(Linear) (Six-base) MAPSORT of: KIEE1064.seq  Check: 8728  from: 1  to: 300



KIEE1064.orf

 With 182 enzymes: SgfI * 

                        February 26, 2009 15:00  ..

AccI GT'mk_AC

Cuts at:      0       4     300 
   Size:          4     296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     300 
   Size:         28     272
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45     300 
   Size:         45     255
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     110     300 
   Size:        110     190
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     300 
   Size:        167     133
 
BsiEI CG_ry'CG

Cuts at:      0     144     300 
   Size:        144     156
 
BtsI GCAGTG_nn'

Cuts at:      0     154     300 
   Size:        154     146
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     300 
   Size:        196     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     300 
   Size:         28     272
 
HaeII r_GCGC'y

Cuts at:      0     170     300 
   Size:        170     130
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      42      74     300 
   Size:         42      32     226

  Fragments arranged by size: 

                 226      42      32
 
HincII GTy'rAC

Cuts at:      0      93     300 
   Size:         93     207
 
HpaI GTT'AAC

Cuts at:      0      93     300 
   Size:         93     207
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     300 
   Size:         37     263
 
MslI CAynn'nnrTG

Cuts at:      0     280     300 
   Size:        280      20
 
PstI C_TGCA'G

Cuts at:      0     161     300 
   Size:        161     139
 
SfcI C'TryA_G

Cuts at:      0       5     157     300 
   Size:          5     152     143

  Fragments arranged by size: 

                 152     143       5
 
SspI AAT'ATT

Cuts at:      0     211     300 
   Size:        211      89
 

 Enzymes that do cut:

     AccI     AcuI     BbsI     BsaI     BsgI    BsiEI     BtsI     EarI
  Eco57MI    HaeII    Hin4I   HincII     HpaI     MmeI     MslI     PstI
     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI