Restriction map for
pF1KE1076
(Linear) (Six-base) MAPSORT of: KIEE1076.seq Check: 5612 from: 1 to: 888
KIEE1076.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 721 888
Size: 721 167
AleI CACnn'nnGTG
Cuts at: 0 856 888
Size: 856 32
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 888
Size: 229 32 627
Fragments arranged by size:
627 229 32
ApaI G_GGCC'C
Cuts at: 0 403 888
Size: 403 485
ApoI r'AATT_y
Cuts at: 0 567 760 888
Size: 567 193 128
Fragments arranged by size:
567 193 128
BanI G'GyrC_C
Cuts at: 0 12 39 888
Size: 12 27 849
Fragments arranged by size:
849 27 12
BanII G_rGCy'C
Cuts at: 0 403 888
Size: 403 485
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 888
Size: 167 721
Bme1580I G_kGCm'C
Cuts at: 0 403 888
Size: 403 485
BmgBI CAC'GTC
Cuts at: 0 377 888
Size: 377 511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 830 888
Size: 207 623 58
Fragments arranged by size:
623 207 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 841 888
Size: 757 84 47
Fragments arranged by size:
757 84 47
BsiHKAI G_wGCw'C
Cuts at: 0 628 888
Size: 628 260
Bsp1286I G_dGCh'C
Cuts at: 0 403 628 888
Size: 403 225 260
Fragments arranged by size:
403 260 225
BsrBI CCG'CTC
Cuts at: 0 132 888
Size: 132 756
BsrDI GCAATG_nn'
Cuts at: 0 692 888
Size: 692 196
BsrGI T'GTAC_A
Cuts at: 0 556 888
Size: 556 332
BssSI C'ACGA_G
Cuts at: 0 28 112 888
Size: 28 84 776
Fragments arranged by size:
776 84 28
BstEII G'GTnAC_C
Cuts at: 0 166 888
Size: 166 722
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 888
Size: 255 633
BstYI r'GATC_y
Cuts at: 0 200 244 888
Size: 200 44 644
Fragments arranged by size:
644 200 44
Bsu36I CC'TnA_GG
Cuts at: 0 824 888
Size: 824 64
BtgI C'CryG_G
Cuts at: 0 138 888
Size: 138 750
DrdI GACnn_nn'nnGTC
Cuts at: 0 463 888
Size: 463 425
EaeI y'GGCC_r
Cuts at: 0 296 694 888
Size: 296 398 194
Fragments arranged by size:
398 296 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 830 888
Size: 207 623 58
Fragments arranged by size:
623 207 58
EcoO109I rG'GnC_Cy
Cuts at: 0 400 888
Size: 400 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 707 739 803 835 888
Size: 496 32 179 32 64 32 53
Fragments arranged by size:
496 179 64 53 32 32 32
MfeI C'AATT_G
Cuts at: 0 492 888
Size: 492 396
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 888
Size: 567 321
MscI TGG'CCA
Cuts at: 0 298 888
Size: 298 590
MslI CAynn'nnrTG
Cuts at: 0 226 253 280 856 888
Size: 226 27 27 576 32
Fragments arranged by size:
576 226 32 27 27
MspA1I CmG'CkG
Cuts at: 0 786 888
Size: 786 102
PspOMI G'GGCC_C
Cuts at: 0 399 888
Size: 399 489
ScaI AGT'ACT
Cuts at: 0 418 888
Size: 418 470
StyI C'CwwG_G
Cuts at: 0 669 888
Size: 669 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 888
Size: 166 722
TatI w'GTAC_w
Cuts at: 0 416 556 888
Size: 416 140 332
Fragments arranged by size:
416 332 140
Enzymes that do cut:
AhdI AleI AloI ApaI ApoI BanI BanII BbsI
Bme1580I BmgBI BpmI BseRI BsiHKAI Bsp1286I BsrBI BsrDI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI DrdI
EaeI Eco57MI EcoO109I Hin4I MfeI MmeI MscI MslI
MspA1I PspOMI ScaI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrFI BssHII BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI