0 767 1829 1961 2001 2036 2109 2853
Size: 767 1062 132 40 35 73 744
Fragments arranged by size:
1062 767 744 132 73 40 35
ClaI AT'CG_AT
Cuts at: 0 2474 2853
Size: 2474 379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 2853
Size: 509 35 2309
Fragments arranged by size:
2309 509 35
DraI TTT'AAA
Cuts at: 0 1895 2618 2853
Size: 1895 723 235
Fragments arranged by size:
1895 723 235
DraIII CAC_nnn'GTG
Cuts at: 0 1295 2155 2853
Size: 1295 860 698
Fragments arranged by size:
1295 860 698
DrdI GACnn_nn'nnGTC
Cuts at: 0 1982 2853
Size: 1982 871
EaeI y'GGCC_r
Cuts at: 0 248 2351 2741 2853
Size: 248 2103 390 112
Fragments arranged by size:
2103 390 248 112
EcoICRI GAG'CTC
Cuts at: 0 2800 2853
Size: 2800 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1429 2434 2472 2575 2734 2809 2825
Size: 862 567 1005 38 103 159 75 16
Cuts at: 2825 2853
Size: 28
Fragments arranged by size:
1005 862 567 159 103 75 38 28
16
EcoRI G'AATT_C
Cuts at: 0 1760 2853
Size: 1760 1093
EcoRV GAT'ATC
Cuts at: 0 450 2853
Size: 450 2403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1894 2853
Size: 1894 959
HaeII r_GCGC'y
Cuts at: 0 1170 1584 2544 2853
Size: 1170 414 960 309
Fragments arranged by size:
1170 960 414 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 661 693 2421 2453 2853
Size: 168 32 461 32 1728 32 400
Fragments arranged by size:
1728 461 400 168 32 32 32
HincII GTy'rAC
Cuts at: 0 1101 2853
Size: 1101 1752
HindIII A'AGCT_T
Cuts at: 0 199 1401 2853
Size: 199 1202 1452
Fragments arranged by size:
1452 1202 199
KasI G'GCGC_C
Cuts at: 0 1580 2853
Size: 1580 1273
MfeI C'AATT_G
Cuts at: 0 1229 2853
Size: 1229 1624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 2853
Size: 995 1858
MscI TGG'CCA
Cuts at: 0 250 2353 2853
Size: 250 2103 500
Fragments arranged by size:
2103 500 250
MslI CAynn'nnrTG
Cuts at: 0 1147 1435 1945 2251 2428 2618 2790 2853
Size: 1147 288 510 306 177 190 172 63
Fragments arranged by size:
1147 510 306 288 190 177 172 63
MspA1I CmG'CkG
Cuts at: 0 775 2492 2853
Size: 775 1717 361
Fragments arranged by size:
1717 775 361
NarI GG'CG_CC
Cuts at: 0 1581 2853
Size: 1581 1272
NcoI C'CATG_G
Cuts at: 0 326 2853
Size: 326 2527
NspI r_CATG'y
Cuts at: 0 2700 2853
Size: 2700 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 2853
Size: 465 2388
PfoI T'CCnGG_A
Cuts at: 0 665 1406 2853
Size: 665 741 1447
Fragments arranged by size:
1447 741 665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 2214 2246 2853
Size: 1294 32 888 32 607
Fragments arranged by size:
1294 888 607 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1907 2853
Size: 1907 946
PspOMI G'GGCC_C
Cuts at: 0 2415 2853
Size: 2415 438
PspXI vC'TCGA_Gb
Cuts at: 0 1331 2853
Size: 1331 1522
PstI C_TGCA'G
Cuts at: 0 1164 2193 2285 2853
Size: 1164 1029 92 568
Fragments arranged by size:
1164 1029 568 92
PvuII CAG'CTG
Cuts at: 0 2492 2853
Size: 2492 361
SacI G_AGCT'C
Cuts at: 0 2802 2853
Size: 2802 51
SexAI A'CCwGG_T
Cuts at: 0 2151 2853
Size: 2151 702
SfcI C'TryA_G
Cuts at: 0 1085 1160 2189 2281 2853
Size: 1085 75 1029 92 572
Fragments arranged by size:
1085 1029 572 92 75
SfoI GGC'GCC
Cuts at: 0 1582 2853
Size: 1582 1271
SmlI C'TyrA_G
Cuts at: 0 1040 1331 1477 1518 1868 2853
Size: 1040 291 146 41 350 985
Fragments arranged by size:
1040 985 350 291 146 41
SnaBI TAC'GTA
Cuts at: 0 2752 2853
Size: 2752 101
SspI AAT'ATT
Cuts at: 0 44 2853
Size: 44 2809
StuI AGG'CCT
Cuts at: 0 939 2853
Size: 939 1914
StyI C'CwwG_G
Cuts at: 0 311 326 1965 2001 2238 2411 2853
Size: 311 15 1639 36 237 173 442
Fragments arranged by size:
1639 442 311 237 173 36 15
SwaI ATTT'AAAT
Cuts at: 0 2618 2853
Size: 2618 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 587 1447 1897 2046 2853
Size: 587 860 450 149 807
Fragments arranged by size:
860 807 587 450 149
TatI w'GTAC_w
Cuts at: 0 980 2853
Size: 980 1873
XbaI T'CTAG_A
Cuts at: 0 798 2853
Size: 798 2055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 1740 2346 2679 2853
Size: 759 981 606 333 174
Fragments arranged by size:
981 759 606 333 174
XhoI C'TCGA_G
Cuts at: 0 1331 2853
Size: 1331 1522
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI AloI
AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EcoICRI Eco57MI EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NspI PflMI PfoI PpiI PshAI
PspOMI PspXI PstI PvuII SacI SexAI SfcI SfoI
SmlI SnaBI SspI StuI StyI SwaI TaqII TatI
XbaI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI ApaLI AscI
AsiSI BaeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsiEI BsiWI BsrFI BsrGI BssHII
BssSI BstEII BstZ17I EagI EarI EciI EcoNI EcoO109I
FseI FspI FspAI HpaI KpnI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpuMI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SpeI SphI SrfI TstI Tth111I XmaI XmnI
ZraI