Restriction map for
pF1KE0986
(Linear) (Six-base) MAPSORT of: KIEE0986.seq Check: 2277 from: 1 to: 1803
KIEE0986.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 239 624 693 824 1803
Size: 239 385 69 131 979
Fragments arranged by size:
979 385 239 131 69
AccI GT'mk_AC
Cuts at: 0 1136 1803
Size: 1136 667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 942 1519 1803
Size: 64 878 577 284
Fragments arranged by size:
878 577 284 64
AfeI AGC'GCT
Cuts at: 0 772 1803
Size: 772 1031
AflII C'TTAA_G
Cuts at: 0 1045 1803
Size: 1045 758
AflIII A'CryG_T
Cuts at: 0 1139 1803
Size: 1139 664
AgeI A'CCGG_T
Cuts at: 0 1669 1803
Size: 1669 134
AlwNI CAG_nnn'CTG
Cuts at: 0 1332 1803
Size: 1332 471
ApaI G_GGCC'C
Cuts at: 0 593 1468 1803
Size: 593 875 335
Fragments arranged by size:
875 593 335
ApoI r'AATT_y
Cuts at: 0 867 946 1359 1803
Size: 867 79 413 444
Fragments arranged by size:
867 444 413 79
AvaI C'yCGr_G
Cuts at: 0 90 1051 1803
Size: 90 961 752
Fragments arranged by size:
961 752 90
BamHI G'GATC_C
Cuts at: 0 1554 1803
Size: 1554 249
BanI G'GyrC_C
Cuts at: 0 363 636 1592 1803
Size: 363 273 956 211
Fragments arranged by size:
956 363 273 211
BanII G_rGCy'C
Cuts at: 0 98 409 593 932 1170 1244 1372 1468
Size: 98 311 184 339 238 74 128 96
Cuts at: 1468 1803
Size: 335
Fragments arranged by size:
339 335 311 238 184 128 98 96
74
BbvCI CC'TCA_GC
Cuts at: 0 1603 1803
Size: 1603 200
BclI T'GATC_A
Cuts at: 0 225 1803
Size: 225 1578
BfrBI ATG'CAT
Cuts at: 0 1342 1803
Size: 1342 461
BglI GCCn_nnn'nGGC
Cuts at: 0 372 1803
Size: 372 1431
BlpI GC'TnA_GC
Cuts at: 0 910 1713 1803
Size: 910 803 90
Fragments arranged by size:
910 803 90
Bme1580I G_kGCm'C
Cuts at: 0 593 641 1468 1803
Size: 593 48 827 335
Fragments arranged by size:
827 593 335 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1095 1154 1803
Size: 883 212 59 649
Fragments arranged by size:
883 649 212 59
Bpu10I CC'TnA_GC
Cuts at: 0 1603 1803
Size: 1603 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1803
Size: 658 1145
BsaAI yAC'GTr
Cuts at: 0 651 741 1142 1803
Size: 651 90 401 661
Fragments arranged by size:
661 651 401 90
BsaBI GATnn'nnATC
Cuts at: 0 802 1803
Size: 802 1001
BsaWI w'CCGG_w
Cuts at: 0 1470 1669 1803
Size: 1470 199 134
Fragments arranged by size:
1470 199 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1627 1657 1803
Size: 1627 30 146
Fragments arranged by size:
1627 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 808 1620 1803
Size: 808 812 183
Fragments arranged by size:
812 808 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 652 1377 1803
Size: 122 530 725 426
Fragments arranged by size:
725 530 426 122
BsiEI CG_ry'CG
Cuts at: 0 23 47 51 1803
Size: 23 24 4 1752
Fragments arranged by size:
1752 24 23 4
BsiHKAI G_wGCw'C
Cuts at: 0 311 409 932 1244 1372 1669 1803
Size: 311 98 523 312 128 297 134
Fragments arranged by size:
523 312 311 297 134 128 98
BsmI GAATG_Cn'
Cuts at: 0 386 1803
Size: 386 1417
Bsp1286I G_dGCh'C
Cuts at: 0 98 311 409 593 641 932 1170 1244
Size: 98 213 98 184 48 291 238 74
Cuts at: 1244 1372 1468 1669 1803
Size: 128 96 201 134
Fragments arranged by size:
291 238 213 201 184 134 128 98
98 96 74 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 624 693 824 1803
Size: 239 385 69 131 979
Fragments arranged by size:
979 385 239 131 69
BsrBI CCG'CTC
Cuts at: 0 28 119 372 1803
Size: 28 91 253 1431
Fragments arranged by size:
1431 253 91 28
BsrDI GCAATG_nn'
Cuts at: 0 482 1803
Size: 482 1321
BsrFI r'CCGG_y
Cuts at: 0 46 779 1669 1803
Size: 46 733 890 134
Fragments arranged by size:
890 733 134 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 1803
Size: 489 1314
BstBI TT'CG_AA
Cuts at: 0 509 1803
Size: 509 1294
BstEII G'GTnAC_C
Cuts at: 0 1252 1803
Size: 1252 551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 199 1803
Size: 199 1604
BstYI r'GATC_y
Cuts at: 0 260 797 1554 1803
Size: 260 537 757 249
Fragments arranged by size:
757 537 260 249
BtgI C'CryG_G
Cuts at: 0 425 1803
Size: 425 1378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 132 1803
Size: 132 1671
BtsI GCAGTG_nn'
Cuts at: 0 1006 1568 1803
Size: 1006 562 235
Fragments arranged by size:
1006 562 235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 1803
Size: 582 35 1186
Fragments arranged by size:
1186 582 35
EaeI y'GGCC_r
Cuts at: 0 20 48 1803
Size: 20 28 1755
Fragments arranged by size:
1755 28 20
EagI C'GGCC_G
Cuts at: 0 20 48 1803
Size: 20 28 1755
Fragments arranged by size:
1755 28 20
EcoICRI GAG'CTC
Cuts at: 0 407 930 1242 1370 1803
Size: 407 523 312 128 433
Fragments arranged by size:
523 433 407 312 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 883 942 1095 1154 1519 1803
Size: 64 819 59 153 59 365 284
Fragments arranged by size:
819 365 284 153 64 59 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1602 1803
Size: 1602 201
EcoO109I rG'GnC_Cy
Cuts at: 0 669 1464 1803
Size: 669 795 339
Fragments arranged by size:
795 669 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1198 1803
Size: 711 487 605
Fragments arranged by size:
711 605 487
FspI TGC'GCA
Cuts at: 0 1567 1803
Size: 1567 236
FspAI rTGC'GCAy
Cuts at: 0 1567 1803
Size: 1567 236
HaeII r_GCGC'y
Cuts at: 0 68 573 774 1803
Size: 68 505 201 1029
Fragments arranged by size:
1029 505 201 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1093 1125 1627 1659 1803
Size: 607 32 454 32 502 32 144
Fragments arranged by size:
607 502 454 144 32 32 32
HincII GTy'rAC
Cuts at: 0 5 1803
Size: 5 1798
HpaI GTT'AAC
Cuts at: 0 5 1803
Size: 5 1798
MslI CAynn'nnrTG
Cuts at: 0 233 457 856 892 1130 1210 1803
Size: 233 224 399 36 238 80 593
Fragments arranged by size:
593 399 238 233 224 80 36
MspA1I CmG'CkG
Cuts at: 0 378 790 1290 1803
Size: 378 412 500 513
Fragments arranged by size:
513 500 412 378
NcoI C'CATG_G
Cuts at: 0 425 1803
Size: 425 1378
NdeI CA'TA_TG
Cuts at: 0 627 857 1803
Size: 627 230 946
Fragments arranged by size:
946 627 230
NsiI A_TGCA'T
Cuts at: 0 1344 1803
Size: 1344 459
NspI r_CATG'y
Cuts at: 0 238 492 1203 1320 1803
Size: 238 254 711 117 483
Fragments arranged by size:
711 483 254 238 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 861 1803
Size: 741 120 942
Fragments arranged by size:
942 741 120
PfoI T'CCnGG_A
Cuts at: 0 806 1444 1803
Size: 806 638 359
Fragments arranged by size:
806 638 359
PmlI CAC'GTG
Cuts at: 0 651 741 1803
Size: 651 90 1062
Fragments arranged by size:
1062 651 90
PpuMI rG'GwC_Cy
Cuts at: 0 669 1803
Size: 669 1134
PspOMI G'GGCC_C
Cuts at: 0 589 1464 1803
Size: 589 875 339
Fragments arranged by size:
875 589 339
PstI C_TGCA'G
Cuts at: 0 1001 1563 1803
Size: 1001 562 240
Fragments arranged by size:
1001 562 240
PvuII CAG'CTG
Cuts at: 0 378 1290 1803
Size: 378 912 513
Fragments arranged by size:
912 513 378
SacI G_AGCT'C
Cuts at: 0 409 932 1244 1372 1803
Size: 409 523 312 128 431
Fragments arranged by size:
523 431 409 312 128
SfcI C'TryA_G
Cuts at: 0 997 1559 1803
Size: 997 562 244
Fragments arranged by size:
997 562 244
SgrAI Cr'CCGG_yG
Cuts at: 0 1669 1803
Size: 1669 134
SmaI CCC'GGG
Cuts at: 0 92 1803
Size: 92 1711
SmlI C'TyrA_G
Cuts at: 0 673 1045 1803
Size: 673 372 758
Fragments arranged by size:
758 673 372
SphI G_CATG'C
Cuts at: 0 1203 1320 1803
Size: 1203 117 483
Fragments arranged by size:
1203 483 117
SspI AAT'ATT
Cuts at: 0 1767 1803
Size: 1767 36
StuI AGG'CCT
Cuts at: 0 715 1803
Size: 715 1088
StyI C'CwwG_G
Cuts at: 0 425 1182 1373 1803
Size: 425 757 191 430
Fragments arranged by size:
757 430 425 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 1803
Size: 614 32 1157
Fragments arranged by size:
1157 614 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 1803
Size: 432 1371
XmaI C'CCGG_G
Cuts at: 0 90 1803
Size: 90 1713
XmnI GAAnn'nnTTC
Cuts at: 0 772 946 1803
Size: 772 174 857
Fragments arranged by size:
857 772 174
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AgeI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbvCI BclI
BfrBI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII HpaI MslI MspA1I NcoI NdeI NsiI
NspI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SfcI SgrAI SmaI SmlI SphI SspI StuI
StyI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AhdI AleI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI
BciVI BglII BmgBI BmrI BmtI BplI BsaI BsaHI
BseYI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EarI EciI
EcoRI EcoRV FseI HindIII KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI
SwaI TaqII TatI Tth111I XbaI XhoI ZraI