(Linear) (Six-base) MAPSORT of: KIEE1098.seq  Check: 7013  from: 1  to: 945



KIEE1098.orf

 With 182 enzymes: SgfI * 

                        November 19, 2009 09:54  ..

ApoI r'AATT_y

Cuts at:      0      34     301     569     945 
   Size:         34     267     268     376

  Fragments arranged by size: 

                 376     268     267      34
 
AseI AT'TA_AT

Cuts at:      0     530     539     680     945 
   Size:        530       9     141     265

  Fragments arranged by size: 

                 530     265     141       9
 
BanI G'GyrC_C

Cuts at:      0     442     945 
   Size:        442     503
 
BanII G_rGCy'C

Cuts at:      0     824     945 
   Size:        824     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     302     945 
   Size:        302     643
 
BbvCI CC'TCA_GC

Cuts at:      0     347     945 
   Size:        347     598
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     175     945 
   Size:        175     770
 
BfrBI ATG'CAT

Cuts at:      0     454     945 
   Size:        454     491
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     745     945 
   Size:        257     488     200

  Fragments arranged by size: 

                 488     257     200
 
Bpu10I CC'TnA_GC

Cuts at:      0     347     945 
   Size:        347     598
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     423     945 
   Size:        423     522
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     683     686     945 
   Size:        683       3     259

  Fragments arranged by size: 

                 683     259       3
 
BsmI GAATG_Cn'

Cuts at:      0     329     945 
   Size:        329     616
 
Bsp1286I G_dGCh'C

Cuts at:      0     824     945 
   Size:        824     121
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     631     945 
   Size:        631     314
 
BsrDI GCAATG_nn'

Cuts at:      0      77     945 
   Size:         77     868
 
BstYI r'GATC_y

Cuts at:      0      73     945 
   Size:         73     872
 
Bsu36I CC'TnA_GG

Cuts at:      0      41     945 
   Size:         41     904
 
EarI CTCTTCn'nnn_

Cuts at:      0     258     945 
   Size:        258     687
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     745     945 
   Size:        257     488     200

  Fragments arranged by size: 

                 488     257     200
 
HaeII r_GCGC'y

Cuts at:      0     549     945 
   Size:        549     396
 
MslI CAynn'nnrTG

Cuts at:      0     223     945 
   Size:        223     722
 
MspA1I CmG'CkG

Cuts at:      0     378     945 
   Size:        378     567
 
NsiI A_TGCA'T

Cuts at:      0     456     945 
   Size:        456     489
 
NspI r_CATG'y

Cuts at:      0     466     669     945 
   Size:        466     203     276

  Fragments arranged by size: 

                 466     276     203
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     506     538     945 
   Size:        506      32     407

  Fragments arranged by size: 

                 506     407      32
 
PvuII CAG'CTG

Cuts at:      0     378     945 
   Size:        378     567
 
ScaI AGT'ACT

Cuts at:      0     524     945 
   Size:        524     421
 
SfcI C'TryA_G

Cuts at:      0     656     945 
   Size:        656     289
 
SphI G_CATG'C

Cuts at:      0     466     945 
   Size:        466     479
 
SspI AAT'ATT

Cuts at:      0     537     945 
   Size:        537     408
 
StuI AGG'CCT

Cuts at:      0     740     945 
   Size:        740     205
 
StyI C'CwwG_G

Cuts at:      0     365     945 
   Size:        365     580
 
TatI w'GTAC_w

Cuts at:      0     422     522     945 
   Size:        422     100     423

  Fragments arranged by size: 

                 423     422     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     192     224     945 
   Size:        192      32     721

  Fragments arranged by size: 

                 721     192      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      84     945 
   Size:         84     861
 
XmnI GAAnn'nnTTC

Cuts at:      0     267     945 
   Size:        267     678
 

 Enzymes that do cut:

     ApoI     AseI     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI
     BpmI   Bpu10I     BsaI    BseRI     BsmI Bsp1286I    BspMI    BsrDI
    BstYI   Bsu36I     EarI  Eco57MI    HaeII     MslI   MspA1I     NsiI
     NspI     PsrI    PvuII     ScaI     SfcI     SphI     SspI     StuI
     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI