Restriction map for
pF1KE1044
(Linear) (Six-base) MAPSORT of: KIEE1044.seq Check: 7589 from: 1 to: 1746
KIEE1044.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 403 416 1583 1746
Size: 325 78 13 1167 163
Fragments arranged by size:
1167 325 163 78 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1049 1746
Size: 1049 697
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 1746
Size: 1129 32 585
Fragments arranged by size:
1129 585 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1183 1746
Size: 1183 563
ApaI G_GGCC'C
Cuts at: 0 1334 1746
Size: 1334 412
ApoI r'AATT_y
Cuts at: 0 7 56 880 1111 1641 1746
Size: 7 49 824 231 530 105
Fragments arranged by size:
824 530 231 105 49 7
AvaI C'yCGr_G
Cuts at: 0 1646 1746
Size: 1646 100
BanI G'GyrC_C
Cuts at: 0 328 1424 1746
Size: 328 1096 322
Fragments arranged by size:
1096 328 322
BanII G_rGCy'C
Cuts at: 0 896 1334 1746
Size: 896 438 412
Fragments arranged by size:
896 438 412
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 1280 1746
Size: 391 889 466
Fragments arranged by size:
889 466 391
BfrBI ATG'CAT
Cuts at: 0 454 1746
Size: 454 1292
BglI GCCn_nnn'nGGC
Cuts at: 0 549 1260 1746
Size: 549 711 486
Fragments arranged by size:
711 549 486
BglII A'GATC_T
Cuts at: 0 378 1746
Size: 378 1368
BlpI GC'TnA_GC
Cuts at: 0 1456 1746
Size: 1456 290
Bme1580I G_kGCm'C
Cuts at: 0 250 333 1334 1529 1580 1746
Size: 250 83 1001 195 51 166
Fragments arranged by size:
1001 250 195 166 83 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 807 1746
Size: 747 60 939
Fragments arranged by size:
939 747 60
Bpu10I CC'TnA_GC
Cuts at: 0 1066 1746
Size: 1066 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1746
Size: 1217 529
BsaI GGTCTCn'nnnn_
Cuts at: 0 836 1231 1274 1746
Size: 836 395 43 472
Fragments arranged by size:
836 472 395 43
BsaAI yAC'GTr
Cuts at: 0 946 1746
Size: 946 800
BsaBI GATnn'nnATC
Cuts at: 0 1404 1746
Size: 1404 342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 1129 1159 1617 1647 1746
Size: 229 30 870 30 458 30 99
Fragments arranged by size:
870 458 229 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 493 904 1102 1150 1538 1746
Size: 250 243 411 198 48 388 208
Fragments arranged by size:
411 388 250 243 208 198 48
BseYI C'CCAG_C
Cuts at: 0 1176 1226 1351 1432 1717 1746
Size: 1176 50 125 81 285 29
Fragments arranged by size:
1176 285 125 81 50 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1746
Size: 872 874
BsiHKAI G_wGCw'C
Cuts at: 0 896 1746
Size: 896 850
Bsp1286I G_dGCh'C
Cuts at: 0 250 333 896 1334 1529 1580 1746
Size: 250 83 563 438 195 51 166
Fragments arranged by size:
563 438 250 195 166 83 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 196 1746
Size: 196 1550
BsrDI GCAATG_nn'
Cuts at: 0 369 1746
Size: 369 1377
BstEII G'GTnAC_C
Cuts at: 0 183 1294 1650 1746
Size: 183 1111 356 96
Fragments arranged by size:
1111 356 183 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 817 990 1746
Size: 817 173 756
Fragments arranged by size:
817 756 173
BstYI r'GATC_y
Cuts at: 0 86 378 1746
Size: 86 292 1368
Fragments arranged by size:
1368 292 86
BtsI GCAGTG_nn'
Cuts at: 0 1181 1746
Size: 1181 565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 678 713 1746
Size: 678 35 1033
Fragments arranged by size:
1033 678 35
DraIII CAC_nnn'GTG
Cuts at: 0 1083 1746
Size: 1083 663
EarI CTCTTCn'nnn_
Cuts at: 0 208 698 981 1127 1724 1730 1746
Size: 208 490 283 146 597 6 16
Fragments arranged by size:
597 490 283 208 146 16 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1503 1746
Size: 1503 243
EcoICRI GAG'CTC
Cuts at: 0 894 1746
Size: 894 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 403 416 747 807 1583 1746
Size: 325 78 13 331 60 776 163
Fragments arranged by size:
776 331 325 163 78 60 13
EcoO109I rG'GnC_Cy
Cuts at: 0 551 1411 1746
Size: 551 860 335
Fragments arranged by size:
860 551 335
EcoRI G'AATT_C
Cuts at: 0 1111 1641 1746
Size: 1111 530 105
Fragments arranged by size:
1111 530 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 906 938 1530 1562 1746
Size: 78 32 796 32 592 32 184
Fragments arranged by size:
796 592 184 78 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1746
Size: 188 1558
MslI CAynn'nnrTG
Cuts at: 0 109 1746
Size: 109 1637
MspA1I CmG'CkG
Cuts at: 0 856 1128 1663 1746
Size: 856 272 535 83
Fragments arranged by size:
856 535 272 83
NdeI CA'TA_TG
Cuts at: 0 456 1746
Size: 456 1290
NsiI A_TGCA'T
Cuts at: 0 456 1746
Size: 456 1290
PasI CC'CwG_GG
Cuts at: 0 1522 1591 1746
Size: 1522 69 155
Fragments arranged by size:
1522 155 69
PmlI CAC'GTG
Cuts at: 0 946 1746
Size: 946 800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1129 1161 1746
Size: 1129 32 585
Fragments arranged by size:
1129 585 32
PspOMI G'GGCC_C
Cuts at: 0 1330 1746
Size: 1330 416
PstI C_TGCA'G
Cuts at: 0 171 1662 1746
Size: 171 1491 84
Fragments arranged by size:
1491 171 84
PvuII CAG'CTG
Cuts at: 0 856 1128 1663 1746
Size: 856 272 535 83
Fragments arranged by size:
856 535 272 83
SacI G_AGCT'C
Cuts at: 0 896 1746
Size: 896 850
SapI GCTCTTCn'nnn_
Cuts at: 0 981 1746
Size: 981 765
SfcI C'TryA_G
Cuts at: 0 167 1658 1746
Size: 167 1491 88
Fragments arranged by size:
1491 167 88
SmaI CCC'GGG
Cuts at: 0 1648 1746
Size: 1648 98
SmlI C'TyrA_G
Cuts at: 0 1232 1746
Size: 1232 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 546 1412 1746
Size: 546 866 334
Fragments arranged by size:
866 546 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1617 1649 1746
Size: 229 32 1356 32 97
Fragments arranged by size:
1356 229 97 32 32
XbaI T'CTAG_A
Cuts at: 0 12 1746
Size: 12 1734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1746
Size: 147 1599
XmaI C'CCGG_G
Cuts at: 0 1646 1746
Size: 1646 100
XmnI GAAnn'nnTTC
Cuts at: 0 134 1746
Size: 134 1612
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BfrBI BglI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BstEII BstXI BstYI
BtsI CspCI DraIII EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI Hin4I MmeI MslI MspA1I NdeI NsiI PasI
PmlI PpiI PspOMI PstI PvuII SacI SapI SfcI
SmaI SmlI TaqII TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BmgBI
BmrI BmtI BplI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DrdI EaeI EagI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PfoI PmeI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XhoI ZraI