Restriction map for
pF1KE1018
(Linear) (Six-base) MAPSORT of: KIEE1018.seq Check: 3058 from: 1 to: 1917
KIEE1018.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 666 1917
Size: 666 1251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 879 1551 1917
Size: 711 168 672 366
Fragments arranged by size:
711 672 366 168
AlwNI CAG_nnn'CTG
Cuts at: 0 13 129 1917
Size: 13 116 1788
Fragments arranged by size:
1788 116 13
ApoI r'AATT_y
Cuts at: 0 1601 1917
Size: 1601 316
BfrBI ATG'CAT
Cuts at: 0 1711 1917
Size: 1711 206
BmgBI CAC'GTC
Cuts at: 0 162 1917
Size: 162 1755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1043 1799 1917
Size: 190 853 756 118
Fragments arranged by size:
853 756 190 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1917
Size: 214 1703
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 855 1917
Size: 166 689 1062
Fragments arranged by size:
1062 689 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 1917
Size: 109 30 1778
Fragments arranged by size:
1778 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1917
Size: 130 1787
BsiHKAI G_wGCw'C
Cuts at: 0 402 1917
Size: 402 1515
BsmI GAATG_Cn'
Cuts at: 0 253 1045 1917
Size: 253 792 872
Fragments arranged by size:
872 792 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 152 1917
Size: 152 1765
Bsp1286I G_dGCh'C
Cuts at: 0 402 1917
Size: 402 1515
BspHI T'CATG_A
Cuts at: 0 411 1917
Size: 411 1506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 1917
Size: 195 1722
BsrDI GCAATG_nn'
Cuts at: 0 1470 1917
Size: 1470 447
BsrGI T'GTAC_A
Cuts at: 0 152 750 1338 1758 1798 1842 1917
Size: 152 598 588 420 40 44 75
Fragments arranged by size:
598 588 420 152 75 44 40
BssSI C'ACGA_G
Cuts at: 0 401 1917
Size: 401 1516
BstEII G'GTnAC_C
Cuts at: 0 207 1917
Size: 207 1710
BtgI C'CryG_G
Cuts at: 0 1875 1917
Size: 1875 42
DraIII CAC_nnn'GTG
Cuts at: 0 1721 1917
Size: 1721 196
EarI CTCTTCn'nnn_
Cuts at: 0 1875 1917
Size: 1875 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 711 879 1043 1551 1799 1917
Size: 190 521 168 164 508 248 118
Fragments arranged by size:
521 508 248 190 168 164 118
EcoO109I rG'GnC_Cy
Cuts at: 0 146 1917
Size: 146 1771
EcoRI G'AATT_C
Cuts at: 0 1601 1917
Size: 1601 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 309 341 1917
Size: 309 32 1576
Fragments arranged by size:
1576 309 32
HincII GTy'rAC
Cuts at: 0 908 1160 1244 1748 1917
Size: 908 252 84 504 169
Fragments arranged by size:
908 504 252 169 84
HindIII A'AGCT_T
Cuts at: 0 36 806 1072 1646 1917
Size: 36 770 266 574 271
Fragments arranged by size:
770 574 271 266 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 342 1016 1621 1917
Size: 215 127 674 605 296
Fragments arranged by size:
674 605 296 215 127
MslI CAynn'nnrTG
Cuts at: 0 329 1917
Size: 329 1588
NcoI C'CATG_G
Cuts at: 0 1875 1917
Size: 1875 42
NsiI A_TGCA'T
Cuts at: 0 1713 1917
Size: 1713 204
NspI r_CATG'y
Cuts at: 0 716 1715 1917
Size: 716 999 202
Fragments arranged by size:
999 716 202
PfoI T'CCnGG_A
Cuts at: 0 600 1917
Size: 600 1317
PpuMI rG'GwC_Cy
Cuts at: 0 146 1917
Size: 146 1771
PstI C_TGCA'G
Cuts at: 0 537 1917
Size: 537 1380
SfcI C'TryA_G
Cuts at: 0 177 533 1917
Size: 177 356 1384
Fragments arranged by size:
1384 356 177
SmlI C'TyrA_G
Cuts at: 0 229 1917
Size: 229 1688
SphI G_CATG'C
Cuts at: 0 1715 1917
Size: 1715 202
StuI AGG'CCT
Cuts at: 0 659 1917
Size: 659 1258
StyI C'CwwG_G
Cuts at: 0 1332 1875 1917
Size: 1332 543 42
Fragments arranged by size:
1332 543 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 1917
Size: 124 1793
TatI w'GTAC_w
Cuts at: 0 152 439 706 750 1210 1338 1758 1798
Size: 152 287 267 44 460 128 420 40
Cuts at: 1798 1842 1917
Size: 44 75
Fragments arranged by size:
460 420 287 267 152 128 75 44
44 40
XmnI GAAnn'nnTTC
Cuts at: 0 724 1144 1228 1353 1396 1917
Size: 724 420 84 125 43 521
Fragments arranged by size:
724 521 420 125 84 43
Enzymes that do cut:
AccI AcuI AlwNI ApoI BfrBI BmgBI BpmI BpuEI
BsaI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstEII BtgI DraIII EarI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI
NcoI NsiI NspI PfoI PpuMI PstI SfcI SmlI
SphI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI