Restriction map for
pF1KE0952
(Linear) (Six-base) MAPSORT of: KIEE0952.seq Check: 151 from: 1 to: 1422
KIEE0952.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 843 1422
Size: 843 579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 439 523 781 1162 1315 1422
Size: 275 164 84 258 381 153 107
Fragments arranged by size:
381 275 258 164 153 107 84
AflIII A'CryG_T
Cuts at: 0 120 1014 1422
Size: 120 894 408
Fragments arranged by size:
894 408 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1096 1422
Size: 1096 326
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1422
Size: 1048 32 342
Fragments arranged by size:
1048 342 32
AlwNI CAG_nnn'CTG
Cuts at: 0 417 1125 1377 1422
Size: 417 708 252 45
Fragments arranged by size:
708 417 252 45
ApaI G_GGCC'C
Cuts at: 0 1100 1238 1422
Size: 1100 138 184
Fragments arranged by size:
1100 184 138
ApoI r'AATT_y
Cuts at: 0 49 69 1422
Size: 49 20 1353
Fragments arranged by size:
1353 49 20
BamHI G'GATC_C
Cuts at: 0 942 1422
Size: 942 480
BanI G'GyrC_C
Cuts at: 0 1416 1422
Size: 1416 6
BanII G_rGCy'C
Cuts at: 0 346 409 1045 1100 1238 1422
Size: 346 63 636 55 138 184
Fragments arranged by size:
636 346 184 138 63 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 379 400 1422
Size: 337 42 21 1022
Fragments arranged by size:
1022 337 42 21
BbvCI CC'TCA_GC
Cuts at: 0 1345 1422
Size: 1345 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 720 727 754 1422
Size: 693 27 7 27 668
Fragments arranged by size:
693 668 27 27 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 43 1422
Size: 43 1379
BglI GCCn_nnn'nGGC
Cuts at: 0 1007 1422
Size: 1007 415
Bme1580I G_kGCm'C
Cuts at: 0 1100 1238 1421 1422
Size: 1100 138 183 1
Fragments arranged by size:
1100 183 138 1
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 901 1102 1422
Size: 144 757 201 320
Fragments arranged by size:
757 320 201 144
Bpu10I CC'TnA_GC
Cuts at: 0 1345 1422
Size: 1345 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1422
Size: 1081 341
BsaI GGTCTCn'nnnn_
Cuts at: 0 1270 1422
Size: 1270 152
BsaAI yAC'GTr
Cuts at: 0 1017 1026 1316 1422
Size: 1017 9 290 106
Fragments arranged by size:
1017 290 106 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 804 1336 1422
Size: 100 704 532 86
Fragments arranged by size:
704 532 100 86
BseYI C'CCAG_C
Cuts at: 0 778 1422
Size: 778 644
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1066 1422
Size: 533 533 356
Fragments arranged by size:
533 533 356
BsmI GAATG_Cn'
Cuts at: 0 43 533 1422
Size: 43 490 889
Fragments arranged by size:
889 490 43
Bsp1286I G_dGCh'C
Cuts at: 0 346 409 1045 1100 1238 1421 1422
Size: 346 63 636 55 138 183 1
Fragments arranged by size:
636 346 183 138 63 55 1
BspHI T'CATG_A
Cuts at: 0 1199 1422
Size: 1199 223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 736 843 1088 1422
Size: 736 107 245 334
Fragments arranged by size:
736 334 245 107
BsrFI r'CCGG_y
Cuts at: 0 863 1422
Size: 863 559
BssSI C'ACGA_G
Cuts at: 0 1048 1422
Size: 1048 374
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1377 1422
Size: 1377 45
BstEII G'GTnAC_C
Cuts at: 0 351 1422
Size: 351 1071
BstYI r'GATC_y
Cuts at: 0 280 942 1374 1422
Size: 280 662 432 48
Fragments arranged by size:
662 432 280 48
Bsu36I CC'TnA_GG
Cuts at: 0 347 1422
Size: 347 1075
BtgI C'CryG_G
Cuts at: 0 1165 1422
Size: 1165 257
BtsI GCAGTG_nn'
Cuts at: 0 709 1422
Size: 709 713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 426 461 1422
Size: 426 35 961
Fragments arranged by size:
961 426 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1253 1422
Size: 1253 169
EaeI y'GGCC_r
Cuts at: 0 716 1422
Size: 716 706
EarI CTCTTCn'nnn_
Cuts at: 0 232 334 1422
Size: 232 102 1088
Fragments arranged by size:
1088 232 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 275 439 523 781 901 1102 1162
Size: 144 131 164 84 258 120 201 60
Cuts at: 1162 1315 1422
Size: 153 107
Fragments arranged by size:
258 201 164 153 144 131 120 107
84 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1084 1422
Size: 1084 338
EcoO109I rG'GnC_Cy
Cuts at: 0 98 1056 1097 1234 1235 1246 1268 1422
Size: 98 958 41 137 1 11 22 154
Fragments arranged by size:
958 154 137 98 41 22 11 1
HaeII r_GCGC'y
Cuts at: 0 630 1422
Size: 630 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 1422
Size: 209 32 1181
Fragments arranged by size:
1181 209 32
HincII GTy'rAC
Cuts at: 0 681 1422
Size: 681 741
HindIII A'AGCT_T
Cuts at: 0 449 1361 1422
Size: 449 912 61
Fragments arranged by size:
912 449 61
MscI TGG'CCA
Cuts at: 0 718 1422
Size: 718 704
MslI CAynn'nnrTG
Cuts at: 0 896 914 1072 1422
Size: 896 18 158 350
Fragments arranged by size:
896 350 158 18
MspA1I CmG'CkG
Cuts at: 0 380 657 925 1422
Size: 380 277 268 497
Fragments arranged by size:
497 380 277 268
NaeI GCC'GGC
Cuts at: 0 865 1422
Size: 865 557
NcoI C'CATG_G
Cuts at: 0 1165 1422
Size: 1165 257
NdeI CA'TA_TG
Cuts at: 0 1392 1422
Size: 1392 30
NgoMIV G'CCGG_C
Cuts at: 0 863 1422
Size: 863 559
NspI r_CATG'y
Cuts at: 0 94 124 829 1140 1422
Size: 94 30 705 311 282
Fragments arranged by size:
705 311 282 94 30
PciI A'CATG_T
Cuts at: 0 120 1422
Size: 120 1302
PflMI CCAn_nnn'nTGG
Cuts at: 0 22 1125 1422
Size: 22 1103 297
Fragments arranged by size:
1103 297 22
PfoI T'CCnGG_A
Cuts at: 0 292 1422
Size: 292 1130
PmlI CAC'GTG
Cuts at: 0 1017 1422
Size: 1017 405
PpuMI rG'GwC_Cy
Cuts at: 0 98 1056 1246 1422
Size: 98 958 190 176
Fragments arranged by size:
958 190 176 98
PshAI GACnn'nnGTC
Cuts at: 0 109 956 1422
Size: 109 847 466
Fragments arranged by size:
847 466 109
PspOMI G'GGCC_C
Cuts at: 0 1096 1234 1422
Size: 1096 138 188
Fragments arranged by size:
1096 188 138
PstI C_TGCA'G
Cuts at: 0 854 1286 1373 1422
Size: 854 432 87 49
Fragments arranged by size:
854 432 87 49
PvuII CAG'CTG
Cuts at: 0 380 925 1422
Size: 380 545 497
Fragments arranged by size:
545 497 380
SanDI GG'GwC_CC
Cuts at: 0 98 1246 1422
Size: 98 1148 176
Fragments arranged by size:
1148 176 98
SapI GCTCTTCn'nnn_
Cuts at: 0 334 1422
Size: 334 1088
SfcI C'TryA_G
Cuts at: 0 80 381 850 1282 1369 1422
Size: 80 301 469 432 87 53
Fragments arranged by size:
469 432 301 87 80 53
SmlI C'TyrA_G
Cuts at: 0 1060 1422
Size: 1060 362
SphI G_CATG'C
Cuts at: 0 1140 1422
Size: 1140 282
StyI C'CwwG_G
Cuts at: 0 135 617 1165 1422
Size: 135 482 548 257
Fragments arranged by size:
548 482 257 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 567 1422
Size: 567 855
TatI w'GTAC_w
Cuts at: 0 698 1422
Size: 698 724
XmnI GAAnn'nnTTC
Cuts at: 0 508 649 1422
Size: 508 141 773
Fragments arranged by size:
773 508 141
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BglI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsgI BsmI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SanDI SapI SfcI SmlI SphI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstBI BstXI
BstZ17I BtgZI ClaI DraI DraIII EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MmeI NarI NheI NotI NruI
NsiI PacI PasI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI