Restriction map for
pF1KE0983
(Linear) (Six-base) MAPSORT of: KIEE0983.seq Check: 3132 from: 1 to: 1764
KIEE0983.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 654 1764
Size: 654 1110
AccI GT'mk_AC
Cuts at: 0 1728 1764
Size: 1728 36
AclI AA'CG_TT
Cuts at: 0 809 1764
Size: 809 955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1027 1764
Size: 460 567 737
Fragments arranged by size:
737 567 460
AfeI AGC'GCT
Cuts at: 0 733 1764
Size: 733 1031
AflII C'TTAA_G
Cuts at: 0 778 1764
Size: 778 986
AhdI GACnn_n'nnGTC
Cuts at: 0 1068 1605 1764
Size: 1068 537 159
Fragments arranged by size:
1068 537 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 1764
Size: 1762 2
ApoI r'AATT_y
Cuts at: 0 271 500 1764
Size: 271 229 1264
Fragments arranged by size:
1264 271 229
AseI AT'TA_AT
Cuts at: 0 488 1328 1764
Size: 488 840 436
Fragments arranged by size:
840 488 436
AvaI C'yCGr_G
Cuts at: 0 602 1764
Size: 602 1162
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 925 958 1764
Size: 925 33 806
Fragments arranged by size:
925 806 33
BanI G'GyrC_C
Cuts at: 0 402 414 1764
Size: 402 12 1350
Fragments arranged by size:
1350 402 12
BanII G_rGCy'C
Cuts at: 0 59 1131 1764
Size: 59 1072 633
Fragments arranged by size:
1072 633 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 186 1498 1764
Size: 186 1312 266
Fragments arranged by size:
1312 266 186
BbvCI CC'TCA_GC
Cuts at: 0 752 1764
Size: 752 1012
BclI T'GATC_A
Cuts at: 0 681 1764
Size: 681 1083
BlpI GC'TnA_GC
Cuts at: 0 871 1764
Size: 871 893
BmrI ACTGGGnnnn_n'
Cuts at: 0 942 1455 1521 1764
Size: 942 513 66 243
Fragments arranged by size:
942 513 243 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1115 1250 1318 1652 1764
Size: 410 705 135 68 334 112
Fragments arranged by size:
705 410 334 135 112 68
Bpu10I CC'TnA_GC
Cuts at: 0 752 1764
Size: 752 1012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1526 1764
Size: 1526 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 599 1238 1764
Size: 599 639 526
Fragments arranged by size:
639 599 526
BsaAI yAC'GTr
Cuts at: 0 702 1408 1764
Size: 702 706 356
Fragments arranged by size:
706 702 356
BsaWI w'CCGG_w
Cuts at: 0 761 839 1764
Size: 761 78 925
Fragments arranged by size:
925 761 78
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 1764
Size: 769 995
BseYI C'CCAG_C
Cuts at: 0 65 1536 1764
Size: 65 1471 228
Fragments arranged by size:
1471 228 65
BsiHKAI G_wGCw'C
Cuts at: 0 1131 1764
Size: 1131 633
Bsp1286I G_dGCh'C
Cuts at: 0 59 1131 1764
Size: 59 1072 633
Fragments arranged by size:
1072 633 59
BspEI T'CCGG_A
Cuts at: 0 761 839 1764
Size: 761 78 925
Fragments arranged by size:
925 761 78
BspHI T'CATG_A
Cuts at: 0 104 1764
Size: 104 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 654 1764
Size: 654 1110
BsrDI GCAATG_nn'
Cuts at: 0 729 1764
Size: 729 1035
BssSI C'ACGA_G
Cuts at: 0 14 1764
Size: 14 1750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1375 1764
Size: 1375 389
BstYI r'GATC_y
Cuts at: 0 758 1764
Size: 758 1006
BstZ17I GTA'TAC
Cuts at: 0 1729 1764
Size: 1729 35
BtgI C'CryG_G
Cuts at: 0 1035 1764
Size: 1035 729
BtsI GCAGTG_nn'
Cuts at: 0 461 996 1746 1764
Size: 461 535 750 18
Fragments arranged by size:
750 535 461 18
ClaI AT'CG_AT
Cuts at: 0 621 1764
Size: 621 1143
EaeI y'GGCC_r
Cuts at: 0 668 1764
Size: 668 1096
EarI CTCTTCn'nnn_
Cuts at: 0 1228 1764
Size: 1228 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 1764
Size: 42 1722
EcoICRI GAG'CTC
Cuts at: 0 1129 1764
Size: 1129 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 460 1027 1115 1250 1318 1652 1764
Size: 410 50 567 88 135 68 334 112
Fragments arranged by size:
567 410 334 135 112 88 68 50
EcoO109I rG'GnC_Cy
Cuts at: 0 584 1426 1515 1764
Size: 584 842 89 249
Fragments arranged by size:
842 584 249 89
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1574 1764
Size: 1574 190
FspI TGC'GCA
Cuts at: 0 721 1764
Size: 721 1043
HaeII r_GCGC'y
Cuts at: 0 735 1764
Size: 735 1029
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1205 1237 1524 1556 1591 1623
Size: 1054 32 119 32 287 32 35 32
Cuts at: 1623 1764
Size: 141
Fragments arranged by size:
1054 287 141 119 35 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1690 1764
Size: 1690 74
MfeI C'AATT_G
Cuts at: 0 1166 1764
Size: 1166 598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1656 1764
Size: 410 1246 108
Fragments arranged by size:
1246 410 108
MscI TGG'CCA
Cuts at: 0 670 1764
Size: 670 1094
MslI CAynn'nnrTG
Cuts at: 0 103 1764
Size: 103 1661
MspA1I CmG'CkG
Cuts at: 0 72 261 751 1076 1764
Size: 72 189 490 325 688
Fragments arranged by size:
688 490 325 189 72
NdeI CA'TA_TG
Cuts at: 0 1174 1764
Size: 1174 590
NspI r_CATG'y
Cuts at: 0 822 1764
Size: 822 942
PflMI CCAn_nnn'nTGG
Cuts at: 0 822 1650 1675 1764
Size: 822 828 25 89
Fragments arranged by size:
828 822 89 25
PpuMI rG'GwC_Cy
Cuts at: 0 1426 1515 1764
Size: 1426 89 249
Fragments arranged by size:
1426 249 89
PvuII CAG'CTG
Cuts at: 0 261 751 1076 1764
Size: 261 490 325 688
Fragments arranged by size:
688 490 325 261
SacI G_AGCT'C
Cuts at: 0 1131 1764
Size: 1131 633
SanDI GG'GwC_CC
Cuts at: 0 1426 1515 1764
Size: 1426 89 249
Fragments arranged by size:
1426 249 89
ScaI AGT'ACT
Cuts at: 0 1321 1764
Size: 1321 443
SfcI C'TryA_G
Cuts at: 0 1122 1155 1764
Size: 1122 33 609
Fragments arranged by size:
1122 609 33
SmlI C'TyrA_G
Cuts at: 0 602 778 1541 1764
Size: 602 176 763 223
Fragments arranged by size:
763 602 223 176
StyI C'CwwG_G
Cuts at: 0 398 1052 1334 1558 1764
Size: 398 654 282 224 206
Fragments arranged by size:
654 398 282 224 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 420 1764
Size: 189 231 1344
Fragments arranged by size:
1344 231 189
TatI w'GTAC_w
Cuts at: 0 144 1319 1764
Size: 144 1175 445
Fragments arranged by size:
1175 445 144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1172 1764
Size: 1172 592
XhoI C'TCGA_G
Cuts at: 0 602 1764
Size: 602 1162
XmnI GAAnn'nnTTC
Cuts at: 0 1019 1764
Size: 1019 745
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AhdI AlfI
ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI
BclI BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BssSI BstXI BstYI BstZ17I BtgI BtsI ClaI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HindIII MfeI MmeI MscI MslI MspA1I
NdeI NspI PflMI PpuMI PvuII SacI SanDI ScaI
SfcI SmlI StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AflIII AgeI AleI AloI AlwNI ApaI
ApaLI AscI AsiSI AvrII BamHI BbeI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BsaBI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII Bsu36I BtgZI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV
FseI FspAI HincII HpaI KasI KpnI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XmaI ZraI