(Linear) (Six-base) MAPSORT of: KIEE0964.seq  Check: 5760  from: 1  to: 1524



KIEE0964.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

Acc65I G'GTAC_C

Cuts at:      0     406    1524 
   Size:        406    1118
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1524 
   Size:        781     743
 
AfeI AGC'GCT

Cuts at:      0    1107    1524 
   Size:       1107     417
 
AflIII A'CryG_T

Cuts at:      0     171    1524 
   Size:        171    1353
 
AlwNI CAG_nnn'CTG

Cuts at:      0     759     854    1524 
   Size:        759      95     670

  Fragments arranged by size: 

                 759     670      95
 
ApoI r'AATT_y

Cuts at:      0     223     296     328     932    1524 
   Size:        223      73      32     604     592

  Fragments arranged by size: 

                 604     592     223      73      32
 
BanI G'GyrC_C

Cuts at:      0     406    1524 
   Size:        406    1118
 
BanII G_rGCy'C

Cuts at:      0     812     837    1524 
   Size:        812      25     687

  Fragments arranged by size: 

                 812     687      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1203    1524 
   Size:       1203     321
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     451    1524 
   Size:        451    1073
 
BglII A'GATC_T

Cuts at:      0     389    1336    1524 
   Size:        389     947     188

  Fragments arranged by size: 

                 947     389     188
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     100     810    1419    1524 
   Size:        100     710     609     105

  Fragments arranged by size: 

                 710     609     105     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131    1524 
   Size:        131    1393
 
Bpu10I CC'TnA_GC

Cuts at:      0    1178    1524 
   Size:       1178     346
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910    1202    1524 
   Size:        910     292     322

  Fragments arranged by size: 

                 910     322     292
 
BsaBI GATnn'nnATC

Cuts at:      0    1341    1524 
   Size:       1341     183
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1156    1524 
   Size:       1156     368
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1153    1524 
   Size:        799     354     371

  Fragments arranged by size: 

                 799     371     354
 
BsiHKAI G_wGCw'C

Cuts at:      0     812     885    1524 
   Size:        812      73     639

  Fragments arranged by size: 

                 812     639      73
 
Bsp1286I G_dGCh'C

Cuts at:      0     812     837     885    1524 
   Size:        812      25      48     639

  Fragments arranged by size: 

                 812     639      48      25
 
BstYI r'GATC_y

Cuts at:      0     313     389     701    1336    1524 
   Size:        313      76     312     635     188

  Fragments arranged by size: 

                 635     313     312     188      76
 
DraI TTT'AAA

Cuts at:      0     414    1524 
   Size:        414    1110
 
EarI CTCTTCn'nnn_

Cuts at:      0    1436    1524 
   Size:       1436      88
 
EcoICRI GAG'CTC

Cuts at:      0     810    1524 
   Size:        810     714
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     781    1524 
   Size:        131     650     743

  Fragments arranged by size: 

                 743     650     131
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     620    1524 
   Size:        620     904
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765    1524 
   Size:        765     759
 
EcoRI G'AATT_C

Cuts at:      0     296     328    1524 
   Size:        296      32    1196

  Fragments arranged by size: 

                1196     296      32
 
HaeII r_GCGC'y

Cuts at:      0    1109    1524 
   Size:       1109     415
 
HincII GTy'rAC

Cuts at:      0     422    1056    1524 
   Size:        422     634     468

  Fragments arranged by size: 

                 634     468     422
 
KpnI G_GTAC'C

Cuts at:      0     410    1524 
   Size:        410    1114
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     782    1524 
   Size:        431     351     742

  Fragments arranged by size: 

                 742     431     351
 
MslI CAynn'nnrTG

Cuts at:      0     476     494    1524 
   Size:        476      18    1030

  Fragments arranged by size: 

                1030     476      18
 
NdeI CA'TA_TG

Cuts at:      0     897    1524 
   Size:        897     627
 
NspI r_CATG'y

Cuts at:      0     175     720    1524 
   Size:        175     545     804

  Fragments arranged by size: 

                 804     545     175
 
PasI CC'CwG_GG

Cuts at:      0     746    1524 
   Size:        746     778
 
PciI A'CATG_T

Cuts at:      0     171    1524 
   Size:        171    1353
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     873    1524 
   Size:        873     651
 
PpuMI rG'GwC_Cy

Cuts at:      0     765    1524 
   Size:        765     759
 
SacI G_AGCT'C

Cuts at:      0     812    1524 
   Size:        812     712
 
SexAI A'CCwGG_T

Cuts at:      0     539    1524 
   Size:        539     985
 
SfcI C'TryA_G

Cuts at:      0     570    1524 
   Size:        570     954
 
SmlI C'TyrA_G

Cuts at:      0     925    1217    1524 
   Size:        925     292     307

  Fragments arranged by size: 

                 925     307     292
 
SphI G_CATG'C

Cuts at:      0     720    1524 
   Size:        720     804
 
TatI w'GTAC_w

Cuts at:      0     456    1524 
   Size:        456    1068
 
XmnI GAAnn'nnTTC

Cuts at:      0     485    1524 
   Size:        485    1039
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI   AflIII    AlwNI     ApoI     BanI    BanII
     BbsI    BciVI    BglII     BmrI     BpmI   Bpu10I    BpuEI    BsaBI
    BseRI     BsgI  BsiHKAI Bsp1286I    BstYI     DraI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII   HincII     KpnI     MmeI
     MslI     NdeI     NspI     PasI     PciI    PflMI    PpuMI     SacI
    SexAI     SfcI     SmlI     SphI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII
     HpaI     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI