Restriction map for
pF1KE1007
(Linear) (Six-base) MAPSORT of: KIEE1007.seq Check: 4194 from: 1 to: 588
KIEE1007.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 342 423 588
Size: 342 81 165
Fragments arranged by size:
342 165 81
AatII G_ACGT'C
Cuts at: 0 371 588
Size: 371 217
AccI GT'mk_AC
Cuts at: 0 206 588
Size: 206 382
AhdI GACnn_n'nnGTC
Cuts at: 0 558 588
Size: 558 30
AlwNI CAG_nnn'CTG
Cuts at: 0 363 588
Size: 363 225
AvaI C'yCGr_G
Cuts at: 0 505 588
Size: 505 83
BanI G'GyrC_C
Cuts at: 0 511 588
Size: 511 77
BanII G_rGCy'C
Cuts at: 0 403 535 588
Size: 403 132 53
Fragments arranged by size:
403 132 53
BbeI G_GCGC'C
Cuts at: 0 515 588
Size: 515 73
BglI GCCn_nnn'nGGC
Cuts at: 0 61 588
Size: 61 527
BlpI GC'TnA_GC
Cuts at: 0 493 588
Size: 493 95
Bme1580I G_kGCm'C
Cuts at: 0 135 588
Size: 135 453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 531 588
Size: 90 441 57
Fragments arranged by size:
441 90 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 496 588
Size: 64 432 92
Fragments arranged by size:
432 92 64
Bpu10I CC'TnA_GC
Cuts at: 0 298 588
Size: 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 327 588
Size: 327 261
BsaHI Gr'CG_yC
Cuts at: 0 196 368 512 588
Size: 196 172 144 76
Fragments arranged by size:
196 172 144 76
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 315 478 588
Size: 315 163 110
Fragments arranged by size:
315 163 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 376 588
Size: 247 129 212
Fragments arranged by size:
247 212 129
BsiHKAI G_wGCw'C
Cuts at: 0 304 403 535 588
Size: 304 99 132 53
Fragments arranged by size:
304 132 99 53
Bsp1286I G_dGCh'C
Cuts at: 0 135 304 403 535 588
Size: 135 169 99 132 53
Fragments arranged by size:
169 135 132 99 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 342 423 588
Size: 320 22 81 165
Fragments arranged by size:
320 165 81 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 588
Size: 354 234
BstEII G'GTnAC_C
Cuts at: 0 252 273 588
Size: 252 21 315
Fragments arranged by size:
315 252 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 285 588
Size: 264 21 303
Fragments arranged by size:
303 264 21
BtgI C'CryG_G
Cuts at: 0 305 521 588
Size: 305 216 67
Fragments arranged by size:
305 216 67
BtsI GCAGTG_nn'
Cuts at: 0 218 588
Size: 218 370
EaeI y'GGCC_r
Cuts at: 0 53 404 572 588
Size: 53 351 168 16
Fragments arranged by size:
351 168 53 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 97 588
Size: 79 18 491
Fragments arranged by size:
491 79 18
EcoICRI GAG'CTC
Cuts at: 0 401 533 588
Size: 401 132 55
Fragments arranged by size:
401 132 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 496 588
Size: 64 432 92
Fragments arranged by size:
432 92 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 316 588
Size: 289 27 272
Fragments arranged by size:
289 272 27
EcoO109I rG'GnC_Cy
Cuts at: 0 105 294 588
Size: 105 189 294
Fragments arranged by size:
294 189 105
FspI TGC'GCA
Cuts at: 0 541 588
Size: 541 47
HaeII r_GCGC'y
Cuts at: 0 515 588
Size: 515 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 588
Size: 544 32 12
Fragments arranged by size:
544 32 12
KasI G'GCGC_C
Cuts at: 0 511 588
Size: 511 77
MscI TGG'CCA
Cuts at: 0 55 588
Size: 55 533
MspA1I CmG'CkG
Cuts at: 0 42 235 501 558 588
Size: 42 193 266 57 30
Fragments arranged by size:
266 193 57 42 30
NarI GG'CG_CC
Cuts at: 0 512 588
Size: 512 76
NcoI C'CATG_G
Cuts at: 0 305 588
Size: 305 283
PstI C_TGCA'G
Cuts at: 0 116 317 588
Size: 116 201 271
Fragments arranged by size:
271 201 116
PvuII CAG'CTG
Cuts at: 0 42 235 501 558 588
Size: 42 193 266 57 30
Fragments arranged by size:
266 193 57 42 30
SacI G_AGCT'C
Cuts at: 0 403 535 588
Size: 403 132 53
Fragments arranged by size:
403 132 53
SfcI C'TryA_G
Cuts at: 0 112 313 588
Size: 112 201 275
Fragments arranged by size:
275 201 112
SfoI GGC'GCC
Cuts at: 0 513 588
Size: 513 75
SmlI C'TyrA_G
Cuts at: 0 579 588
Size: 579 9
StyI C'CwwG_G
Cuts at: 0 77 221 305 588
Size: 77 144 84 283
Fragments arranged by size:
283 144 84 77
ZraI GAC'GTC
Cuts at: 0 369 588
Size: 369 219
Enzymes that do cut:
AarI AatII AccI AhdI AlwNI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaHI BseRI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstEII
BstXI BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MscI MspA1I NarI
NcoI PstI PvuII SacI SfcI SfoI SmlI StyI
ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI
BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI