Restriction map for
pF1KE1092
(Linear) (Six-base) MAPSORT of: KIEE1092.seq Check: 9436 from: 1 to: 4644
KIEE1092.orf
With 182 enzymes: SgfI *
October 26, 2009 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1137 1708 4644
Size: 1137 571 2936
Fragments arranged by size:
2936 1137 571
AccI GT'mk_AC
Cuts at: 0 1078 1794 4644
Size: 1078 716 2850
Fragments arranged by size:
2850 1078 716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 974 1492 1853 2597 3832 4261 4637
Size: 134 840 518 361 744 1235 429 376
Cuts at: 4637 4644
Size: 7
Fragments arranged by size:
1235 840 744 518 429 376 361 134
7
AfeI AGC'GCT
Cuts at: 0 4489 4644
Size: 4489 155
AflIII A'CryG_T
Cuts at: 0 3620 4064 4564 4644
Size: 3620 444 500 80
Fragments arranged by size:
3620 500 444 80
AhdI GACnn_n'nnGTC
Cuts at: 0 153 3825 4644
Size: 153 3672 819
Fragments arranged by size:
3672 819 153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 3008 3040 4644
Size: 1106 32 1870 32 1604
Fragments arranged by size:
1870 1604 1106 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 184 2239 3411 3951 4644
Size: 184 2055 1172 540 693
Fragments arranged by size:
2055 1172 693 540 184
ApoI r'AATT_y
Cuts at: 0 189 242 386 720 1836 1863 2706 3017
Size: 189 53 144 334 1116 27 843 311
Cuts at: 3017 3938 4644
Size: 921 706
Fragments arranged by size:
1116 921 843 706 334 311 189 144
53 27
BanI G'GyrC_C
Cuts at: 0 786 2943 4644
Size: 786 2157 1701
Fragments arranged by size:
2157 1701 786
BanII G_rGCy'C
Cuts at: 0 3560 3992 4644
Size: 3560 432 652
Fragments arranged by size:
3560 652 432
BbsI GAAGACnn'nnnn_
Cuts at: 0 1660 2918 3101 3187 3824 4625 4644
Size: 1660 1258 183 86 637 801 19
Fragments arranged by size:
1660 1258 801 637 183 86 19
BbvCI CC'TCA_GC
Cuts at: 0 1617 1752 4644
Size: 1617 135 2892
Fragments arranged by size:
2892 1617 135
BciVI GTATCCnnnnn_n'
Cuts at: 0 93 4644
Size: 93 4551
BclI T'GATC_A
Cuts at: 0 426 1205 4644
Size: 426 779 3439
Fragments arranged by size:
3439 779 426
BfrBI ATG'CAT
Cuts at: 0 2405 4644
Size: 2405 2239
BlpI GC'TnA_GC
Cuts at: 0 2693 4644
Size: 2693 1951
Bme1580I G_kGCm'C
Cuts at: 0 1215 1880 2685 4644
Size: 1215 665 805 1959
Fragments arranged by size:
1959 1215 805 665
BmrI ACTGGGnnnn_n'
Cuts at: 0 724 1209 2007 3657 4399 4644
Size: 724 485 798 1650 742 245
Fragments arranged by size:
1650 798 742 724 485 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1981 2633 2987 4644
Size: 1027 954 652 354 1657
Fragments arranged by size:
1657 1027 954 652 354
Bpu10I CC'TnA_GC
Cuts at: 0 1617 1752 4484 4644
Size: 1617 135 2732 160
Fragments arranged by size:
2732 1617 160 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 854 3163 3734 3752 4644
Size: 821 33 2309 571 18 892
Fragments arranged by size:
2309 892 821 571 33 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 512 1999 3879 4644
Size: 512 1487 1880 765
Fragments arranged by size:
1880 1487 765 512
BsaAI yAC'GTr
Cuts at: 0 4567 4644
Size: 4567 77
BsaHI Gr'CG_yC
Cuts at: 0 961 4644
Size: 961 3683
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2363 2393 4644
Size: 2363 30 2251
Fragments arranged by size:
2363 2251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1136 1952 4644
Size: 1136 816 2692
Fragments arranged by size:
2692 1136 816
BseYI C'CCAG_C
Cuts at: 0 2018 2300 3311 4110 4644
Size: 2018 282 1011 799 534
Fragments arranged by size:
2018 1011 799 534 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 2343 2750 3816 4644
Size: 77 2266 407 1066 828
Fragments arranged by size:
2266 1066 828 407 77
BsiHKAI G_wGCw'C
Cuts at: 0 4196 4644
Size: 4196 448
BsmI GAATG_Cn'
Cuts at: 0 170 1353 2407 2905 4644
Size: 170 1183 1054 498 1739
Fragments arranged by size:
1739 1183 1054 498 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2008 4218 4644
Size: 2008 2210 426
Fragments arranged by size:
2210 2008 426
Bsp1286I G_dGCh'C
Cuts at: 0 1215 1880 2685 3560 3992 4196 4644
Size: 1215 665 805 875 432 204 448
Fragments arranged by size:
1215 875 805 665 448 432 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1137 1708 2528 4644
Size: 1137 571 820 2116
Fragments arranged by size:
2116 1137 820 571
BsrBI CCG'CTC
Cuts at: 0 3800 4644
Size: 3800 844
BsrDI GCAATG_nn'
Cuts at: 0 193 788 2186 4644
Size: 193 595 1398 2458
Fragments arranged by size:
2458 1398 595 193
BsrGI T'GTAC_A
Cuts at: 0 1722 4644
Size: 1722 2922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1805 4644
Size: 1805 2839
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1647 2026 3311 4644
Size: 1647 379 1285 1333
Fragments arranged by size:
1647 1333 1285 379
BstYI r'GATC_y
Cuts at: 0 181 231 1612 3684 4644
Size: 181 50 1381 2072 960
Fragments arranged by size:
2072 1381 960 181 50
BstZ17I GTA'TAC
Cuts at: 0 1795 4644
Size: 1795 2849
Bsu36I CC'TnA_GG
Cuts at: 0 3738 4644
Size: 3738 906
BtgI C'CryG_G
Cuts at: 0 2597 4644
Size: 2597 2047
BtsI GCAGTG_nn'
Cuts at: 0 1407 4644
Size: 1407 3237
DraI TTT'AAA
Cuts at: 0 444 1190 4139 4644
Size: 444 746 2949 505
Fragments arranged by size:
2949 746 505 444
DraIII CAC_nnn'GTG
Cuts at: 0 1103 1591 2041 4644
Size: 1103 488 450 2603
Fragments arranged by size:
2603 1103 488 450
EaeI y'GGCC_r
Cuts at: 0 852 2438 4644
Size: 852 1586 2206
Fragments arranged by size:
2206 1586 852
EarI CTCTTCn'nnn_
Cuts at: 0 339 2378 2984 3167 3314 3519 3668 4079
Size: 339 2039 606 183 147 205 149 411
Cuts at: 4079 4644
Size: 565
Fragments arranged by size:
2039 606 565 411 339 205 183 149
147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 4644
Size: 19 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 974 1027 1492 1853 1981 2597 2633
Size: 134 840 53 465 361 128 616 36
Cuts at: 2633 2987 3832 4261 4637 4644
Size: 354 845 429 376 7
Fragments arranged by size:
845 840 616 465 429 376 361 354
134 128 53 36 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1045 4644
Size: 1045 3599
EcoO109I rG'GnC_Cy
Cuts at: 0 2620 3408 4644
Size: 2620 788 1236
Fragments arranged by size:
2620 1236 788
EcoRI G'AATT_C
Cuts at: 0 242 720 1863 2706 3017 3938 4644
Size: 242 478 1143 843 311 921 706
Fragments arranged by size:
1143 921 843 706 478 311 242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3127 4224 4644
Size: 3127 1097 420
Fragments arranged by size:
3127 1097 420
HaeII r_GCGC'y
Cuts at: 0 4118 4491 4644
Size: 4118 373 153
Fragments arranged by size:
4118 373 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 139 171 1507 1539 3174 3206
Size: 4 32 103 32 1336 32 1635 32
Cuts at: 3206 3268 3280 3300 3312 3412 3444 3449 3481
Size: 62 12 20 12 100 32 5 32
Cuts at: 3481 3811 3843 4221 4248 4253 4280 4391 4423
Size: 330 32 378 27 5 27 111 32
Cuts at: 4423 4644
Size: 221
Fragments arranged by size:
1635 1336 378 330 221 111 103 100
62 32 32 32 32 32 32 32
32 27 27 20 12 12 5 5
4
HincII GTy'rAC
Cuts at: 0 935 1268 2062 4562 4644
Size: 935 333 794 2500 82
Fragments arranged by size:
2500 935 794 333 82
HindIII A'AGCT_T
Cuts at: 0 2831 3109 4201 4644
Size: 2831 278 1092 443
Fragments arranged by size:
2831 1092 443 278
HpaI GTT'AAC
Cuts at: 0 4562 4644
Size: 4562 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 2761 4197 4434 4644
Size: 527 2234 1436 237 210
Fragments arranged by size:
2234 1436 527 237 210
MscI TGG'CCA
Cuts at: 0 854 2440 4644
Size: 854 1586 2204
Fragments arranged by size:
2204 1586 854
MslI CAynn'nnrTG
Cuts at: 0 320 3500 4398 4644
Size: 320 3180 898 246
Fragments arranged by size:
3180 898 320 246
MspA1I CmG'CkG
Cuts at: 0 1006 1817 1990 4110 4644
Size: 1006 811 173 2120 534
Fragments arranged by size:
2120 1006 811 534 173
NcoI C'CATG_G
Cuts at: 0 2597 4644
Size: 2597 2047
NdeI CA'TA_TG
Cuts at: 0 1272 4644
Size: 1272 3372
NsiI A_TGCA'T
Cuts at: 0 2407 4644
Size: 2407 2237
NspI r_CATG'y
Cuts at: 0 156 1338 1506 2026 2409 3624 4068 4644
Size: 156 1182 168 520 383 1215 444 576
Fragments arranged by size:
1215 1182 576 520 444 383 168 156
PciI A'CATG_T
Cuts at: 0 3620 4064 4644
Size: 3620 444 580
Fragments arranged by size:
3620 580 444
PflMI CCAn_nnn'nTGG
Cuts at: 0 253 1383 4644
Size: 253 1130 3261
Fragments arranged by size:
3261 1130 253
PfoI T'CCnGG_A
Cuts at: 0 3601 4644
Size: 3601 1043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3206 3238 4644
Size: 3206 32 1406
Fragments arranged by size:
3206 1406 32
PpuMI rG'GwC_Cy
Cuts at: 0 2620 3408 4644
Size: 2620 788 1236
Fragments arranged by size:
2620 1236 788
PsiI TTA'TAA
Cuts at: 0 2279 4644
Size: 2279 2365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4031 4063 4644
Size: 4031 32 581
Fragments arranged by size:
4031 581 32
PstI C_TGCA'G
Cuts at: 0 1719 1810 1989 4644
Size: 1719 91 179 2655
Fragments arranged by size:
2655 1719 179 91
PvuII CAG'CTG
Cuts at: 0 1006 1817 1990 4110 4644
Size: 1006 811 173 2120 534
Fragments arranged by size:
2120 1006 811 534 173
SapI GCTCTTCn'nnn_
Cuts at: 0 3314 3668 4079 4644
Size: 3314 354 411 565
Fragments arranged by size:
3314 565 411 354
ScaI AGT'ACT
Cuts at: 0 2772 3747 4644
Size: 2772 975 897
Fragments arranged by size:
2772 975 897
SfcI C'TryA_G
Cuts at: 0 1715 1806 1812 1985 2253 2699 3542 3789
Size: 1715 91 6 173 268 446 843 247
Cuts at: 3789 4644
Size: 855
Fragments arranged by size:
1715 855 843 446 268 247 173 91
6
SmlI C'TyrA_G
Cuts at: 0 836 869 3142 3749 3767 4644
Size: 836 33 2273 607 18 877
Fragments arranged by size:
2273 877 836 607 33 18
SpeI A'CTAG_T
Cuts at: 0 1234 4644
Size: 1234 3410
SspI AAT'ATT
Cuts at: 0 738 2191 2592 4644
Size: 738 1453 401 2052
Fragments arranged by size:
2052 1453 738 401
StuI AGG'CCT
Cuts at: 0 3712 4644
Size: 3712 932
StyI C'CwwG_G
Cuts at: 0 2597 2812 3255 3668 3933 4644
Size: 2597 215 443 413 265 711
Fragments arranged by size:
2597 711 443 413 265 215
TatI w'GTAC_w
Cuts at: 0 1722 2770 3262 3745 4244 4644
Size: 1722 1048 492 483 499 400
Fragments arranged by size:
1722 1048 499 492 483 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2363 2395 4115 4147 4644
Size: 2363 32 1720 32 497
Fragments arranged by size:
2363 1720 497 32 32
XbaI T'CTAG_A
Cuts at: 0 3228 4644
Size: 3228 1416
XmnI GAAnn'nnTTC
Cuts at: 0 1840 1863 2509 2640 3110 3224 3382 3821
Size: 1840 23 646 131 470 114 158 439
Cuts at: 3821 4644
Size: 823
Fragments arranged by size:
1840 823 646 470 439 158 131 114
23
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AloI AlwNI
ApoI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PciI PflMI PfoI PpiI PpuMI
PsiI PsrI PstI PvuII SapI ScaI SfcI SmlI
SpeI SspI StuI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BglI BglII BmgBI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BspEI BspHI BsrFI BssHII BssSI
BstBI BstEII BtgZI ClaI CspCI DrdI EagI EcoICRI
EcoRV FseI FspI FspAI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I
XcmI XhoI XmaI ZraI