Restriction map for
pF1KE1079
(Linear) (Six-base) MAPSORT of: KIEE1079.seq Check: 232 from: 1 to: 3216
KIEE1079.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 0 2391 3216
Size: 2391 825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2151 2209 2879 3216
Size: 2151 58 670 337
Fragments arranged by size:
2151 670 337 58
AfeI AGC'GCT
Cuts at: 0 135 3216
Size: 135 3081
AflIII A'CryG_T
Cuts at: 0 195 425 2349 3216
Size: 195 230 1924 867
Fragments arranged by size:
1924 867 230 195
AleI CACnn'nnGTG
Cuts at: 0 1265 3216
Size: 1265 1951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 3216
Size: 320 2896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2665 2697 3216
Size: 2665 32 519
Fragments arranged by size:
2665 519 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2623 3216
Size: 2623 593
ApoI r'AATT_y
Cuts at: 0 13 535 695 1216 2268 2296 2473 2499
Size: 13 522 160 521 1052 28 177 26
Cuts at: 2499 3216
Size: 717
Fragments arranged by size:
1052 717 522 521 177 160 28 26
13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 414 447 3216
Size: 414 33 2769
Fragments arranged by size:
2769 414 33
BamHI G'GATC_C
Cuts at: 0 1860 3216
Size: 1860 1356
BanI G'GyrC_C
Cuts at: 0 3031 3216
Size: 3031 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 1451 2366 2579 3216
Size: 241 1210 915 213 637
Fragments arranged by size:
1210 915 637 241 213
BciVI GTATCCnnnnn_n'
Cuts at: 0 3129 3216
Size: 3129 87
BclI T'GATC_A
Cuts at: 0 1188 1302 2070 3216
Size: 1188 114 768 1146
Fragments arranged by size:
1188 1146 768 114
BfrBI ATG'CAT
Cuts at: 0 1004 3043 3216
Size: 1004 2039 173
Fragments arranged by size:
2039 1004 173
BglII A'GATC_T
Cuts at: 0 2713 3216
Size: 2713 503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1604 3216
Size: 1604 1612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1242 1272 2203 2233 3216
Size: 1242 30 931 30 983
Fragments arranged by size:
1242 983 931 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2618 2991 3216
Size: 2618 373 225
Fragments arranged by size:
2618 373 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 3216
Size: 1565 1651
BsiHKAI G_wGCw'C
Cuts at: 0 1344 3216
Size: 1344 1872
BsmI GAATG_Cn'
Cuts at: 0 759 1004 2473 3216
Size: 759 245 1469 743
Fragments arranged by size:
1469 759 743 245
Bsp1286I G_dGCh'C
Cuts at: 0 1344 3216
Size: 1344 1872
BspHI T'CATG_A
Cuts at: 0 2292 3216
Size: 2292 924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1077 3216
Size: 1077 2139
BsrGI T'GTAC_A
Cuts at: 0 138 2352 3216
Size: 138 2214 864
Fragments arranged by size:
2214 864 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1010 3216
Size: 1010 2206
BstYI r'GATC_y
Cuts at: 0 516 1860 2713 3216
Size: 516 1344 853 503
Fragments arranged by size:
1344 853 516 503
BtsI GCAGTG_nn'
Cuts at: 0 2073 3195 3216
Size: 2073 1122 21
Fragments arranged by size:
2073 1122 21
ClaI AT'CG_AT
Cuts at: 0 2114 3216
Size: 2114 1102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1013 1048 2747 2782 3216
Size: 1013 35 1699 35 434
Fragments arranged by size:
1699 1013 434 35 35
DraI TTT'AAA
Cuts at: 0 606 1169 1494 2853 3216
Size: 606 563 325 1359 363
Fragments arranged by size:
1359 606 563 363 325
DraIII CAC_nnn'GTG
Cuts at: 0 405 3216
Size: 405 2811
DrdI GACnn_nn'nnGTC
Cuts at: 0 200 1811 3216
Size: 200 1611 1405
Fragments arranged by size:
1611 1405 200
EaeI y'GGCC_r
Cuts at: 0 681 1879 3216
Size: 681 1198 1337
Fragments arranged by size:
1337 1198 681
EarI CTCTTCn'nnn_
Cuts at: 0 2545 3216
Size: 2545 671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1604 2151 2209 2879 3216
Size: 1604 547 58 670 337
Fragments arranged by size:
1604 670 547 337 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 86 2005 3216
Size: 86 1919 1211
Fragments arranged by size:
1919 1211 86
EcoRI G'AATT_C
Cuts at: 0 2296 3216
Size: 2296 920
EcoRV GAT'ATC
Cuts at: 0 2139 3216
Size: 2139 1077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2909 3216
Size: 2909 307
HaeII r_GCGC'y
Cuts at: 0 137 3216
Size: 137 3079
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1194 1226 1242 1274 1602 1634
Size: 979 32 183 32 16 32 328 32
Cuts at: 1634 2203 2220 2235 2252 2411 2443 3216
Size: 569 17 15 17 159 32 773
Fragments arranged by size:
979 773 569 328 183 159 32 32
32 32 32 17 17 16 15
HindIII A'AGCT_T
Cuts at: 0 174 3216
Size: 174 3042
MfeI C'AATT_G
Cuts at: 0 154 734 3216
Size: 154 580 2482
Fragments arranged by size:
2482 580 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 279 362 566 1246 1726 3216
Size: 192 87 83 204 680 480 1490
Fragments arranged by size:
1490 680 480 204 192 87 83
MscI TGG'CCA
Cuts at: 0 683 1881 3216
Size: 683 1198 1335
Fragments arranged by size:
1335 1198 683
MslI CAynn'nnrTG
Cuts at: 0 902 1265 2354 2927 3216
Size: 902 363 1089 573 289
Fragments arranged by size:
1089 902 573 363 289
MspA1I CmG'CkG
Cuts at: 0 119 1097 1712 3216
Size: 119 978 615 1504
Fragments arranged by size:
1504 978 615 119
NdeI CA'TA_TG
Cuts at: 0 2924 3216
Size: 2924 292
NsiI A_TGCA'T
Cuts at: 0 1006 3045 3216
Size: 1006 2039 171
Fragments arranged by size:
2039 1006 171
NspI r_CATG'y
Cuts at: 0 199 301 429 2353 3216
Size: 199 102 128 1924 863
Fragments arranged by size:
1924 863 199 128 102
PciI A'CATG_T
Cuts at: 0 195 425 2349 3216
Size: 195 230 1924 867
Fragments arranged by size:
1924 867 230 195
PfoI T'CCnGG_A
Cuts at: 0 1620 3216
Size: 1620 1596
PvuII CAG'CTG
Cuts at: 0 119 1097 1712 3216
Size: 119 978 615 1504
Fragments arranged by size:
1504 978 615 119
SexAI A'CCwGG_T
Cuts at: 0 27 3216
Size: 27 3189
SfcI C'TryA_G
Cuts at: 0 657 3216
Size: 657 2559
SphI G_CATG'C
Cuts at: 0 301 3216
Size: 301 2915
SspI AAT'ATT
Cuts at: 0 1233 3216
Size: 1233 1983
StuI AGG'CCT
Cuts at: 0 2000 3216
Size: 2000 1216
StyI C'CwwG_G
Cuts at: 0 2402 3216
Size: 2402 814
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 787 1568 2318 2690 2711 3216
Size: 787 781 750 372 21 505
Fragments arranged by size:
787 781 750 505 372 21
TatI w'GTAC_w
Cuts at: 0 138 2352 2887 2917 3216
Size: 138 2214 535 30 299
Fragments arranged by size:
2214 535 299 138 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 3216
Size: 15 32 3169
Fragments arranged by size:
3169 32 15
Tth111I GACn'n_nGTC
Cuts at: 0 2049 3216
Size: 2049 1167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2579 3216
Size: 2579 637
XmnI GAAnn'nnTTC
Cuts at: 0 2951 3216
Size: 2951 265
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApoI BaeI BamHI BanI BbsI BciVI BclI BfrBI
BglII BpmI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstAPI BstYI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoRI
EcoRV FalI HaeII Hin4I HindIII MfeI MmeI MscI
MslI MspA1I NdeI NsiI NspI PciI PfoI PvuII
SexAI SfcI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BanII BbeI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI EagI EciI EcoICRI EcoO109I FseI FspI FspAI
HincII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI XbaI XhoI XmaI ZraI