Restriction map for
pF1KE1061
(Linear) (Six-base) MAPSORT of: KIEE1061.seq Check: 7550 from: 1 to: 897
KIEE1061.orf
With 182 enzymes: SgfI *
November 27, 2008 10:48 ..
AclI AA'CG_TT
Cuts at: 0 463 897
Size: 463 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 876 897
Size: 815 61 21
Fragments arranged by size:
815 61 21
AleI CACnn'nnGTG
Cuts at: 0 154 897
Size: 154 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 116 456 897
Size: 116 340 441
Fragments arranged by size:
441 340 116
AlwNI CAG_nnn'CTG
Cuts at: 0 648 808 897
Size: 648 160 89
Fragments arranged by size:
648 160 89
ApoI r'AATT_y
Cuts at: 0 357 558 897
Size: 357 201 339
Fragments arranged by size:
357 339 201
BanII G_rGCy'C
Cuts at: 0 335 897
Size: 335 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 544 897
Size: 544 353
BbvCI CC'TCA_GC
Cuts at: 0 786 851 897
Size: 786 65 46
Fragments arranged by size:
786 65 46
BglII A'GATC_T
Cuts at: 0 117 897
Size: 117 780
BmtI G_CTAG'C
Cuts at: 0 376 460 897
Size: 376 84 437
Fragments arranged by size:
437 376 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 725 897
Size: 31 694 172
Fragments arranged by size:
694 172 31
Bpu10I CC'TnA_GC
Cuts at: 0 786 851 897
Size: 786 65 46
Fragments arranged by size:
786 65 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 396 786 840 892 897
Size: 396 390 54 52 5
Fragments arranged by size:
396 390 54 52 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 186 216 897
Size: 186 30 681
Fragments arranged by size:
681 186 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 774 897
Size: 191 583 123
Fragments arranged by size:
583 191 123
BsiHKAI G_wGCw'C
Cuts at: 0 335 897
Size: 335 562
Bsp1286I G_dGCh'C
Cuts at: 0 335 897
Size: 335 562
BspHI T'CATG_A
Cuts at: 0 632 897
Size: 632 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 41 508 897
Size: 41 467 389
Fragments arranged by size:
467 389 41
BstYI r'GATC_y
Cuts at: 0 117 488 645 708 897
Size: 117 371 157 63 189
Fragments arranged by size:
371 189 157 117 63
BtgI C'CryG_G
Cuts at: 0 34 821 897
Size: 34 787 76
Fragments arranged by size:
787 76 34
BtsI GCAGTG_nn'
Cuts at: 0 194 363 897
Size: 194 169 534
Fragments arranged by size:
534 194 169
ClaI AT'CG_AT
Cuts at: 0 531 746 897
Size: 531 215 151
Fragments arranged by size:
531 215 151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 112 147 897
Size: 112 35 750
Fragments arranged by size:
750 112 35
EaeI y'GGCC_r
Cuts at: 0 71 897
Size: 71 826
EarI CTCTTCn'nnn_
Cuts at: 0 895 897
Size: 895 2
EcoICRI GAG'CTC
Cuts at: 0 333 897
Size: 333 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 725 815 876 897
Size: 31 694 90 61 21
Fragments arranged by size:
694 90 61 31 21
EcoO109I rG'GnC_Cy
Cuts at: 0 835 897
Size: 835 62
HaeII r_GCGC'y
Cuts at: 0 704 897
Size: 704 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 513 545 802 834 897
Size: 396 32 85 32 257 32 63
Fragments arranged by size:
396 257 85 63 32 32 32
MscI TGG'CCA
Cuts at: 0 73 897
Size: 73 824
MslI CAynn'nnrTG
Cuts at: 0 39 154 438 897
Size: 39 115 284 459
Fragments arranged by size:
459 284 115 39
MspA1I CmG'CkG
Cuts at: 0 855 897
Size: 855 42
NcoI C'CATG_G
Cuts at: 0 34 897
Size: 34 863
NdeI CA'TA_TG
Cuts at: 0 473 897
Size: 473 424
NheI G'CTAG_C
Cuts at: 0 372 456 897
Size: 372 84 441
Fragments arranged by size:
441 372 84
NspI r_CATG'y
Cuts at: 0 372 605 897
Size: 372 233 292
Fragments arranged by size:
372 292 233
PstI C_TGCA'G
Cuts at: 0 210 644 845 897
Size: 210 434 201 52
Fragments arranged by size:
434 210 201 52
PvuII CAG'CTG
Cuts at: 0 855 897
Size: 855 42
SacI G_AGCT'C
Cuts at: 0 335 897
Size: 335 562
SfcI C'TryA_G
Cuts at: 0 46 206 640 841 897
Size: 46 160 434 201 56
Fragments arranged by size:
434 201 160 56 46
SpeI A'CTAG_T
Cuts at: 0 151 897
Size: 151 746
SphI G_CATG'C
Cuts at: 0 372 605 897
Size: 372 233 292
Fragments arranged by size:
372 292 233
StuI AGG'CCT
Cuts at: 0 20 897
Size: 20 877
StyI C'CwwG_G
Cuts at: 0 34 74 173 880 897
Size: 34 40 99 707 17
Fragments arranged by size:
707 99 40 34 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 186 218 659 691 897
Size: 186 32 441 32 206
Fragments arranged by size:
441 206 186 32 32
Enzymes that do cut:
AclI AcuI AleI AlfI AlwNI ApoI BanII BbsI
BbvCI BglII BmtI BpmI Bpu10I BsaI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BstXI BstYI BtgI BtsI ClaI
CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
MscI MslI MspA1I NcoI NdeI NheI NspI PstI
PvuII SacI SfcI SpeI SphI StuI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI