Restriction map for
pF1KE0963
(Linear) (Six-base) MAPSORT of: KIEE0963.seq Check: 524 from: 1 to: 1467
KIEE0963.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 320 673 1310 1450 1467
Size: 233 87 353 637 140 17
Fragments arranged by size:
637 353 233 140 87 17
AflII C'TTAA_G
Cuts at: 0 592 1204 1467
Size: 592 612 263
Fragments arranged by size:
612 592 263
AflIII A'CryG_T
Cuts at: 0 363 1467
Size: 363 1104
AhdI GACnn_n'nnGTC
Cuts at: 0 555 1419 1467
Size: 555 864 48
Fragments arranged by size:
864 555 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 993 1025 1467
Size: 677 32 284 32 442
Fragments arranged by size:
677 442 284 32 32
ApoI r'AATT_y
Cuts at: 0 135 515 846 933 1467
Size: 135 380 331 87 534
Fragments arranged by size:
534 380 331 135 87
AseI AT'TA_AT
Cuts at: 0 926 1467
Size: 926 541
AvaI C'yCGr_G
Cuts at: 0 758 799 1467
Size: 758 41 668
Fragments arranged by size:
758 668 41
BamHI G'GATC_C
Cuts at: 0 1344 1467
Size: 1344 123
BanII G_rGCy'C
Cuts at: 0 40 54 1467
Size: 40 14 1413
Fragments arranged by size:
1413 40 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 442 1467
Size: 308 134 1025
Fragments arranged by size:
1025 308 134
BfrBI ATG'CAT
Cuts at: 0 1081 1467
Size: 1081 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 1425 1467
Size: 1425 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1080 1467
Size: 494 586 387
Fragments arranged by size:
586 494 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1467
Size: 1378 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 1171 1467
Size: 1171 296
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1390 1408 1467
Size: 1390 18 59
Fragments arranged by size:
1390 59 18
BsiHKAI G_wGCw'C
Cuts at: 0 54 1467
Size: 54 1413
BsmI GAATG_Cn'
Cuts at: 0 1215 1467
Size: 1215 252
BsmBI CGTCTCn'nnnn_
Cuts at: 0 674 1467
Size: 674 793
Bsp1286I G_dGCh'C
Cuts at: 0 40 54 1467
Size: 40 14 1413
Fragments arranged by size:
1413 40 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1467
Size: 536 931
BstBI TT'CG_AA
Cuts at: 0 850 1467
Size: 850 617
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 518 1467
Size: 465 53 949
Fragments arranged by size:
949 465 53
BstYI r'GATC_y
Cuts at: 0 255 439 1344 1467
Size: 255 184 905 123
Fragments arranged by size:
905 255 184 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 715 1467
Size: 715 752
BtsI GCAGTG_nn'
Cuts at: 0 661 1171 1467
Size: 661 510 296
Fragments arranged by size:
661 510 296
DraI TTT'AAA
Cuts at: 0 714 1467
Size: 714 753
DrdI GACnn_nn'nnGTC
Cuts at: 0 13 1467
Size: 13 1454
EarI CTCTTCn'nnn_
Cuts at: 0 951 1467
Size: 951 516
EcoICRI GAG'CTC
Cuts at: 0 52 1467
Size: 52 1415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 320 494 673 1080 1310 1450 1467
Size: 233 87 174 179 407 230 140 17
Fragments arranged by size:
407 233 230 179 174 140 87 17
EcoO109I rG'GnC_Cy
Cuts at: 0 1279 1467
Size: 1279 188
EcoRI G'AATT_C
Cuts at: 0 135 1467
Size: 135 1332
EcoRV GAT'ATC
Cuts at: 0 381 1467
Size: 381 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 1467
Size: 414 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 424 456 541 573 1405 1437
Size: 370 32 22 32 85 32 832 32
Cuts at: 1437 1467
Size: 30
Fragments arranged by size:
832 370 85 32 32 32 32 30
22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1467
Size: 598 869
NsiI A_TGCA'T
Cuts at: 0 1083 1467
Size: 1083 384
PsiI TTA'TAA
Cuts at: 0 536 1467
Size: 536 931
SacI G_AGCT'C
Cuts at: 0 54 1467
Size: 54 1413
SexAI A'CCwGG_T
Cuts at: 0 1254 1467
Size: 1254 213
SmlI C'TyrA_G
Cuts at: 0 592 758 1204 1357 1467
Size: 592 166 446 153 110
Fragments arranged by size:
592 446 166 153 110
SspI AAT'ATT
Cuts at: 0 183 1467
Size: 183 1284
StuI AGG'CCT
Cuts at: 0 1454 1467
Size: 1454 13
StyI C'CwwG_G
Cuts at: 0 336 560 1467
Size: 336 224 907
Fragments arranged by size:
907 336 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1407 1467
Size: 1407 60
TatI w'GTAC_w
Cuts at: 0 1139 1467
Size: 1139 328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 334 1467
Size: 334 1133
XhoI C'TCGA_G
Cuts at: 0 758 1467
Size: 758 709
XmnI GAAnn'nnTTC
Cuts at: 0 397 1467
Size: 397 1070
Enzymes that do cut:
AcuI AflII AflIII AhdI AloI ApoI AseI AvaI
BamHI BanII BbsI BfrBI BmrI BpmI BpuEI BsaI
BseRI BsiHKAI BsmI BsmBI Bsp1286I BspMI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I MmeI NsiI PsiI
SacI SexAI SmlI SspI StuI StyI TaqII TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EaeI
EagI EciI EcoNI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XmaI
ZraI