Restriction map for
pF1KE1051
(Linear) (Six-base) MAPSORT of: KIEE1051.seq Check: 8945 from: 1 to: 1335
KIEE1051.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1099 1335
Size: 1099 236
AccI GT'mk_AC
Cuts at: 0 134 1335
Size: 134 1201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1335
Size: 1312 23
AleI CACnn'nnGTG
Cuts at: 0 829 1335
Size: 829 506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1107 1335
Size: 1107 228
AlwNI CAG_nnn'CTG
Cuts at: 0 149 270 792 1335
Size: 149 121 522 543
Fragments arranged by size:
543 522 149 121
BanI G'GyrC_C
Cuts at: 0 51 161 480 711 1133 1335
Size: 51 110 319 231 422 202
Fragments arranged by size:
422 319 231 202 110 51
BanII G_rGCy'C
Cuts at: 0 351 1106 1335
Size: 351 755 229
Fragments arranged by size:
755 351 229
BbeI G_GCGC'C
Cuts at: 0 715 1335
Size: 715 620
BbsI GAAGACnn'nnnn_
Cuts at: 0 711 1335
Size: 711 624
BbvCI CC'TCA_GC
Cuts at: 0 200 1237 1335
Size: 200 1037 98
Fragments arranged by size:
1037 200 98
BglI GCCn_nnn'nGGC
Cuts at: 0 185 194 1335
Size: 185 9 1141
Fragments arranged by size:
1141 185 9
BglII A'GATC_T
Cuts at: 0 237 1335
Size: 237 1098
Bme1580I G_kGCm'C
Cuts at: 0 54 156 485 492 1136 1335
Size: 54 102 329 7 644 199
Fragments arranged by size:
644 329 199 102 54 7
BmgBI CAC'GTC
Cuts at: 0 702 1335
Size: 702 633
BmrI ACTGGGnnnn_n'
Cuts at: 0 424 479 1074 1335
Size: 424 55 595 261
Fragments arranged by size:
595 424 261 55
Bpu10I CC'TnA_GC
Cuts at: 0 200 1237 1335
Size: 200 1037 98
Fragments arranged by size:
1037 200 98
BsaAI yAC'GTr
Cuts at: 0 591 1335
Size: 591 744
BsaHI Gr'CG_yC
Cuts at: 0 8 206 712 947 1319 1335
Size: 8 198 506 235 372 16
Fragments arranged by size:
506 372 235 198 16 8
BsaWI w'CCGG_w
Cuts at: 0 291 1335
Size: 291 1044
BseYI C'CCAG_C
Cuts at: 0 71 149 301 476 1335
Size: 71 78 152 175 859
Fragments arranged by size:
859 175 152 78 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1335
Size: 337 998
BsiHKAI G_wGCw'C
Cuts at: 0 1157 1335
Size: 1157 178
BsmI GAATG_Cn'
Cuts at: 0 327 1335
Size: 327 1008
Bsp1286I G_dGCh'C
Cuts at: 0 54 156 351 485 492 1106 1136 1157
Size: 54 102 195 134 7 614 30 21
Cuts at: 1157 1335
Size: 178
Fragments arranged by size:
614 195 178 134 102 54 30 21
7
BspEI T'CCGG_A
Cuts at: 0 291 1335
Size: 291 1044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1099 1335
Size: 1099 236
BssHII G'CGCG_C
Cuts at: 0 86 1335
Size: 86 1249
BssSI C'ACGA_G
Cuts at: 0 1048 1335
Size: 1048 287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1335
Size: 492 843
BstYI r'GATC_y
Cuts at: 0 80 237 1335
Size: 80 157 1098
Fragments arranged by size:
1098 157 80
BtgI C'CryG_G
Cuts at: 0 25 192 209 431 1335
Size: 25 167 17 222 904
Fragments arranged by size:
904 222 167 25 17
BtsI GCAGTG_nn'
Cuts at: 0 883 1335
Size: 883 452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 384 419 1335
Size: 384 35 916
Fragments arranged by size:
916 384 35
EaeI y'GGCC_r
Cuts at: 0 186 428 494 509 1044 1335
Size: 186 242 66 15 535 291
Fragments arranged by size:
535 291 242 186 66 15
EarI CTCTTCn'nnn_
Cuts at: 0 970 1168 1335
Size: 970 198 167
Fragments arranged by size:
970 198 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 640 898 1335
Size: 621 19 258 437
Fragments arranged by size:
621 437 258 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1335
Size: 1312 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1335
Size: 355 980
EcoO109I rG'GnC_Cy
Cuts at: 0 34 469 984 1286 1335
Size: 34 435 515 302 49
Fragments arranged by size:
515 435 302 49 34
FspI TGC'GCA
Cuts at: 0 253 326 1335
Size: 253 73 1009
Fragments arranged by size:
1009 253 73
HaeII r_GCGC'y
Cuts at: 0 129 200 715 1335
Size: 129 71 515 620
Fragments arranged by size:
620 515 129 71
KasI G'GCGC_C
Cuts at: 0 711 1335
Size: 711 624
MscI TGG'CCA
Cuts at: 0 430 511 1335
Size: 430 81 824
Fragments arranged by size:
824 430 81
MslI CAynn'nnrTG
Cuts at: 0 436 829 865 1018 1335
Size: 436 393 36 153 317
Fragments arranged by size:
436 393 317 153 36
MspA1I CmG'CkG
Cuts at: 0 27 57 149 159 330 819 1080 1130
Size: 27 30 92 10 171 489 261 50
Cuts at: 1130 1206 1335
Size: 76 129
Fragments arranged by size:
489 261 171 129 92 76 50 30
27 10
NarI GG'CG_CC
Cuts at: 0 712 1335
Size: 712 623
NcoI C'CATG_G
Cuts at: 0 431 1335
Size: 431 904
NspI r_CATG'y
Cuts at: 0 864 876 1335
Size: 864 12 459
Fragments arranged by size:
864 459 12
PasI CC'CwG_GG
Cuts at: 0 166 952 1335
Size: 166 786 383
Fragments arranged by size:
786 383 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 1335
Size: 426 909
PfoI T'CCnGG_A
Cuts at: 0 986 1335
Size: 986 349
PmlI CAC'GTG
Cuts at: 0 591 1335
Size: 591 744
PpuMI rG'GwC_Cy
Cuts at: 0 34 469 984 1286 1335
Size: 34 435 515 302 49
Fragments arranged by size:
515 435 302 49 34
PstI C_TGCA'G
Cuts at: 0 542 1335
Size: 542 793
PvuII CAG'CTG
Cuts at: 0 330 1080 1335
Size: 330 750 255
Fragments arranged by size:
750 330 255
SacII CC_GC'GG
Cuts at: 0 28 1335
Size: 28 1307
SanDI GG'GwC_CC
Cuts at: 0 34 1335
Size: 34 1301
SapI GCTCTTCn'nnn_
Cuts at: 0 970 1335
Size: 970 365
SfcI C'TryA_G
Cuts at: 0 262 538 1188 1335
Size: 262 276 650 147
Fragments arranged by size:
650 276 262 147
SfoI GGC'GCC
Cuts at: 0 713 1335
Size: 713 622
SphI G_CATG'C
Cuts at: 0 876 1335
Size: 876 459
StyI C'CwwG_G
Cuts at: 0 431 1034 1335
Size: 431 603 301
Fragments arranged by size:
603 431 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 179 468 1335
Size: 179 289 867
Fragments arranged by size:
867 289 179
TatI w'GTAC_w
Cuts at: 0 794 1335
Size: 794 541
Tth111I GACn'n_nGTC
Cuts at: 0 1066 1279 1335
Size: 1066 213 56
Fragments arranged by size:
1066 213 56
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 1095 1335
Size: 438 657 240
Fragments arranged by size:
657 438 240
XmnI GAAnn'nnTTC
Cuts at: 0 1255 1335
Size: 1255 80
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI BanI BanII
BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI
Bpu10I BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BssHII BssSI BstXI BstYI BtgI
BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII KasI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PpuMI PstI PvuII
SacII SanDI SapI SfcI SfoI SphI StyI TaqII
TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI
BmtI BplI BpmI BpuEI BsaI BsaBI BsaXI BseRI
BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TstI XbaI XhoI XmaI ZraI