(Linear) (Six-base) MAPSORT of: KIEE1069.seq  Check: 4607  from: 1  to: 531



KIEE1069.orf

 With 182 enzymes: SgfI * 

                        February 15, 2010 17:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     531 
   Size:         14     517
 
AvaI C'yCGr_G

Cuts at:      0     141     236     531 
   Size:        141      95     295

  Fragments arranged by size: 

                 295     141      95
 
BanI G'GyrC_C

Cuts at:      0     203     421     531 
   Size:        203     218     110

  Fragments arranged by size: 

                 218     203     110
 
BbeI G_GCGC'C

Cuts at:      0     425     531 
   Size:        425     106
 
BlpI GC'TnA_GC

Cuts at:      0     454     531 
   Size:        454      77
 
Bme1580I G_kGCm'C

Cuts at:      0     206     531 
   Size:        206     325
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      63     328     531 
   Size:         63     265     203

  Fragments arranged by size: 

                 265     203      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     229     531 
   Size:        178      51     302

  Fragments arranged by size: 

                 302     178      51
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     531 
   Size:        231     300
 
BsaHI Gr'CG_yC

Cuts at:      0     256     422     531 
   Size:        256     166     109

  Fragments arranged by size: 

                 256     166     109
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     531 
   Size:         76     455
 
BseYI C'CCAG_C

Cuts at:      0     432     531 
   Size:        432      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     531 
   Size:         34     497
 
BsmI GAATG_Cn'

Cuts at:      0      55     531 
   Size:         55     476
 
Bsp1286I G_dGCh'C

Cuts at:      0     206     531 
   Size:        206     325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     531 
   Size:         74     457
 
BstYI r'GATC_y

Cuts at:      0     153     531 
   Size:        153     378
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     249     531 
   Size:        249     282
 
BtsI GCAGTG_nn'

Cuts at:      0     217     531 
   Size:        217     314
 
DraIII CAC_nnn'GTG

Cuts at:      0      12     531 
   Size:         12     519
 
EaeI y'GGCC_r

Cuts at:      0     320     497     531 
   Size:        320     177      34

  Fragments arranged by size: 

                 320     177      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      39     531 
   Size:         39     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     178     229     531 
   Size:         14     164      51     302

  Fragments arranged by size: 

                 302     164      51      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      91     531 
   Size:         91     440
 
EcoO109I rG'GnC_Cy

Cuts at:      0     145     174     213     531 
   Size:        145      29      39     318

  Fragments arranged by size: 

                 318     145      39      29
 
HaeII r_GCGC'y

Cuts at:      0     425     531 
   Size:        425     106
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     531 
   Size:        108      32     391

  Fragments arranged by size: 

                 391     108      32
 
HincII GTy'rAC

Cuts at:      0     510     531 
   Size:        510      21
 
KasI G'GCGC_C

Cuts at:      0     421     531 
   Size:        421     110
 
MslI CAynn'nnrTG

Cuts at:      0     472     490     531 
   Size:        472      18      41

  Fragments arranged by size: 

                 472      41      18
 
NarI GG'CG_CC

Cuts at:      0     422     531 
   Size:        422     109
 
NspI r_CATG'y

Cuts at:      0     402     531 
   Size:        402     129
 
PfoI T'CCnGG_A

Cuts at:      0      59     531 
   Size:         59     472
 
SfoI GGC'GCC

Cuts at:      0     423     531 
   Size:        423     108
 
SmaI CCC'GGG

Cuts at:      0     238     531 
   Size:        238     293
 
SmlI C'TyrA_G

Cuts at:      0     246     531 
   Size:        246     285
 
SphI G_CATG'C

Cuts at:      0     402     531 
   Size:        402     129
 
StyI C'CwwG_G

Cuts at:      0      92     531 
   Size:         92     439
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     215     531 
   Size:        215     316
 
XmaI C'CCGG_G

Cuts at:      0     236     531 
   Size:        236     295
 

 Enzymes that do cut:

     AcuI     AvaI     BanI     BbeI     BlpI Bme1580I     BplI     BpmI
    BpuEI    BsaHI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspMI
    BstYI    BtgZI     BtsI   DraIII     EaeI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     KasI     MslI     NarI     NspI
     PfoI     SfoI     SmaI     SmlI     SphI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI     DrdI
     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI