Restriction map for
pF1KE1077
(Linear) (Six-base) MAPSORT of: KIEE1077.seq Check: 2293 from: 1 to: 684
KIEE1077.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 373 555 684
Size: 190 183 182 129
Fragments arranged by size:
190 183 182 129
AfeI AGC'GCT
Cuts at: 0 207 667 684
Size: 207 460 17
Fragments arranged by size:
460 207 17
AflIII A'CryG_T
Cuts at: 0 82 135 684
Size: 82 53 549
Fragments arranged by size:
549 82 53
AgeI A'CCGG_T
Cuts at: 0 596 684
Size: 596 88
AleI CACnn'nnGTG
Cuts at: 0 616 684
Size: 616 68
ApoI r'AATT_y
Cuts at: 0 288 684
Size: 288 396
AvaI C'yCGr_G
Cuts at: 0 238 684
Size: 238 446
AvrII C'CTAG_G
Cuts at: 0 69 684
Size: 69 615
BanI G'GyrC_C
Cuts at: 0 123 213 593 684
Size: 123 90 380 91
Fragments arranged by size:
380 123 91 90
BanII G_rGCy'C
Cuts at: 0 239 684
Size: 239 445
BciVI GTATCCnnnnn_n'
Cuts at: 0 492 684
Size: 492 192
BclI T'GATC_A
Cuts at: 0 453 684
Size: 453 231
BfrBI ATG'CAT
Cuts at: 0 382 684
Size: 382 302
Bme1580I G_kGCm'C
Cuts at: 0 128 684
Size: 128 556
BmgBI CAC'GTC
Cuts at: 0 138 684
Size: 138 546
BsaI GGTCTCn'nnnn_
Cuts at: 0 539 684
Size: 539 145
BsaAI yAC'GTr
Cuts at: 0 83 318 684
Size: 83 235 366
Fragments arranged by size:
366 235 83
BsaHI Gr'CG_yC
Cuts at: 0 221 684
Size: 221 463
BsaWI w'CCGG_w
Cuts at: 0 308 596 684
Size: 308 288 88
Fragments arranged by size:
308 288 88
BsiHKAI G_wGCw'C
Cuts at: 0 239 259 581 684
Size: 239 20 322 103
Fragments arranged by size:
322 239 103 20
Bsp1286I G_dGCh'C
Cuts at: 0 128 239 259 581 684
Size: 128 111 20 322 103
Fragments arranged by size:
322 128 111 103 20
BsrBI CCG'CTC
Cuts at: 0 243 684
Size: 243 441
BsrFI r'CCGG_y
Cuts at: 0 48 114 590 596 684
Size: 48 66 476 6 88
Fragments arranged by size:
476 88 66 48 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 684
Size: 510 174
BstYI r'GATC_y
Cuts at: 0 305 684
Size: 305 379
BtgI C'CryG_G
Cuts at: 0 230 273 684
Size: 230 43 411
Fragments arranged by size:
411 230 43
BtsI GCAGTG_nn'
Cuts at: 0 474 684
Size: 474 210
EarI CTCTTCn'nnn_
Cuts at: 0 392 632 684
Size: 392 240 52
Fragments arranged by size:
392 240 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 684
Size: 70 614
EcoICRI GAG'CTC
Cuts at: 0 237 684
Size: 237 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 373 555 684
Size: 190 183 182 129
Fragments arranged by size:
190 183 182 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 684
Size: 309 375
FspI TGC'GCA
Cuts at: 0 35 684
Size: 35 649
HaeII r_GCGC'y
Cuts at: 0 209 566 591 669 684
Size: 209 357 25 78 15
Fragments arranged by size:
357 209 78 25 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 566 598 684
Size: 490 32 44 32 86
Fragments arranged by size:
490 86 44 32 32
MslI CAynn'nnrTG
Cuts at: 0 616 684
Size: 616 68
MspA1I CmG'CkG
Cuts at: 0 15 39 684
Size: 15 24 645
Fragments arranged by size:
645 24 15
NaeI GCC'GGC
Cuts at: 0 50 592 684
Size: 50 542 92
Fragments arranged by size:
542 92 50
NcoI C'CATG_G
Cuts at: 0 273 684
Size: 273 411
NgoMIV G'CCGG_C
Cuts at: 0 48 590 684
Size: 48 542 94
Fragments arranged by size:
542 94 48
NsiI A_TGCA'T
Cuts at: 0 384 684
Size: 384 300
NspI r_CATG'y
Cuts at: 0 155 684
Size: 155 529
PasI CC'CwG_GG
Cuts at: 0 539 684
Size: 539 145
PmlI CAC'GTG
Cuts at: 0 83 318 684
Size: 83 235 366
Fragments arranged by size:
366 235 83
PspXI vC'TCGA_Gb
Cuts at: 0 238 684
Size: 238 446
PstI C_TGCA'G
Cuts at: 0 161 684
Size: 161 523
PvuII CAG'CTG
Cuts at: 0 39 684
Size: 39 645
SacI G_AGCT'C
Cuts at: 0 239 684
Size: 239 445
SbfI CC_TGCA'GG
Cuts at: 0 161 684
Size: 161 523
SfcI C'TryA_G
Cuts at: 0 157 684
Size: 157 527
SmlI C'TyrA_G
Cuts at: 0 238 684
Size: 238 446
SphI G_CATG'C
Cuts at: 0 155 684
Size: 155 529
StyI C'CwwG_G
Cuts at: 0 69 273 394 684
Size: 69 204 121 290
Fragments arranged by size:
290 204 121 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 684
Size: 206 32 446
Fragments arranged by size:
446 206 32
XhoI C'TCGA_G
Cuts at: 0 238 684
Size: 238 446
XmnI GAAnn'nnTTC
Cuts at: 0 292 684
Size: 292 392
Enzymes that do cut:
AcuI AfeI AflIII AgeI AleI ApoI AvaI AvrII
BanI BanII BciVI BclI BfrBI Bme1580I BmgBI BsaI
BsaAI BsaHI BsaWI BsiHKAI Bsp1286I BsrBI BsrFI BstXI
BstYI BtgI BtsI EarI EciI EcoICRI Eco57MI FalI
FspI HaeII Hin4I MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PmlI PspXI PstI PvuII SacI
SbfI SfcI SmlI SphI StyI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BbvCI BcgI BglI BglII BlpI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaXI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoNI EcoO109I EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI PacI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XmaI ZraI