Restriction map for
pF1KE1016
(Linear) (Six-base) MAPSORT of: KIEE1016.seq Check: 1298 from: 1 to: 1320
KIEE1016.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 0 1045 1320
Size: 1045 275
AclI AA'CG_TT
Cuts at: 0 716 1320
Size: 716 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 730 1150 1320
Size: 51 679 420 170
Fragments arranged by size:
679 420 170 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 1320
Size: 379 941
AlwNI CAG_nnn'CTG
Cuts at: 0 74 431 548 676 866 918 1168 1203
Size: 74 357 117 128 190 52 250 35
Cuts at: 1203 1320
Size: 117
Fragments arranged by size:
357 250 190 128 117 117 74 52
35
ApaLI G'TGCA_C
Cuts at: 0 364 1320
Size: 364 956
ApoI r'AATT_y
Cuts at: 0 702 1320
Size: 702 618
AvaI C'yCGr_G
Cuts at: 0 85 1320
Size: 85 1235
BamHI G'GATC_C
Cuts at: 0 690 1320
Size: 690 630
BanI G'GyrC_C
Cuts at: 0 348 518 904 959 1320
Size: 348 170 386 55 361
Fragments arranged by size:
386 361 348 170 55
BanII G_rGCy'C
Cuts at: 0 622 1320
Size: 622 698
BbeI G_GCGC'C
Cuts at: 0 352 908 963 1320
Size: 352 556 55 357
Fragments arranged by size:
556 357 352 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 1320
Size: 1016 304
BglI GCCn_nnn'nGGC
Cuts at: 0 752 1320
Size: 752 568
BlpI GC'TnA_GC
Cuts at: 0 142 1320
Size: 142 1178
Bme1580I G_kGCm'C
Cuts at: 0 368 488 521 1320
Size: 368 120 33 799
Fragments arranged by size:
799 368 120 33
BmgBI CAC'GTC
Cuts at: 0 625 1320
Size: 625 695
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 552 1209 1320
Size: 552 657 111
Fragments arranged by size:
657 552 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 800 1042 1320
Size: 636 164 242 278
Fragments arranged by size:
636 278 242 164
Bpu10I CC'TnA_GC
Cuts at: 0 341 742 1320
Size: 341 401 578
Fragments arranged by size:
578 401 341
BsaAI yAC'GTr
Cuts at: 0 267 1320
Size: 267 1053
BsaHI Gr'CG_yC
Cuts at: 0 4 349 905 960 1320
Size: 4 345 556 55 360
Fragments arranged by size:
556 360 345 55 4
BsaWI w'CCGG_w
Cuts at: 0 554 1320
Size: 554 766
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 609 639 1320
Size: 609 30 681
Fragments arranged by size:
681 609 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 620 850 1013 1320
Size: 620 230 163 307
Fragments arranged by size:
620 307 230 163
BseYI C'CCAG_C
Cuts at: 0 505 877 1320
Size: 505 372 443
Fragments arranged by size:
505 443 372
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 656 1320
Size: 444 212 664
Fragments arranged by size:
664 444 212
BsiHKAI G_wGCw'C
Cuts at: 0 342 368 782 1320
Size: 342 26 414 538
Fragments arranged by size:
538 414 342 26
BsmI GAATG_Cn'
Cuts at: 0 499 1320
Size: 499 821
BsmBI CGTCTCn'nnnn_
Cuts at: 0 816 1320
Size: 816 504
Bsp1286I G_dGCh'C
Cuts at: 0 342 368 488 521 622 782 1320
Size: 342 26 120 33 101 160 538
Fragments arranged by size:
538 342 160 120 101 33 26
BspEI T'CCGG_A
Cuts at: 0 554 1320
Size: 554 766
BsrBI CCG'CTC
Cuts at: 0 135 1276 1320
Size: 135 1141 44
Fragments arranged by size:
1141 135 44
BsrDI GCAATG_nn'
Cuts at: 0 399 1320
Size: 399 921
BsrFI r'CCGG_y
Cuts at: 0 314 1320
Size: 314 1006
BssHII G'CGCG_C
Cuts at: 0 49 1320
Size: 49 1271
BssSI C'ACGA_G
Cuts at: 0 632 1320
Size: 632 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 431 866 1320
Size: 431 435 454
Fragments arranged by size:
454 435 431
BstXI CCAn_nnnn'nTGG
Cuts at: 0 614 1320
Size: 614 706
BstYI r'GATC_y
Cuts at: 0 690 1320
Size: 690 630
Bsu36I CC'TnA_GG
Cuts at: 0 92 965 1320
Size: 92 873 355
Fragments arranged by size:
873 355 92
BtgI C'CryG_G
Cuts at: 0 659 1174 1208 1320
Size: 659 515 34 112
Fragments arranged by size:
659 515 112 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 218 398 1320
Size: 218 180 922
Fragments arranged by size:
922 218 180
ClaI AT'CG_AT
Cuts at: 0 770 1320
Size: 770 550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 870 905 1016 1051 1320
Size: 870 35 111 35 269
Fragments arranged by size:
870 269 111 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 337 1320
Size: 337 983
DrdI GACnn_nn'nnGTC
Cuts at: 0 1020 1320
Size: 1020 300
EaeI y'GGCC_r
Cuts at: 0 152 254 583 1320
Size: 152 102 329 737
Fragments arranged by size:
737 329 152 102
EarI CTCTTCn'nnn_
Cuts at: 0 835 1320
Size: 835 485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 937 1320
Size: 112 825 383
Fragments arranged by size:
825 383 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 636 730 800 1042 1150 1320
Size: 51 585 94 70 242 108 170
Fragments arranged by size:
585 242 170 108 94 70 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 404 526 635 1320
Size: 404 122 109 685
Fragments arranged by size:
685 404 122 109
EcoO109I rG'GnC_Cy
Cuts at: 0 124 1117 1320
Size: 124 993 203
Fragments arranged by size:
993 203 124
EcoRV GAT'ATC
Cuts at: 0 768 1320
Size: 768 552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 960 1320
Size: 960 360
HaeII r_GCGC'y
Cuts at: 0 352 908 963 1320
Size: 352 556 55 357
Fragments arranged by size:
556 357 352 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 609 641 1320
Size: 609 32 679
Fragments arranged by size:
679 609 32
KasI G'GCGC_C
Cuts at: 0 348 904 959 1320
Size: 348 556 55 361
Fragments arranged by size:
556 361 348 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1320
Size: 962 358
MscI TGG'CCA
Cuts at: 0 256 585 1320
Size: 256 329 735
Fragments arranged by size:
735 329 256
MslI CAynn'nnrTG
Cuts at: 0 371 1320
Size: 371 949
MspA1I CmG'CkG
Cuts at: 0 548 736 863 1176 1320
Size: 548 188 127 313 144
Fragments arranged by size:
548 313 188 144 127
NaeI GCC'GGC
Cuts at: 0 316 1320
Size: 316 1004
NarI GG'CG_CC
Cuts at: 0 349 905 960 1320
Size: 349 556 55 360
Fragments arranged by size:
556 360 349 55
NcoI C'CATG_G
Cuts at: 0 1208 1320
Size: 1208 112
NgoMIV G'CCGG_C
Cuts at: 0 314 1320
Size: 314 1006
PfoI T'CCnGG_A
Cuts at: 0 120 1320
Size: 120 1200
PpuMI rG'GwC_Cy
Cuts at: 0 124 1117 1320
Size: 124 993 203
Fragments arranged by size:
993 203 124
PshAI GACnn'nnGTC
Cuts at: 0 808 1320
Size: 808 512
PstI C_TGCA'G
Cuts at: 0 82 1253 1320
Size: 82 1171 67
Fragments arranged by size:
1171 82 67
PvuII CAG'CTG
Cuts at: 0 736 863 1320
Size: 736 127 457
Fragments arranged by size:
736 457 127
SacII CC_GC'GG
Cuts at: 0 1177 1320
Size: 1177 143
SanDI GG'GwC_CC
Cuts at: 0 124 1117 1320
Size: 124 993 203
Fragments arranged by size:
993 203 124
SapI GCTCTTCn'nnn_
Cuts at: 0 835 1320
Size: 835 485
SfcI C'TryA_G
Cuts at: 0 78 1046 1066 1249 1320
Size: 78 968 20 183 71
Fragments arranged by size:
968 183 78 71 20
SfoI GGC'GCC
Cuts at: 0 350 906 961 1320
Size: 350 556 55 359
Fragments arranged by size:
556 359 350 55
StuI AGG'CCT
Cuts at: 0 22 1320
Size: 22 1298
StyI C'CwwG_G
Cuts at: 0 944 1208 1320
Size: 944 264 112
Fragments arranged by size:
944 264 112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 611 915 1320
Size: 611 304 405
Fragments arranged by size:
611 405 304
Enzymes that do cut:
AccI AclI AcuI AlfI AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PfoI PpuMI PshAI PstI PvuII SacII
SanDI SapI SfcI SfoI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI
BpuEI BsaI BsaBI BsiEI BsiWI BspHI BspMI BsrGI
BstBI BstEII BstZ17I BtsI DraI EagI EcoICRI EcoRI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI