Restriction map for
pF1KE1085
(Linear) (Six-base) MAPSORT of: KIEE1085.seq Check: 4477 from: 1 to: 2553
KIEE1085.orf
With 182 enzymes: SgfI *
July 28, 2009 15:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 69 603 2553
Size: 69 534 1950
Fragments arranged by size:
1950 534 69
AatII G_ACGT'C
Cuts at: 0 247 2553
Size: 247 2306
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2152 2158 2553
Size: 2152 6 395
Fragments arranged by size:
2152 395 6
AflII C'TTAA_G
Cuts at: 0 688 2553
Size: 688 1865
AflIII A'CryG_T
Cuts at: 0 180 2553
Size: 180 2373
AgeI A'CCGG_T
Cuts at: 0 528 2553
Size: 528 2025
AleI CACnn'nnGTG
Cuts at: 0 529 1864 2553
Size: 529 1335 689
Fragments arranged by size:
1335 689 529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 38 2553
Size: 38 2515
AlwNI CAG_nnn'CTG
Cuts at: 0 12 906 1039 1068 2055 2553
Size: 12 894 133 29 987 498
Fragments arranged by size:
987 894 498 133 29 12
ApaI G_GGCC'C
Cuts at: 0 1062 1098 1424 2553
Size: 1062 36 326 1129
Fragments arranged by size:
1129 1062 326 36
AvaI C'yCGr_G
Cuts at: 0 403 1287 1694 1912 2553
Size: 403 884 407 218 641
Fragments arranged by size:
884 641 407 403 218
BanI G'GyrC_C
Cuts at: 0 531 548 580 802 1042 1411 1928 2553
Size: 531 17 32 222 240 369 517 625
Fragments arranged by size:
625 531 517 369 240 222 32 17
BanII G_rGCy'C
Cuts at: 0 214 439 743 1062 1098 1126 1355 1370
Size: 214 225 304 319 36 28 229 15
Cuts at: 1370 1424 2530 2553
Size: 54 1106 23
Fragments arranged by size:
1106 319 304 229 225 214 54 36
28 23 15
BbeI G_GCGC'C
Cuts at: 0 1046 2553
Size: 1046 1507
BbsI GAAGACnn'nnnn_
Cuts at: 0 1268 2146 2553
Size: 1268 878 407
Fragments arranged by size:
1268 878 407
BbvCI CC'TCA_GC
Cuts at: 0 1090 1721 2553
Size: 1090 631 832
Fragments arranged by size:
1090 832 631
BciVI GTATCCnnnnn_n'
Cuts at: 0 975 2553
Size: 975 1578
BglI GCCn_nnn'nGGC
Cuts at: 0 928 1113 1374 1795 2209 2275 2284 2553
Size: 928 185 261 421 414 66 9 269
Fragments arranged by size:
928 421 414 269 261 185 66 9
BlpI GC'TnA_GC
Cuts at: 0 1768 2553
Size: 1768 785
Bme1580I G_kGCm'C
Cuts at: 0 551 585 805 1062 1098 1168 1175 1343
Size: 551 34 220 257 36 70 7 168
Cuts at: 1343 1414 1424 2384 2553
Size: 71 10 960 169
Fragments arranged by size:
960 551 257 220 169 168 71 70
36 34 10 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 443 533 769 1162 2553
Size: 443 90 236 393 1391
Fragments arranged by size:
1391 443 393 236 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1320 1578 2441 2553
Size: 1320 258 863 112
Fragments arranged by size:
1320 863 258 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 619 625 640 805 895 1090 1480
Size: 361 258 6 15 165 90 195 390
Cuts at: 1480 1527 2553
Size: 47 1026
Fragments arranged by size:
1026 390 361 258 195 165 90 47
15 6
Bpu10I CC'TnA_GC
Cuts at: 0 208 917 1090 1639 1721 2553
Size: 208 709 173 549 82 832
Fragments arranged by size:
832 709 549 208 173 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 871 2553
Size: 124 747 1682
Fragments arranged by size:
1682 747 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 337 781 871 2553
Size: 97 240 444 90 1682
Fragments arranged by size:
1682 444 240 97 90
BsaAI yAC'GTr
Cuts at: 0 18 147 561 1974 2553
Size: 18 129 414 1413 579
Fragments arranged by size:
1413 579 414 129 18
BsaBI GATnn'nnATC
Cuts at: 0 1216 2553
Size: 1216 1337
BsaHI Gr'CG_yC
Cuts at: 0 244 1043 2553
Size: 244 799 1510
Fragments arranged by size:
1510 799 244
BsaWI w'CCGG_w
Cuts at: 0 528 1484 2553
Size: 528 956 1069
Fragments arranged by size:
1069 956 528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1147 1177 2553
Size: 1147 30 1376
Fragments arranged by size:
1376 1147 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1414 1474 1503 1762 1765 2553
Size: 622 792 60 29 259 3 788
Fragments arranged by size:
792 788 622 259 60 29 3
BseYI C'CCAG_C
Cuts at: 0 48 881 938 1634 2383 2553
Size: 48 833 57 696 749 170
Fragments arranged by size:
833 749 696 170 57 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1996 2068 2553
Size: 211 1785 72 485
Fragments arranged by size:
1785 485 211 72
BsiEI CG_ry'CG
Cuts at: 0 312 458 2553
Size: 312 146 2095
Fragments arranged by size:
2095 312 146
BsiHKAI G_wGCw'C
Cuts at: 0 214 265 339 635 656 743 1330 2345
Size: 214 51 74 296 21 87 587 1015
Cuts at: 2345 2422 2500 2553
Size: 77 78 53
Fragments arranged by size:
1015 587 296 214 87 78 77 74
53 51 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 406 2553
Size: 406 2147
Bsp1286I G_dGCh'C
Cuts at: 0 214 265 339 439 551 585 635 656
Size: 214 51 74 100 112 34 50 21
Cuts at: 656 743 805 1062 1098 1126 1168 1175 1330
Size: 87 62 257 36 28 42 7 155
Cuts at: 1330 1343 1355 1370 1414 1424 2345 2384 2422
Size: 13 12 15 44 10 921 39 38
Cuts at: 2422 2500 2530 2553
Size: 78 30 23
Fragments arranged by size:
921 257 214 155 112 100 87 78
74 62 51 50 44 42 39 38
36 34 30 28 23 21 15 13
12 10 7
BspHI T'CATG_A
Cuts at: 0 1445 2553
Size: 1445 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 69 603 2553
Size: 69 534 1950
Fragments arranged by size:
1950 534 69
BsrBI CCG'CTC
Cuts at: 0 1839 2553
Size: 1839 714
BsrDI GCAATG_nn'
Cuts at: 0 1109 2553
Size: 1109 1444
BsrFI r'CCGG_y
Cuts at: 0 427 457 528 1414 1673 2553
Size: 427 30 71 886 259 880
Fragments arranged by size:
886 880 427 259 71 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 906 2055 2553
Size: 906 1149 498
Fragments arranged by size:
1149 906 498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 2553
Size: 432 2121
BtgI C'CryG_G
Cuts at: 0 98 222 312 348 364 505 1006 1475
Size: 98 124 90 36 16 141 501 469
Cuts at: 1475 2042 2244 2318 2553
Size: 567 202 74 235
Fragments arranged by size:
567 501 469 235 202 141 124 98
90 74 36 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1250 1968 2426 2553
Size: 1250 718 458 127
Fragments arranged by size:
1250 718 458 127
BtsI GCAGTG_nn'
Cuts at: 0 194 1715 2553
Size: 194 1521 838
Fragments arranged by size:
1521 838 194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 255 290 2553
Size: 255 35 2263
Fragments arranged by size:
2263 255 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 970 1975 2068 2553
Size: 970 1005 93 485
Fragments arranged by size:
1005 970 485 93
EaeI y'GGCC_r
Cuts at: 0 309 416 455 952 1025 1622 1661 2129
Size: 309 107 39 497 73 597 39 468
Cuts at: 2129 2234 2346 2375 2553
Size: 105 112 29 178
Fragments arranged by size:
597 497 468 309 178 112 107 105
73 39 39 29
EagI C'GGCC_G
Cuts at: 0 309 455 2553
Size: 309 146 2098
Fragments arranged by size:
2098 309 146
EarI CTCTTCn'nnn_
Cuts at: 0 1318 2179 2543 2553
Size: 1318 861 364 10
Fragments arranged by size:
1318 861 364 10
EcoICRI GAG'CTC
Cuts at: 0 212 741 2553
Size: 212 529 1812
Fragments arranged by size:
1812 529 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 619 625 640 805 895 1090 1480
Size: 361 258 6 15 165 90 195 390
Cuts at: 1480 1527 2152 2158 2553
Size: 47 625 6 395
Fragments arranged by size:
625 395 390 361 258 195 165 90
47 15 6 6
EcoO109I rG'GnC_Cy
Cuts at: 0 9 38 78 771 1094 1095 1421 1590
Size: 9 29 40 693 323 1 326 169
Cuts at: 1590 2553
Size: 963
Fragments arranged by size:
963 693 326 323 169 40 29 9
1
HaeII r_GCGC'y
Cuts at: 0 198 898 1046 2553
Size: 198 700 148 1507
Fragments arranged by size:
1507 700 198 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 1714 1746 1768 1800 2299 2331
Size: 1516 32 166 32 22 32 499 32
Cuts at: 2331 2449 2481 2553
Size: 118 32 72
Fragments arranged by size:
1516 499 166 118 72 32 32 32
32 32 22
HincII GTy'rAC
Cuts at: 0 1512 1980 2295 2553
Size: 1512 468 315 258
Fragments arranged by size:
1512 468 315 258
KasI G'GCGC_C
Cuts at: 0 1042 2553
Size: 1042 1511
MluI A'CGCG_T
Cuts at: 0 180 2553
Size: 180 2373
MscI TGG'CCA
Cuts at: 0 954 1027 2131 2553
Size: 954 73 1104 422
Fragments arranged by size:
1104 954 422 73
MslI CAynn'nnrTG
Cuts at: 0 529 1864 2553
Size: 529 1335 689
Fragments arranged by size:
1335 689 529
MspA1I CmG'CkG
Cuts at: 0 350 366 603 721 1039 1056 1177 1660
Size: 350 16 237 118 318 17 121 483
Cuts at: 1660 1689 2055 2066 2387 2553
Size: 29 366 11 321 166
Fragments arranged by size:
483 366 350 321 318 237 166 121
118 29 17 16 11
NaeI GCC'GGC
Cuts at: 0 459 1675 2553
Size: 459 1216 878
Fragments arranged by size:
1216 878 459
NarI GG'CG_CC
Cuts at: 0 1043 2553
Size: 1043 1510
NdeI CA'TA_TG
Cuts at: 0 1199 2553
Size: 1199 1354
NgoMIV G'CCGG_C
Cuts at: 0 457 1673 2553
Size: 457 1216 880
Fragments arranged by size:
1216 880 457
NruI TCG'CGA
Cuts at: 0 2411 2553
Size: 2411 142
NspI r_CATG'y
Cuts at: 0 834 2553
Size: 834 1719
PasI CC'CwG_GG
Cuts at: 0 34 2553
Size: 34 2519
PflMI CCAn_nnn'nTGG
Cuts at: 0 1068 1149 1503 2553
Size: 1068 81 354 1050
Fragments arranged by size:
1068 1050 354 81
PfoI T'CCnGG_A
Cuts at: 0 656 2553
Size: 656 1897
PmlI CAC'GTG
Cuts at: 0 18 561 2553
Size: 18 543 1992
Fragments arranged by size:
1992 543 18
PpuMI rG'GwC_Cy
Cuts at: 0 9 38 78 771 2553
Size: 9 29 40 693 1782
Fragments arranged by size:
1782 693 40 29 9
PspOMI G'GGCC_C
Cuts at: 0 1058 1094 1420 2553
Size: 1058 36 326 1133
Fragments arranged by size:
1133 1058 326 36
PstI C_TGCA'G
Cuts at: 0 902 911 1182 1650 1874 2553
Size: 902 9 271 468 224 679
Fragments arranged by size:
902 679 468 271 224 9
PvuII CAG'CTG
Cuts at: 0 603 1689 2055 2387 2553
Size: 603 1086 366 332 166
Fragments arranged by size:
1086 603 366 332 166
RsrII CG'GwC_CG
Cuts at: 0 972 2553
Size: 972 1581
SacI G_AGCT'C
Cuts at: 0 214 743 2553
Size: 214 529 1810
Fragments arranged by size:
1810 529 214
SacII CC_GC'GG
Cuts at: 0 351 367 2553
Size: 351 16 2186
Fragments arranged by size:
2186 351 16
SanDI GG'GwC_CC
Cuts at: 0 38 2553
Size: 38 2515
SapI GCTCTTCn'nnn_
Cuts at: 0 1318 2179 2553
Size: 1318 861 374
Fragments arranged by size:
1318 861 374
SbfI CC_TGCA'GG
Cuts at: 0 1650 2553
Size: 1650 903
SexAI A'CCwGG_T
Cuts at: 0 1931 2553
Size: 1931 622
SfcI C'TryA_G
Cuts at: 0 898 907 1178 1646 1870 2103 2553
Size: 898 9 271 468 224 233 450
Fragments arranged by size:
898 468 450 271 233 224 9
SfoI GGC'GCC
Cuts at: 0 1044 2553
Size: 1044 1509
SgrAI Cr'CCGG_yG
Cuts at: 0 528 1414 2553
Size: 528 886 1139
Fragments arranged by size:
1139 886 528
SmaI CCC'GGG
Cuts at: 0 1289 2553
Size: 1289 1264
SmlI C'TyrA_G
Cuts at: 0 139 403 688 886 1694 2553
Size: 139 264 285 198 808 859
Fragments arranged by size:
859 808 285 264 198 139
SphI G_CATG'C
Cuts at: 0 834 2553
Size: 834 1719
StuI AGG'CCT
Cuts at: 0 949 1116 1250 1644 2553
Size: 949 167 134 394 909
Fragments arranged by size:
949 909 394 167 134
StyI C'CwwG_G
Cuts at: 0 74 860 2438 2553
Size: 74 786 1578 115
Fragments arranged by size:
1578 786 115 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 1360 1372 2553
Size: 438 922 12 1181
Fragments arranged by size:
1181 922 438 12
TatI w'GTAC_w
Cuts at: 0 681 2553
Size: 681 1872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 1118 1147 1150 1179 2553
Size: 50 32 1036 29 3 29 1374
Fragments arranged by size:
1374 1036 50 32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 0 2260 2553
Size: 2260 293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2040 2553
Size: 2040 513
XhoI C'TCGA_G
Cuts at: 0 403 1694 2553
Size: 403 1291 859
Fragments arranged by size:
1291 859 403
XmaI C'CCGG_G
Cuts at: 0 1287 2553
Size: 1287 1266
ZraI GAC'GTC
Cuts at: 0 245 2553
Size: 245 2308
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AgeI AleI AlfI
AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI
BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII KasI MluI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NruI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SanDI SapI SbfI SexAI SfcI SfoI SgrAI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AhdI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BclI
BfrBI BglII BmgBI BmtI BsiWI BsmI BspEI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI
DraI DraIII EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MmeI NcoI
NheI NotI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI SalI ScaI SfiI SgfI
SnaBI SpeI SrfI SspI SwaI XbaI XmnI