Restriction map for
pF1KE1012
(Linear) (Six-base) MAPSORT of: KIEE1012.seq Check: 1423 from: 1 to: 906
KIEE1012.orf
With 182 enzymes: SgfI *
March 29, 2011 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 645 681 906
Size: 645 36 225
Fragments arranged by size:
645 225 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 719 827 906
Size: 719 108 79
Fragments arranged by size:
719 108 79
AlwNI CAG_nnn'CTG
Cuts at: 0 390 515 906
Size: 390 125 391
Fragments arranged by size:
391 390 125
ApaI G_GGCC'C
Cuts at: 0 57 906
Size: 57 849
ApoI r'AATT_y
Cuts at: 0 181 906
Size: 181 725
AvaI C'yCGr_G
Cuts at: 0 63 76 300 906
Size: 63 13 224 606
Fragments arranged by size:
606 224 63 13
BanI G'GyrC_C
Cuts at: 0 593 906
Size: 593 313
BanII G_rGCy'C
Cuts at: 0 57 352 906
Size: 57 295 554
Fragments arranged by size:
554 295 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 906
Size: 300 606
Bme1580I G_kGCm'C
Cuts at: 0 57 596 615 906
Size: 57 539 19 291
Fragments arranged by size:
539 291 57 19
BmgBI CAC'GTC
Cuts at: 0 819 906
Size: 819 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 717 906
Size: 190 527 189
Fragments arranged by size:
527 190 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 265 274 476 598 906
Size: 235 30 9 202 122 308
Fragments arranged by size:
308 235 202 122 30 9
Bpu10I CC'TnA_GC
Cuts at: 0 208 906
Size: 208 698
BsaI GGTCTCn'nnnn_
Cuts at: 0 432 526 906
Size: 432 94 380
Fragments arranged by size:
432 380 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 700 906
Size: 360 340 206
Fragments arranged by size:
360 340 206
BseYI C'CCAG_C
Cuts at: 0 364 536 540 621 906
Size: 364 172 4 81 285
Fragments arranged by size:
364 285 172 81 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 841 906
Size: 715 126 65
Fragments arranged by size:
715 126 65
BsiEI CG_ry'CG
Cuts at: 0 98 317 906
Size: 98 219 589
Fragments arranged by size:
589 219 98
BsiHKAI G_wGCw'C
Cuts at: 0 703 906
Size: 703 203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 101 906
Size: 101 805
Bsp1286I G_dGCh'C
Cuts at: 0 57 352 596 615 703 906
Size: 57 295 244 19 88 203
Fragments arranged by size:
295 244 203 88 57 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 645 681 906
Size: 645 36 225
Fragments arranged by size:
645 225 36
BsrBI CCG'CTC
Cuts at: 0 90 906
Size: 90 816
BsrFI r'CCGG_y
Cuts at: 0 97 316 639 906
Size: 97 219 323 267
Fragments arranged by size:
323 267 219 97
BssHII G'CGCG_C
Cuts at: 0 31 113 906
Size: 31 82 793
Fragments arranged by size:
793 82 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 364 795 906
Size: 364 431 111
Fragments arranged by size:
431 364 111
BtgI C'CryG_G
Cuts at: 0 273 782 906
Size: 273 509 124
Fragments arranged by size:
509 273 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 534 824 906
Size: 534 290 82
Fragments arranged by size:
534 290 82
BtsI GCAGTG_nn'
Cuts at: 0 701 906
Size: 701 205
DrdI GACnn_nn'nnGTC
Cuts at: 0 676 906
Size: 676 230
EaeI y'GGCC_r
Cuts at: 0 95 314 731 785 906
Size: 95 219 417 54 121
Fragments arranged by size:
417 219 121 95 54
EagI C'GGCC_G
Cuts at: 0 95 314 906
Size: 95 219 592
Fragments arranged by size:
592 219 95
EarI CTCTTCn'nnn_
Cuts at: 0 637 906
Size: 637 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 119 420 514 619 658 906
Size: 96 23 301 94 105 39 248
Fragments arranged by size:
301 248 105 96 94 39 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 265 274 476 598 906
Size: 235 30 9 202 122 308
Fragments arranged by size:
308 235 202 122 30 9
EcoO109I rG'GnC_Cy
Cuts at: 0 53 648 906
Size: 53 595 258
Fragments arranged by size:
595 258 53
FseI GG_CCGG'CC
Cuts at: 0 320 906
Size: 320 586
HaeII r_GCGC'y
Cuts at: 0 7 123 906
Size: 7 116 783
Fragments arranged by size:
783 116 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 906
Size: 437 469
MscI TGG'CCA
Cuts at: 0 733 906
Size: 733 173
MspA1I CmG'CkG
Cuts at: 0 135 275 454 540 621 756 906
Size: 135 140 179 86 81 135 150
Fragments arranged by size:
179 150 140 135 135 86 81
NaeI GCC'GGC
Cuts at: 0 99 318 641 906
Size: 99 219 323 265
Fragments arranged by size:
323 265 219 99
NgoMIV G'CCGG_C
Cuts at: 0 97 316 639 906
Size: 97 219 323 267
Fragments arranged by size:
323 267 219 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 906
Size: 729 177
PfoI T'CCnGG_A
Cuts at: 0 680 906
Size: 680 226
PspOMI G'GGCC_C
Cuts at: 0 53 906
Size: 53 853
PstI C_TGCA'G
Cuts at: 0 620 656 872 906
Size: 620 36 216 34
Fragments arranged by size:
620 216 36 34
PvuII CAG'CTG
Cuts at: 0 540 621 756 906
Size: 540 81 135 150
Fragments arranged by size:
540 150 135 81
RsrII CG'GwC_CG
Cuts at: 0 294 906
Size: 294 612
SacII CC_GC'GG
Cuts at: 0 276 906
Size: 276 630
SapI GCTCTTCn'nnn_
Cuts at: 0 637 906
Size: 637 269
SbfI CC_TGCA'GG
Cuts at: 0 656 906
Size: 656 250
SexAI A'CCwGG_T
Cuts at: 0 416 671 906
Size: 416 255 235
Fragments arranged by size:
416 255 235
SfcI C'TryA_G
Cuts at: 0 616 652 868 906
Size: 616 36 216 38
Fragments arranged by size:
616 216 38 36
SgrAI Cr'CCGG_yG
Cuts at: 0 639 906
Size: 639 267
SmaI CCC'GGG
Cuts at: 0 65 302 906
Size: 65 237 604
Fragments arranged by size:
604 237 65
StyI C'CwwG_G
Cuts at: 0 761 906
Size: 761 145
TatI w'GTAC_w
Cuts at: 0 875 906
Size: 875 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 420 906
Size: 420 486
XmaI C'CCGG_G
Cuts at: 0 63 300 906
Size: 63 237 606
Fragments arranged by size:
606 237 63
Enzymes that do cut:
AarI AlfI AlwNI ApaI ApoI AvaI BanI BanII
BbsI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstXI BtgI BtgZI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FseI HaeII MmeI
MscI MspA1I NaeI NgoMIV PflMI PfoI PspOMI PstI
PvuII RsrII SacII SapI SbfI SexAI SfcI SgrAI
SmaI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI SacI SalI SanDI ScaI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI