Restriction map for
pF1KE1054
(Linear) (Six-base) MAPSORT of: KIEE1054.seq Check: 5130 from: 1 to: 1611
KIEE1054.orf
With 182 enzymes: SgfI *
October 31, 2008 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 652 1611
Size: 652 959
AatII G_ACGT'C
Cuts at: 0 1265 1611
Size: 1265 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1611
Size: 928 683
AfeI AGC'GCT
Cuts at: 0 941 1611
Size: 941 670
AflII C'TTAA_G
Cuts at: 0 747 1360 1611
Size: 747 613 251
Fragments arranged by size:
747 613 251
AflIII A'CryG_T
Cuts at: 0 1002 1611
Size: 1002 609
AgeI A'CCGG_T
Cuts at: 0 1276 1611
Size: 1276 335
AlwNI CAG_nnn'CTG
Cuts at: 0 1498 1611
Size: 1498 113
ApoI r'AATT_y
Cuts at: 0 209 310 1611
Size: 209 101 1301
Fragments arranged by size:
1301 209 101
BanII G_rGCy'C
Cuts at: 0 745 1611
Size: 745 866
BglII A'GATC_T
Cuts at: 0 788 1611
Size: 788 823
BmgBI CAC'GTC
Cuts at: 0 101 1611
Size: 101 1510
BmrI ACTGGGnnnn_n'
Cuts at: 0 1536 1611
Size: 1536 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1484 1611
Size: 1484 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 1021 1611
Size: 96 925 590
Fragments arranged by size:
925 590 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 1611
Size: 1555 56
BsaHI Gr'CG_yC
Cuts at: 0 1262 1611
Size: 1262 349
BsaWI w'CCGG_w
Cuts at: 0 669 1276 1611
Size: 669 607 335
Fragments arranged by size:
669 607 335
BseYI C'CCAG_C
Cuts at: 0 1398 1611
Size: 1398 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1611
Size: 153 1458
BsiEI CG_ry'CG
Cuts at: 0 1281 1611
Size: 1281 330
BsmI GAATG_Cn'
Cuts at: 0 905 1611
Size: 905 706
Bsp1286I G_dGCh'C
Cuts at: 0 745 1611
Size: 745 866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 572 652 1611
Size: 356 216 80 959
Fragments arranged by size:
959 356 216 80
BsrDI GCAATG_nn'
Cuts at: 0 1422 1611
Size: 1422 189
BsrFI r'CCGG_y
Cuts at: 0 1276 1611
Size: 1276 335
BstEII G'GTnAC_C
Cuts at: 0 697 1611
Size: 697 914
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1549 1583 1611
Size: 1549 34 28
Fragments arranged by size:
1549 34 28
BstYI r'GATC_y
Cuts at: 0 125 189 788 1520 1611
Size: 125 64 599 732 91
Fragments arranged by size:
732 599 125 91 64
BtgI C'CryG_G
Cuts at: 0 32 1026 1611
Size: 32 994 585
Fragments arranged by size:
994 585 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 201 1611
Size: 201 1410
BtsI GCAGTG_nn'
Cuts at: 0 120 1611
Size: 120 1491
DrdI GACnn_nn'nnGTC
Cuts at: 0 387 1611
Size: 387 1224
EaeI y'GGCC_r
Cuts at: 0 602 1611
Size: 602 1009
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1611
Size: 817 794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 928 1021 1611
Size: 96 832 93 590
Fragments arranged by size:
832 590 96 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12 1611
Size: 12 1599
HaeII r_GCGC'y
Cuts at: 0 943 1611
Size: 943 668
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 481 497 513 1110 1142 1411 1443
Size: 465 16 16 16 597 32 269 32
Cuts at: 1443 1611
Size: 168
Fragments arranged by size:
597 465 269 168 32 32 16 16
16
HincII GTy'rAC
Cuts at: 0 837 1611
Size: 837 774
HindIII A'AGCT_T
Cuts at: 0 263 1611
Size: 263 1348
MfeI C'AATT_G
Cuts at: 0 290 576 1611
Size: 290 286 1035
Fragments arranged by size:
1035 290 286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1611
Size: 907 704
MslI CAynn'nnrTG
Cuts at: 0 450 719 1057 1611
Size: 450 269 338 554
Fragments arranged by size:
554 450 338 269
MspA1I CmG'CkG
Cuts at: 0 1041 1611
Size: 1041 570
NcoI C'CATG_G
Cuts at: 0 32 1611
Size: 32 1579
NdeI CA'TA_TG
Cuts at: 0 716 1611
Size: 716 895
NspI r_CATG'y
Cuts at: 0 455 1006 1611
Size: 455 551 605
Fragments arranged by size:
605 551 455
PciI A'CATG_T
Cuts at: 0 1002 1611
Size: 1002 609
PfoI T'CCnGG_A
Cuts at: 0 7 1611
Size: 7 1604
PstI C_TGCA'G
Cuts at: 0 353 569 1209 1611
Size: 353 216 640 402
Fragments arranged by size:
640 402 353 216
PvuII CAG'CTG
Cuts at: 0 1041 1611
Size: 1041 570
ScaI AGT'ACT
Cuts at: 0 1546 1611
Size: 1546 65
SexAI A'CCwGG_T
Cuts at: 0 701 1611
Size: 701 910
SfcI C'TryA_G
Cuts at: 0 349 565 651 1205 1611
Size: 349 216 86 554 406
Fragments arranged by size:
554 406 349 216 86
SmlI C'TyrA_G
Cuts at: 0 747 1360 1570 1611
Size: 747 613 210 41
Fragments arranged by size:
747 613 210 41
SspI AAT'ATT
Cuts at: 0 549 968 1611
Size: 549 419 643
Fragments arranged by size:
643 549 419
StyI C'CwwG_G
Cuts at: 0 32 1611
Size: 32 1579
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1284 1611
Size: 1284 327
TatI w'GTAC_w
Cuts at: 0 521 991 1544 1611
Size: 521 470 553 67
Fragments arranged by size:
553 521 470 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 944 1611
Size: 944 667
XmnI GAAnn'nnTTC
Cuts at: 0 400 1611
Size: 400 1211
ZraI GAC'GTC
Cuts at: 0 1263 1611
Size: 1263 348
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AgeI AlwNI
ApoI BanII BglII BmgBI BmrI BplI BpmI BpuEI
BsaHI BsaWI BseYI BsgI BsiEI BsmI Bsp1286I BspMI
BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI EciI Eco57MI EcoNI HaeII Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NdeI NspI
PciI PfoI PstI PvuII ScaI SexAI SfcI SmlI
SspI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AhdI AleI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmtI Bpu10I BsaI BsaAI BsaBI
BsaXI BseRI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EarI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HpaI KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XhoI XmaI