Restriction map for
pF1KE1015
(Linear) (Six-base) MAPSORT of: KIEE1015.seq Check: 6228 from: 1 to: 990
KIEE1015.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 990
Size: 484 506
AflIII A'CryG_T
Cuts at: 0 45 990
Size: 45 945
AgeI A'CCGG_T
Cuts at: 0 560 990
Size: 560 430
ApaI G_GGCC'C
Cuts at: 0 924 990
Size: 924 66
ApaLI G'TGCA_C
Cuts at: 0 231 990
Size: 231 759
BamHI G'GATC_C
Cuts at: 0 35 668 935 990
Size: 35 633 267 55
Fragments arranged by size:
633 267 55 35
BanI G'GyrC_C
Cuts at: 0 139 894 990
Size: 139 755 96
Fragments arranged by size:
755 139 96
BanII G_rGCy'C
Cuts at: 0 74 165 185 478 924 990
Size: 74 91 20 293 446 66
Fragments arranged by size:
446 293 91 74 66 20
BbeI G_GCGC'C
Cuts at: 0 898 990
Size: 898 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 632 990
Size: 598 34 358
Fragments arranged by size:
598 358 34
BglI GCCn_nnn'nGGC
Cuts at: 0 826 847 928 990
Size: 826 21 81 62
Fragments arranged by size:
826 81 62 21
Bme1580I G_kGCm'C
Cuts at: 0 142 235 924 990
Size: 142 93 689 66
Fragments arranged by size:
689 142 93 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 990
Size: 252 738
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 990
Size: 88 902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 895 946 990
Size: 364 531 51 44
Fragments arranged by size:
531 364 51 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 990
Size: 610 380
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 990
Size: 214 776
BsaAI yAC'GTr
Cuts at: 0 485 990
Size: 485 505
BsaHI Gr'CG_yC
Cuts at: 0 416 895 990
Size: 416 479 95
Fragments arranged by size:
479 416 95
BsaWI w'CCGG_w
Cuts at: 0 113 560 990
Size: 113 447 430
Fragments arranged by size:
447 430 113
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 748 990
Size: 80 668 242
Fragments arranged by size:
668 242 80
BseYI C'CCAG_C
Cuts at: 0 434 885 990
Size: 434 451 105
Fragments arranged by size:
451 434 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 990
Size: 106 884
BsiEI CG_ry'CG
Cuts at: 0 830 990
Size: 830 160
BsiHKAI G_wGCw'C
Cuts at: 0 74 185 235 990
Size: 74 111 50 755
Fragments arranged by size:
755 111 74 50
BsmI GAATG_Cn'
Cuts at: 0 843 990
Size: 843 147
Bsp1286I G_dGCh'C
Cuts at: 0 74 142 165 185 235 478 924 990
Size: 74 68 23 20 50 243 446 66
Fragments arranged by size:
446 243 74 68 66 50 23 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 641 990
Size: 641 349
BsrDI GCAATG_nn'
Cuts at: 0 614 990
Size: 614 376
BsrFI r'CCGG_y
Cuts at: 0 560 990
Size: 560 430
BssSI C'ACGA_G
Cuts at: 0 164 990
Size: 164 826
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 990
Size: 873 117
BstYI r'GATC_y
Cuts at: 0 35 668 935 977 990
Size: 35 633 267 42 13
Fragments arranged by size:
633 267 42 35 13
BtgI C'CryG_G
Cuts at: 0 176 499 990
Size: 176 323 491
Fragments arranged by size:
491 323 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 306 990
Size: 306 684
BtsI GCAGTG_nn'
Cuts at: 0 357 539 756 990
Size: 357 182 217 234
Fragments arranged by size:
357 234 217 182
DrdI GACnn_nn'nnGTC
Cuts at: 0 790 990
Size: 790 200
EaeI y'GGCC_r
Cuts at: 0 302 827 990
Size: 302 525 163
Fragments arranged by size:
525 302 163
EagI C'GGCC_G
Cuts at: 0 827 990
Size: 827 163
EarI CTCTTCn'nnn_
Cuts at: 0 512 768 947 990
Size: 512 256 179 43
Fragments arranged by size:
512 256 179 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 990
Size: 19 971
EcoICRI GAG'CTC
Cuts at: 0 72 183 990
Size: 72 111 807
Fragments arranged by size:
807 111 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 484 895 946 990
Size: 364 120 411 51 44
Fragments arranged by size:
411 364 120 51 44
EcoO109I rG'GnC_Cy
Cuts at: 0 336 495 656 921 990
Size: 336 159 161 265 69
Fragments arranged by size:
336 265 161 159 69
EcoRV GAT'ATC
Cuts at: 0 9 990
Size: 9 981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 990
Size: 819 171
HaeII r_GCGC'y
Cuts at: 0 898 990
Size: 898 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 745 777 990
Size: 745 32 213
Fragments arranged by size:
745 213 32
KasI G'GCGC_C
Cuts at: 0 894 990
Size: 894 96
MscI TGG'CCA
Cuts at: 0 304 990
Size: 304 686
MslI CAynn'nnrTG
Cuts at: 0 325 457 990
Size: 325 132 533
Fragments arranged by size:
533 325 132
MspA1I CmG'CkG
Cuts at: 0 297 354 675 696 990
Size: 297 57 321 21 294
Fragments arranged by size:
321 297 294 57 21
NarI GG'CG_CC
Cuts at: 0 895 990
Size: 895 95
NcoI C'CATG_G
Cuts at: 0 176 499 990
Size: 176 323 491
Fragments arranged by size:
491 323 176
NspI r_CATG'y
Cuts at: 0 283 583 990
Size: 283 300 407
Fragments arranged by size:
407 300 283
PasI CC'CwG_GG
Cuts at: 0 396 990
Size: 396 594
PfoI T'CCnGG_A
Cuts at: 0 23 74 990
Size: 23 51 916
Fragments arranged by size:
916 51 23
PpuMI rG'GwC_Cy
Cuts at: 0 336 495 656 990
Size: 336 159 161 334
Fragments arranged by size:
336 334 161 159
PspOMI G'GGCC_C
Cuts at: 0 920 990
Size: 920 70
PstI C_TGCA'G
Cuts at: 0 425 638 990
Size: 425 213 352
Fragments arranged by size:
425 352 213
PvuII CAG'CTG
Cuts at: 0 675 990
Size: 675 315
SacI G_AGCT'C
Cuts at: 0 74 185 990
Size: 74 111 805
Fragments arranged by size:
805 111 74
SanDI GG'GwC_CC
Cuts at: 0 656 990
Size: 656 334
SapI GCTCTTCn'nnn_
Cuts at: 0 768 990
Size: 768 222
ScaI AGT'ACT
Cuts at: 0 604 739 990
Size: 604 135 251
Fragments arranged by size:
604 251 135
SfcI C'TryA_G
Cuts at: 0 421 634 990
Size: 421 213 356
Fragments arranged by size:
421 356 213
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 826 990
Size: 826 164
SfoI GGC'GCC
Cuts at: 0 896 990
Size: 896 94
SmlI C'TyrA_G
Cuts at: 0 625 990
Size: 625 365
StuI AGG'CCT
Cuts at: 0 799 990
Size: 799 191
StyI C'CwwG_G
Cuts at: 0 95 176 499 990
Size: 95 81 323 491
Fragments arranged by size:
491 323 95 81
TatI w'GTAC_w
Cuts at: 0 602 737 990
Size: 602 135 253
Fragments arranged by size:
602 253 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 990
Size: 748 32 210
Fragments arranged by size:
748 210 32
Tth111I GACn'n_nGTC
Cuts at: 0 790 990
Size: 790 200
Enzymes that do cut:
AcuI AflIII AgeI ApaI ApaLI BamHI BanI BanII
BbeI BcgI BglI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PasI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SfiI SfoI SmlI StuI StyI TatI
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI Bpu10I BsaBI BsaXI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoNI
EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII XbaI XcmI XhoI XmaI
XmnI ZraI