Restriction map for
pF1KE1040
(Linear) (Six-base) MAPSORT of: KIEE1040.seq Check: 9697 from: 1 to: 1344
KIEE1040.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 455 1344
Size: 455 889
AccI GT'mk_AC
Cuts at: 0 340 1344
Size: 340 1004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 427 844 985 1238 1344
Size: 91 336 417 141 253 106
Fragments arranged by size:
417 336 253 141 106 91
AflIII A'CryG_T
Cuts at: 0 48 1127 1344
Size: 48 1079 217
Fragments arranged by size:
1079 217 48
AhdI GACnn_n'nnGTC
Cuts at: 0 1128 1344
Size: 1128 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 854 1270 1344
Size: 854 416 74
Fragments arranged by size:
854 416 74
AlwNI CAG_nnn'CTG
Cuts at: 0 336 480 1344
Size: 336 144 864
Fragments arranged by size:
864 336 144
AvaI C'yCGr_G
Cuts at: 0 1003 1344
Size: 1003 341
BamHI G'GATC_C
Cuts at: 0 284 497 1344
Size: 284 213 847
Fragments arranged by size:
847 284 213
BanII G_rGCy'C
Cuts at: 0 7 1344
Size: 7 1337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 67 595 629 1344
Size: 33 34 528 34 715
Fragments arranged by size:
715 528 34 34 33
BglII A'GATC_T
Cuts at: 0 90 1344
Size: 90 1254
BlpI GC'TnA_GC
Cuts at: 0 16 40 667 1344
Size: 16 24 627 677
Fragments arranged by size:
677 627 24 16
BmgBI CAC'GTC
Cuts at: 0 51 1344
Size: 51 1293
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 1330 1344
Size: 384 946 14
Fragments arranged by size:
946 384 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 523 1344
Size: 502 21 821
Fragments arranged by size:
821 502 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1326 1344
Size: 1326 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 267 1164 1344
Size: 267 897 180
Fragments arranged by size:
897 267 180
BsaAI yAC'GTr
Cuts at: 0 1128 1344
Size: 1128 216
BsaHI Gr'CG_yC
Cuts at: 0 868 1344
Size: 868 476
BsaWI w'CCGG_w
Cuts at: 0 119 494 1344
Size: 119 375 850
Fragments arranged by size:
850 375 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 1344
Size: 1222 122
BseYI C'CCAG_C
Cuts at: 0 1066 1344
Size: 1066 278
BsiEI CG_ry'CG
Cuts at: 0 807 1344
Size: 807 537
BsiHKAI G_wGCw'C
Cuts at: 0 7 488 1344
Size: 7 481 856
Fragments arranged by size:
856 481 7
BsiWI C'GTAC_G
Cuts at: 0 914 1344
Size: 914 430
Bsp1286I G_dGCh'C
Cuts at: 0 7 488 1344
Size: 7 481 856
Fragments arranged by size:
856 481 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 455 1344
Size: 110 345 889
Fragments arranged by size:
889 345 110
BsrBI CCG'CTC
Cuts at: 0 20 1344
Size: 20 1324
BsrDI GCAATG_nn'
Cuts at: 0 671 1344
Size: 671 673
BsrFI r'CCGG_y
Cuts at: 0 864 1344
Size: 864 480
BsrGI T'GTAC_A
Cuts at: 0 1094 1344
Size: 1094 250
BssSI C'ACGA_G
Cuts at: 0 210 1344
Size: 210 1134
BstYI r'GATC_y
Cuts at: 0 90 284 497 1289 1344
Size: 90 194 213 792 55
Fragments arranged by size:
792 213 194 90 55
BtgI C'CryG_G
Cuts at: 0 1019 1075 1344
Size: 1019 56 269
Fragments arranged by size:
1019 269 56
DraIII CAC_nnn'GTG
Cuts at: 0 54 1324 1344
Size: 54 1270 20
Fragments arranged by size:
1270 54 20
DrdI GACnn_nn'nnGTC
Cuts at: 0 1036 1156 1344
Size: 1036 120 188
Fragments arranged by size:
1036 188 120
EarI CTCTTCn'nnn_
Cuts at: 0 730 1053 1344
Size: 730 323 291
Fragments arranged by size:
730 323 291
EcoICRI GAG'CTC
Cuts at: 0 5 1344
Size: 5 1339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 427 502 523 844 985 1238 1344
Size: 91 336 75 21 321 141 253 106
Fragments arranged by size:
336 321 253 141 106 91 75 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1180 1344
Size: 1180 164
EcoO109I rG'GnC_Cy
Cuts at: 0 1008 1314 1344
Size: 1008 306 30
Fragments arranged by size:
1008 306 30
HaeII r_GCGC'y
Cuts at: 0 82 1344
Size: 82 1262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 739 771 1114 1146 1344
Size: 383 32 324 32 343 32 198
Fragments arranged by size:
383 343 324 198 32 32 32
HincII GTy'rAC
Cuts at: 0 396 1344
Size: 396 948
HindIII A'AGCT_T
Cuts at: 0 409 1344
Size: 409 935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 782 1344
Size: 488 294 562
Fragments arranged by size:
562 488 294
MslI CAynn'nnrTG
Cuts at: 0 232 1344
Size: 232 1112
MspA1I CmG'CkG
Cuts at: 0 111 477 693 987 1344
Size: 111 366 216 294 357
Fragments arranged by size:
366 357 294 216 111
NaeI GCC'GGC
Cuts at: 0 866 1344
Size: 866 478
NgoMIV G'CCGG_C
Cuts at: 0 864 1344
Size: 864 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 480 1344
Size: 480 864
PfoI T'CCnGG_A
Cuts at: 0 500 542 698 707 1344
Size: 500 42 156 9 637
Fragments arranged by size:
637 500 156 42 9
PpuMI rG'GwC_Cy
Cuts at: 0 1008 1314 1344
Size: 1008 306 30
Fragments arranged by size:
1008 306 30
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1344
Size: 1171 32 141
Fragments arranged by size:
1171 141 32
PstI C_TGCA'G
Cuts at: 0 1168 1344
Size: 1168 176
PvuI CG_AT'CG
Cuts at: 0 807 1344
Size: 807 537
PvuII CAG'CTG
Cuts at: 0 111 1344
Size: 111 1233
SacI G_AGCT'C
Cuts at: 0 7 1344
Size: 7 1337
SexAI A'CCwGG_T
Cuts at: 0 1181 1344
Size: 1181 163
SfcI C'TryA_G
Cuts at: 0 897 1164 1242 1344
Size: 897 267 78 102
Fragments arranged by size:
897 267 102 78
SgrAI Cr'CCGG_yG
Cuts at: 0 864 1344
Size: 864 480
SmlI C'TyrA_G
Cuts at: 0 1305 1344
Size: 1305 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 1312 1344
Size: 817 495 32
Fragments arranged by size:
817 495 32
TatI w'GTAC_w
Cuts at: 0 1094 1344
Size: 1094 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 1344
Size: 372 32 940
Fragments arranged by size:
940 372 32
XbaI T'CTAG_A
Cuts at: 0 87 1344
Size: 87 1257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1294 1344
Size: 1294 50
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI AvaI
BamHI BanII BcgI BglII BlpI BmgBI BmrI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstYI BtgI DraIII DrdI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NaeI NgoMIV PflMI PfoI PpuMI PsrI PstI
PvuI PvuII SacI SexAI SfcI SgrAI SmlI TaqII
TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI
Bme1580I BmtI BplI Bpu10I BsaBI BsaXI BsgI BsmI
BsmBI BspEI BspHI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EaeI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI Tth111I XhoI XmaI XmnI ZraI