Restriction map for
pF1KE1048
(Linear) (Six-base) MAPSORT of: KIEE1048.seq Check: 3851 from: 1 to: 1191
KIEE1048.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 656 1191
Size: 656 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 353 931 1191
Size: 173 180 578 260
Fragments arranged by size:
578 260 180 173
AflII C'TTAA_G
Cuts at: 0 931 1191
Size: 931 260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 1191
Size: 193 998
ApoI r'AATT_y
Cuts at: 0 100 314 1191
Size: 100 214 877
Fragments arranged by size:
877 214 100
AseI AT'TA_AT
Cuts at: 0 263 1191
Size: 263 928
BanI G'GyrC_C
Cuts at: 0 609 1191
Size: 609 582
BbsI GAAGACnn'nnnn_
Cuts at: 0 631 1191
Size: 631 560
BfrBI ATG'CAT
Cuts at: 0 748 1191
Size: 748 443
BlpI GC'TnA_GC
Cuts at: 0 35 1191
Size: 35 1156
Bme1580I G_kGCm'C
Cuts at: 0 909 1191
Size: 909 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 969 1069 1191
Size: 969 100 122
Fragments arranged by size:
969 122 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 389 1191
Size: 389 802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 791 1191
Size: 692 99 400
Fragments arranged by size:
692 400 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 1191
Size: 238 953
BsaBI GATnn'nnATC
Cuts at: 0 1071 1191
Size: 1071 120
BsaHI Gr'CG_yC
Cuts at: 0 1110 1191
Size: 1110 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1008 1049 1191
Size: 1008 41 142
Fragments arranged by size:
1008 142 41
BseYI C'CCAG_C
Cuts at: 0 249 1191
Size: 249 942
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1191
Size: 1070 121
BsiHKAI G_wGCw'C
Cuts at: 0 1186 1191
Size: 1186 5
BsmI GAATG_Cn'
Cuts at: 0 750 1191
Size: 750 441
Bsp1286I G_dGCh'C
Cuts at: 0 909 1186 1191
Size: 909 277 5
Fragments arranged by size:
909 277 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 1191
Size: 642 549
BsrDI GCAATG_nn'
Cuts at: 0 177 491 1040 1191
Size: 177 314 549 151
Fragments arranged by size:
549 314 177 151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 490 745 1191
Size: 490 255 446
Fragments arranged by size:
490 446 255
BstEII G'GTnAC_C
Cuts at: 0 1051 1191
Size: 1051 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1047 1191
Size: 1047 144
BstYI r'GATC_y
Cuts at: 0 1002 1066 1191
Size: 1002 64 125
Fragments arranged by size:
1002 125 64
BtgI C'CryG_G
Cuts at: 0 479 1191
Size: 479 712
BtsI GCAGTG_nn'
Cuts at: 0 80 1191
Size: 80 1111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 818 853 1191
Size: 818 35 338
Fragments arranged by size:
818 338 35
EaeI y'GGCC_r
Cuts at: 0 1155 1191
Size: 1155 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 353 692 791 931 1191
Size: 173 180 339 99 140 260
Fragments arranged by size:
339 260 180 173 140 99
EcoRV GAT'ATC
Cuts at: 0 423 1191
Size: 423 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 1191
Size: 135 1056
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 407 439 764 796 987 1019
Size: 237 32 138 32 325 32 191 32
Cuts at: 1019 1191
Size: 172
Fragments arranged by size:
325 237 191 172 138 32 32 32
32
HincII GTy'rAC
Cuts at: 0 153 1191
Size: 153 1038
HindIII A'AGCT_T
Cuts at: 0 409 962 1191
Size: 409 553 229
Fragments arranged by size:
553 409 229
MfeI C'AATT_G
Cuts at: 0 614 1191
Size: 614 577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1191
Size: 1113 78
MscI TGG'CCA
Cuts at: 0 1157 1191
Size: 1157 34
MslI CAynn'nnrTG
Cuts at: 0 484 1191
Size: 484 707
NcoI C'CATG_G
Cuts at: 0 479 1191
Size: 479 712
NsiI A_TGCA'T
Cuts at: 0 750 1191
Size: 750 441
NspI r_CATG'y
Cuts at: 0 217 469 667 1191
Size: 217 252 198 524
Fragments arranged by size:
524 252 217 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 1191
Size: 981 210
PstI C_TGCA'G
Cuts at: 0 182 495 554 741 1191
Size: 182 313 59 187 450
Fragments arranged by size:
450 313 187 182 59
ScaI AGT'ACT
Cuts at: 0 979 1191
Size: 979 212
SexAI A'CCwGG_T
Cuts at: 0 1091 1191
Size: 1091 100
SfcI C'TryA_G
Cuts at: 0 178 491 550 737 1191
Size: 178 313 59 187 454
Fragments arranged by size:
454 313 187 178 59
SmlI C'TyrA_G
Cuts at: 0 931 1191
Size: 931 260
SphI G_CATG'C
Cuts at: 0 667 1191
Size: 667 524
StuI AGG'CCT
Cuts at: 0 65 1191
Size: 65 1126
StyI C'CwwG_G
Cuts at: 0 110 479 1158 1191
Size: 110 369 679 33
Fragments arranged by size:
679 369 110 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1070 1191
Size: 1070 121
TatI w'GTAC_w
Cuts at: 0 977 1191
Size: 977 214
Tth111I GACn'n_nGTC
Cuts at: 0 926 1191
Size: 926 265
XmnI GAAnn'nnTTC
Cuts at: 0 76 1191
Size: 76 1115
Enzymes that do cut:
AccI AcuI AflII AlfI ApoI AseI BanI BbsI
BfrBI BlpI Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI
EaeI Eco57MI EcoRV FalI Hin4I HincII HindIII MfeI
MmeI MscI MslI NcoI NsiI NspI PflMI PstI
ScaI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BmgBI BmtI Bpu10I BpuEI BsaAI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI
ZraI