(Linear) (Six-base) MAPSORT of: KIEE1001.seq  Check: 1232  from: 1  to: 399



KIEE1001.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

Acc65I G'GTAC_C

Cuts at:      0      32     399 
   Size:         32     367
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     352     399 
   Size:        182     170      47

  Fragments arranged by size: 

                 182     170      47
 
AflIII A'CryG_T

Cuts at:      0     188     399 
   Size:        188     211
 
ApaI G_GGCC'C

Cuts at:      0     138     399 
   Size:        138     261
 
AvaI C'yCGr_G

Cuts at:      0      10     399 
   Size:         10     389
 
BanI G'GyrC_C

Cuts at:      0      32     399 
   Size:         32     367
 
BanII G_rGCy'C

Cuts at:      0      56     138     399 
   Size:         56      82     261

  Fragments arranged by size: 

                 261      82      56
 
Bme1580I G_kGCm'C

Cuts at:      0     138     399 
   Size:        138     261
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     399 
   Size:        364      35
 
BsaBI GATnn'nnATC

Cuts at:      0     242     399 
   Size:        242     157
 
BsaWI w'CCGG_w

Cuts at:      0     324     399 
   Size:        324      75
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      76     399 
   Size:         64      12     323

  Fragments arranged by size: 

                 323      64      12
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     399 
   Size:         56     343
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     138     399 
   Size:         56      82     261

  Fragments arranged by size: 

                 261      82      56
 
BstYI r'GATC_y

Cuts at:      0     147     399 
   Size:        147     252
 
Bsu36I CC'TnA_GG

Cuts at:      0     130     399 
   Size:        130     269
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      88     399 
   Size:         88     311
 
EcoICRI GAG'CTC

Cuts at:      0      54     399 
   Size:         54     345
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     352     399 
   Size:        182     170      47

  Fragments arranged by size: 

                 182     170      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     142     307     358     399 
   Size:        142     165      51      41

  Fragments arranged by size: 

                 165     142      51      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     126     134     135     399 
   Size:        126       8       1     264

  Fragments arranged by size: 

                 264     126       8       1
 
HincII GTy'rAC

Cuts at:      0     297     399 
   Size:        297     102
 
KpnI G_GTAC'C

Cuts at:      0      36     399 
   Size:         36     363
 
NspI r_CATG'y

Cuts at:      0     192     399 
   Size:        192     207
 
PciI A'CATG_T

Cuts at:      0     188     399 
   Size:        188     211
 
PspOMI G'GGCC_C

Cuts at:      0     134     399 
   Size:        134     265
 
PstI C_TGCA'G

Cuts at:      0     143     399 
   Size:        143     256
 
SacI G_AGCT'C

Cuts at:      0      56     399 
   Size:         56     343
 
SfcI C'TryA_G

Cuts at:      0     139     399 
   Size:        139     260
 
SmlI C'TyrA_G

Cuts at:      0     379     399 
   Size:        379      20
 
StyI C'CwwG_G

Cuts at:      0     315     399 
   Size:        315      84
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     ApaI     AvaI     BanI    BanII Bme1580I
    BpuEI    BsaBI    BsaWI    BseRI  BsiHKAI Bsp1286I    BstYI   Bsu36I
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I   HincII     KpnI     NspI
     PciI   PspOMI     PstI     SacI     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI