Restriction map for
pF1KE1444
(Linear) (Six-base) MAPSORT of: KIEE1444.seq Check: 5571 from: 1 to: 822
KIEE1444.orf
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AatII G_ACGT'C
Cuts at: 0 49 822
Size: 49 773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 822
Size: 199 623
ApoI r'AATT_y
Cuts at: 0 602 751 822
Size: 602 149 71
Fragments arranged by size:
602 149 71
BamHI G'GATC_C
Cuts at: 0 479 822
Size: 479 343
BanII G_rGCy'C
Cuts at: 0 247 822
Size: 247 575
BciVI GTATCCnnnnn_n'
Cuts at: 0 212 822
Size: 212 610
Bme1580I G_kGCm'C
Cuts at: 0 584 822
Size: 584 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 655 822
Size: 572 83 167
Fragments arranged by size:
572 167 83
BsaHI Gr'CG_yC
Cuts at: 0 46 596 822
Size: 46 550 226
Fragments arranged by size:
550 226 46
BsaWI w'CCGG_w
Cuts at: 0 413 822
Size: 413 409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 822
Size: 751 71
BsiHKAI G_wGCw'C
Cuts at: 0 18 822
Size: 18 804
BsmI GAATG_Cn'
Cuts at: 0 524 822
Size: 524 298
Bsp1286I G_dGCh'C
Cuts at: 0 18 247 584 822
Size: 18 229 337 238
Fragments arranged by size:
337 238 229 18
BspEI T'CCGG_A
Cuts at: 0 413 822
Size: 413 409
BstYI r'GATC_y
Cuts at: 0 479 822
Size: 479 343
BtgI C'CryG_G
Cuts at: 0 483 822
Size: 483 339
BtsI GCAGTG_nn'
Cuts at: 0 319 434 459 822
Size: 319 115 25 363
Fragments arranged by size:
363 319 115 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 496 822
Size: 496 326
EaeI y'GGCC_r
Cuts at: 0 508 822
Size: 508 314
EarI CTCTTCn'nnn_
Cuts at: 0 6 822
Size: 6 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 572 655 822
Size: 199 373 83 167
Fragments arranged by size:
373 199 167 83
EcoO109I rG'GnC_Cy
Cuts at: 0 183 406 822
Size: 183 223 416
Fragments arranged by size:
416 223 183
EcoRI G'AATT_C
Cuts at: 0 602 822
Size: 602 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 31 37 63 822
Size: 5 26 6 26 759
Fragments arranged by size:
759 26 26 6 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 822
Size: 98 724
MscI TGG'CCA
Cuts at: 0 510 822
Size: 510 312
MslI CAynn'nnrTG
Cuts at: 0 536 822
Size: 536 286
MspA1I CmG'CkG
Cuts at: 0 82 822
Size: 82 740
PflMI CCAn_nnn'nTGG
Cuts at: 0 777 822
Size: 777 45
PfoI T'CCnGG_A
Cuts at: 0 611 822
Size: 611 211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 822
Size: 343 32 447
Fragments arranged by size:
447 343 32
PpuMI rG'GwC_Cy
Cuts at: 0 183 406 822
Size: 183 223 416
Fragments arranged by size:
416 223 183
PsiI TTA'TAA
Cuts at: 0 227 822
Size: 227 595
PstI C_TGCA'G
Cuts at: 0 81 314 822
Size: 81 233 508
Fragments arranged by size:
508 233 81
SapI GCTCTTCn'nnn_
Cuts at: 0 6 822
Size: 6 816
SfcI C'TryA_G
Cuts at: 0 77 310 822
Size: 77 233 512
Fragments arranged by size:
512 233 77
SspI AAT'ATT
Cuts at: 0 642 822
Size: 642 180
TatI w'GTAC_w
Cuts at: 0 464 822
Size: 464 358
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 822
Size: 92 32 698
Fragments arranged by size:
698 92 32
ZraI GAC'GTC
Cuts at: 0 47 822
Size: 47 775
Enzymes that do cut:
AatII AcuI ApoI BamHI BanII BciVI Bme1580I BpmI
BsaHI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspEI BstYI
BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I EcoRI
Hin4I MmeI MscI MslI MspA1I PflMI PfoI PpiI
PpuMI PsiI PstI SapI SfcI SspI TatI TstI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI