Restriction map for
pF1KE1327
(Linear) (Six-base) MAPSORT of: KIEE1327.seq Check: 729 from: 1 to: 1407
KIEE1327.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1222 1261 1407
Size: 1222 39 146
Fragments arranged by size:
1222 146 39
Acc65I G'GTAC_C
Cuts at: 0 956 1407
Size: 956 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 938 1223 1407
Size: 490 448 285 184
Fragments arranged by size:
490 448 285 184
AflIII A'CryG_T
Cuts at: 0 714 769 1407
Size: 714 55 638
Fragments arranged by size:
714 638 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 650 682 1407
Size: 650 32 725
Fragments arranged by size:
725 650 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 1246 1300 1407
Size: 384 862 54 107
Fragments arranged by size:
862 384 107 54
ApaI G_GGCC'C
Cuts at: 0 21 643 1407
Size: 21 622 764
Fragments arranged by size:
764 622 21
ApoI r'AATT_y
Cuts at: 0 703 1407
Size: 703 704
AvaI C'yCGr_G
Cuts at: 0 82 1309 1407
Size: 82 1227 98
Fragments arranged by size:
1227 98 82
BamHI G'GATC_C
Cuts at: 0 1043 1407
Size: 1043 364
BanI G'GyrC_C
Cuts at: 0 956 1407
Size: 956 451
BanII G_rGCy'C
Cuts at: 0 21 366 539 643 826 1407
Size: 21 345 173 104 183 581
Fragments arranged by size:
581 345 183 173 104 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 798 1407
Size: 798 609
BbvCI CC'TCA_GC
Cuts at: 0 1318 1407
Size: 1318 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1128 1162 1407
Size: 1128 34 245
Fragments arranged by size:
1128 245 34
BglI GCCn_nnn'nGGC
Cuts at: 0 344 1012 1407
Size: 344 668 395
Fragments arranged by size:
668 395 344
BglII A'GATC_T
Cuts at: 0 509 1407
Size: 509 898
Bme1580I G_kGCm'C
Cuts at: 0 21 643 1082 1173 1194 1407
Size: 21 622 439 91 21 213
Fragments arranged by size:
622 439 213 91 21 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 424 1407
Size: 36 388 983
Fragments arranged by size:
983 388 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 498 1407
Size: 498 909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 622 1369 1407
Size: 350 272 747 38
Fragments arranged by size:
747 350 272 38
Bpu10I CC'TnA_GC
Cuts at: 0 1318 1407
Size: 1318 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 897 1407
Size: 897 510
BsaAI yAC'GTr
Cuts at: 0 772 1287 1407
Size: 772 515 120
Fragments arranged by size:
772 515 120
BsaWI w'CCGG_w
Cuts at: 0 529 1407
Size: 529 878
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1199 1229 1407
Size: 1199 30 178
Fragments arranged by size:
1199 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 884 1223 1407
Size: 884 339 184
Fragments arranged by size:
884 339 184
BseYI C'CCAG_C
Cuts at: 0 1250 1356 1365 1407
Size: 1250 106 9 42
Fragments arranged by size:
1250 106 42 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 541 1259 1407
Size: 85 456 718 148
Fragments arranged by size:
718 456 148 85
BsiHKAI G_wGCw'C
Cuts at: 0 826 880 1407
Size: 826 54 527
Fragments arranged by size:
826 527 54
Bsp1286I G_dGCh'C
Cuts at: 0 21 366 539 643 826 880 1082 1173
Size: 21 345 173 104 183 54 202 91
Cuts at: 1173 1194 1407
Size: 21 213
Fragments arranged by size:
345 213 202 183 173 104 91 54
21 21
BspHI T'CATG_A
Cuts at: 0 90 1379 1407
Size: 90 1289 28
Fragments arranged by size:
1289 90 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 950 1222 1231 1261 1407
Size: 107 843 272 9 30 146
Fragments arranged by size:
843 272 146 107 30 9
BsrDI GCAATG_nn'
Cuts at: 0 1333 1407
Size: 1333 74
BssSI C'ACGA_G
Cuts at: 0 202 1084 1407
Size: 202 882 323
Fragments arranged by size:
882 323 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1140 1237 1407
Size: 1140 97 170
Fragments arranged by size:
1140 170 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 184 190 280 1407
Size: 12 172 6 90 1127
Fragments arranged by size:
1127 172 90 12 6
BstYI r'GATC_y
Cuts at: 0 509 1043 1407
Size: 509 534 364
Fragments arranged by size:
534 509 364
BtgI C'CryG_G
Cuts at: 0 35 126 165 183 189 240 267 279
Size: 35 91 39 18 6 51 27 12
Cuts at: 279 339 470 1407
Size: 60 131 937
Fragments arranged by size:
937 131 91 60 51 39 35 27
18 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 478 1153 1407
Size: 478 675 254
Fragments arranged by size:
675 478 254
BtsI GCAGTG_nn'
Cuts at: 0 1220 1227 1407
Size: 1220 7 180
Fragments arranged by size:
1220 180 7
DraIII CAC_nnn'GTG
Cuts at: 0 30 1407
Size: 30 1377
DrdI GACnn_nn'nnGTC
Cuts at: 0 86 1407
Size: 86 1321
EaeI y'GGCC_r
Cuts at: 0 2 32 129 168 186 192 270 633
Size: 2 30 97 39 18 6 78 363
Cuts at: 633 1004 1407
Size: 371 403
Fragments arranged by size:
403 371 363 97 78 39 30 18
6 2
EarI CTCTTCn'nnn_
Cuts at: 0 463 856 1407
Size: 463 393 551
Fragments arranged by size:
551 463 393
EcoICRI GAG'CTC
Cuts at: 0 824 1407
Size: 824 583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 490 622 938 1223 1369 1407
Size: 350 140 132 316 285 146 38
Fragments arranged by size:
350 316 285 146 140 132 38
EcoO109I rG'GnC_Cy
Cuts at: 0 17 18 381 639 640 1243 1254 1407
Size: 17 1 363 258 1 603 11 153
Fragments arranged by size:
603 363 258 153 17 11 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1028 1060 1407
Size: 1028 32 347
Fragments arranged by size:
1028 347 32
KpnI G_GTAC'C
Cuts at: 0 960 1407
Size: 960 447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1407
Size: 1071 336
MscI TGG'CCA
Cuts at: 0 4 131 170 188 194 272 635 1006
Size: 4 127 39 18 6 78 363 371
Cuts at: 1006 1407
Size: 401
Fragments arranged by size:
401 371 363 127 78 39 18 6
4
MslI CAynn'nnrTG
Cuts at: 0 149 176 182 188 239 251 278 320
Size: 149 27 6 6 51 12 27 42
Cuts at: 320 326 338 740 746 770 785 1384 1407
Size: 6 12 402 6 24 15 599 23
Fragments arranged by size:
599 402 149 51 42 27 27 24
23 15 12 12 6 6 6 6
NcoI C'CATG_G
Cuts at: 0 126 165 183 189 240 267 279 339
Size: 126 39 18 6 51 27 12 60
Cuts at: 339 1407
Size: 1068
Fragments arranged by size:
1068 126 60 51 39 27 18 12
6
NspI r_CATG'y
Cuts at: 0 718 1407
Size: 718 689
PciI A'CATG_T
Cuts at: 0 714 1407
Size: 714 693
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 600 1407
Size: 30 570 807
Fragments arranged by size:
807 570 30
PmlI CAC'GTG
Cuts at: 0 772 1407
Size: 772 635
PpuMI rG'GwC_Cy
Cuts at: 0 381 1243 1254 1407
Size: 381 862 11 153
Fragments arranged by size:
862 381 153 11
PspOMI G'GGCC_C
Cuts at: 0 17 639 1407
Size: 17 622 768
Fragments arranged by size:
768 622 17
PstI C_TGCA'G
Cuts at: 0 1126 1272 1407
Size: 1126 146 135
Fragments arranged by size:
1126 146 135
SacI G_AGCT'C
Cuts at: 0 826 1407
Size: 826 581
SfcI C'TryA_G
Cuts at: 0 1122 1268 1407
Size: 1122 146 139
Fragments arranged by size:
1122 146 139
SmlI C'TyrA_G
Cuts at: 0 1402 1407
Size: 1402 5
SnaBI TAC'GTA
Cuts at: 0 1287 1407
Size: 1287 120
StuI AGG'CCT
Cuts at: 0 11 1407
Size: 11 1396
StyI C'CwwG_G
Cuts at: 0 59 126 165 183 189 240 267 279
Size: 59 67 39 18 6 51 27 12
Cuts at: 279 339 809 1407
Size: 60 470 598
Fragments arranged by size:
598 470 67 60 59 51 39 27
18 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 1407
Size: 18 1389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1199 1231 1407
Size: 1199 32 176
Fragments arranged by size:
1199 176 32
Tth111I GACn'n_nGTC
Cuts at: 0 731 1407
Size: 731 676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 313 346 651 1407
Size: 313 33 305 756
Fragments arranged by size:
756 313 305 33
Enzymes that do cut:
AarI Acc65I AcuI AflIII AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I KpnI MmeI MscI MslI NcoI NspI PciI
PflMI PmlI PpuMI PspOMI PstI SacI SfcI SmlI
SnaBI StuI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BclI BfrBI BlpI BmgBI BmtI BpuEI BsaBI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI XmaI XmnI ZraI