(Linear) (Six-base) MAPSORT of: KIEE1237.seq  Check: 9062  from: 1  to: 1260



KIEE1237.orf

 With 182 enzymes: SgfI * 

                        November 2, 2015 11:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195    1087    1260 
   Size:        195     892     173

  Fragments arranged by size: 

                 892     195     173
 
AflII C'TTAA_G

Cuts at:      0    1081    1260 
   Size:       1081     179
 
AflIII A'CryG_T

Cuts at:      0      46     897    1260 
   Size:         46     851     363

  Fragments arranged by size: 

                 851     363      46
 
AlwNI CAG_nnn'CTG

Cuts at:      0     148    1158    1260 
   Size:        148    1010     102

  Fragments arranged by size: 

                1010     148     102
 
ApoI r'AATT_y

Cuts at:      0     526     616     976    1211    1260 
   Size:        526      90     360     235      49

  Fragments arranged by size: 

                 526     360     235      90      49
 
BamHI G'GATC_C

Cuts at:      0     717    1260 
   Size:        717     543
 
BclI T'GATC_A

Cuts at:      0     417    1137    1260 
   Size:        417     720     123

  Fragments arranged by size: 

                 720     417     123
 
BlpI GC'TnA_GC

Cuts at:      0     262    1260 
   Size:        262     998
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     238    1260 
   Size:        238    1022
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     964    1165    1253    1260 
   Size:        208     756     201      88       7

  Fragments arranged by size: 

                 756     208     201      88       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157    1260 
   Size:        157    1103
 
BsaBI GATnn'nnATC

Cuts at:      0     167    1260 
   Size:        167    1093
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     961    1260 
   Size:        961     299
 
BseYI C'CCAG_C

Cuts at:      0     995    1260 
   Size:        995     265
 
BspHI T'CATG_A

Cuts at:      0     519    1260 
   Size:        519     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     995    1260 
   Size:        995     265
 
BstYI r'GATC_y

Cuts at:      0     717    1260 
   Size:        717     543
 
DraI TTT'AAA

Cuts at:      0     531     575    1260 
   Size:        531      44     685

  Fragments arranged by size: 

                 685     531      44
 
DraIII CAC_nnn'GTG

Cuts at:      0     117     294    1260 
   Size:        117     177     966

  Fragments arranged by size: 

                 966     177     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     407     602     695     842    1112    1260 
   Size:        407     195      93     147     270     148

  Fragments arranged by size: 

                 407     270     195     148     147      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     208     964    1087    1165    1253    1260 
   Size:        195      13     756     123      78      88       7

  Fragments arranged by size: 

                 756     195     123      88      78      13       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1220    1260 
   Size:       1220      40
 
EcoRI G'AATT_C

Cuts at:      0     976    1211    1260 
   Size:        976     235      49

  Fragments arranged by size: 

                 976     235      49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     898    1260 
   Size:        898     362
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     993    1025    1260 
   Size:        993      32     235

  Fragments arranged by size: 

                 993     235      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     800    1260 
   Size:        344     456     460

  Fragments arranged by size: 

                 460     456     344
 
MspA1I CmG'CkG

Cuts at:      0    1231    1260 
   Size:       1231      29
 
NspI r_CATG'y

Cuts at:      0      50     901    1260 
   Size:         50     851     359

  Fragments arranged by size: 

                 851     359      50
 
PacI TTA_AT'TAA

Cuts at:      0     353    1260 
   Size:        353     907
 
PciI A'CATG_T

Cuts at:      0      46     897    1260 
   Size:         46     851     363

  Fragments arranged by size: 

                 851     363      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     148    1260 
   Size:        148    1112
 
PfoI T'CCnGG_A

Cuts at:      0     779    1260 
   Size:        779     481
 
PvuII CAG'CTG

Cuts at:      0    1231    1260 
   Size:       1231      29
 
SapI GCTCTTCn'nnn_

Cuts at:      0     407    1112    1260 
   Size:        407     705     148

  Fragments arranged by size: 

                 705     407     148
 
SfcI C'TryA_G

Cuts at:      0      22    1260 
   Size:         22    1238
 
SmlI C'TyrA_G

Cuts at:      0     172    1081    1260 
   Size:        172     909     179

  Fragments arranged by size: 

                 909     179     172
 
SspI AAT'ATT

Cuts at:      0     108     571    1056    1260 
   Size:        108     463     485     204

  Fragments arranged by size: 

                 485     463     204     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     680    1260 
   Size:        680     580
 
TatI w'GTAC_w

Cuts at:      0      99    1260 
   Size:         99    1161
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    AlwNI     ApoI    BamHI     BclI     BlpI
     BplI     BpmI    BpuEI    BsaBI    BseRI    BseYI    BspHI    BstXI
    BstYI     DraI   DraIII     EarI  Eco57MI    EcoNI    EcoRI     FalI
    Hin4I     MmeI   MspA1I     NspI     PacI     PciI    PflMI     PfoI
    PvuII     SapI     SfcI     SmlI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PasI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI