(Linear) (Six-base) MAPSORT of: KIEE1011.seq  Check: 1450  from: 1  to: 684



KIEE1011.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     128     684 
   Size:         78      50     556

  Fragments arranged by size: 

                 556      78      50
 
AflIII A'CryG_T

Cuts at:      0     656     684 
   Size:        656      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0     128     484     684 
   Size:        128     356     200

  Fragments arranged by size: 

                 356     200     128
 
AvaI C'yCGr_G

Cuts at:      0     600     684 
   Size:        600      84
 
BanI G'GyrC_C

Cuts at:      0     145     684 
   Size:        145     539
 
BanII G_rGCy'C

Cuts at:      0      25     628     684 
   Size:         25     603      56

  Fragments arranged by size: 

                 603      56      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     684 
   Size:        549     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     414     684 
   Size:        414     270
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     610     640     684 
   Size:        610      30      44

  Fragments arranged by size: 

                 610      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     525     618     684 
   Size:        525      93      66

  Fragments arranged by size: 

                 525      93      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     449     590     684 
   Size:         72     377     141      94

  Fragments arranged by size: 

                 377     141      94      72
 
BsiHKAI G_wGCw'C

Cuts at:      0     463     616     684 
   Size:        463     153      68

  Fragments arranged by size: 

                 463     153      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     463     616     628     684 
   Size:         25     438     153      12      56

  Fragments arranged by size: 

                 438     153      56      25      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60     684 
   Size:         60     624
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     128     684 
   Size:        128     556
 
BstYI r'GATC_y

Cuts at:      0     357     684 
   Size:        357     327
 
BtgI C'CryG_G

Cuts at:      0       5     684 
   Size:          5     679
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     146     684 
   Size:        146     538
 
BtsI GCAGTG_nn'

Cuts at:      0     461     684 
   Size:        461     223
 
DraI TTT'AAA

Cuts at:      0     117     684 
   Size:        117     567
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     684 
   Size:         13     671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     128     549     684 
   Size:         78      50     421     135

  Fragments arranged by size: 

                 421     135      78      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0     434     684 
   Size:        434     250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     610     642     684 
   Size:        159      32     419      32      42

  Fragments arranged by size: 

                 419     159      42      32      32
 
MspA1I CmG'CkG

Cuts at:      0     180     684 
   Size:        180     504
 
NcoI C'CATG_G

Cuts at:      0       5     684 
   Size:          5     679
 
NspI r_CATG'y

Cuts at:      0     660     684 
   Size:        660      24
 
PciI A'CATG_T

Cuts at:      0     656     684 
   Size:        656      28
 
PvuII CAG'CTG

Cuts at:      0     180     684 
   Size:        180     504
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13     684 
   Size:         13     671
 
SmlI C'TyrA_G

Cuts at:      0     429     684 
   Size:        429     255
 
StyI C'CwwG_G

Cuts at:      0       5     684 
   Size:          5     679
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     AvaI     BanI    BanII     BpmI    BpuEI
    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstYI
     BtgI    BtgZI     BtsI     DraI     EarI  Eco57MI EcoO109I    Hin4I
   MspA1I     NcoI     NspI     PciI    PvuII     SapI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI