Restriction map for
pF1KE0948
(Linear) (Six-base) MAPSORT of: KIEE0948.seq Check: 1251 from: 1 to: 1278
KIEE0948.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 101 1278
Size: 101 1177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 814 1152 1278
Size: 394 420 338 126
Fragments arranged by size:
420 394 338 126
AfeI AGC'GCT
Cuts at: 0 865 1278
Size: 865 413
AhdI GACnn_n'nnGTC
Cuts at: 0 594 1278
Size: 594 684
AleI CACnn'nnGTG
Cuts at: 0 523 1278
Size: 523 755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 392 424 1278
Size: 392 32 854
Fragments arranged by size:
854 392 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1071 1278
Size: 1071 207
ApaI G_GGCC'C
Cuts at: 0 949 1278
Size: 949 329
ApaLI G'TGCA_C
Cuts at: 0 1216 1278
Size: 1216 62
AvaI C'yCGr_G
Cuts at: 0 35 1278
Size: 35 1243
BanI G'GyrC_C
Cuts at: 0 529 546 1278
Size: 529 17 732
Fragments arranged by size:
732 529 17
BanII G_rGCy'C
Cuts at: 0 56 88 791 848 949 1043 1106 1278
Size: 56 32 703 57 101 94 63 172
Fragments arranged by size:
703 172 101 94 63 57 56 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 832 1086 1278
Size: 832 254 192
Fragments arranged by size:
832 254 192
BlpI GC'TnA_GC
Cuts at: 0 303 1278
Size: 303 975
Bme1580I G_kGCm'C
Cuts at: 0 532 949 1220 1278
Size: 532 417 271 58
Fragments arranged by size:
532 417 271 58
BmgBI CAC'GTC
Cuts at: 0 459 1278
Size: 459 819
BmrI ACTGGGnnnn_n'
Cuts at: 0 1059 1278
Size: 1059 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 1278
Size: 915 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 355 724 1168 1177 1278
Size: 178 177 369 444 9 101
Fragments arranged by size:
444 369 178 177 101 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 1125 1155 1278
Size: 232 30 863 30 123
Fragments arranged by size:
863 232 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 175 352 904 1165 1195 1278
Size: 64 111 177 552 261 30 83
Fragments arranged by size:
552 261 177 111 83 64 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1000 1255 1278
Size: 376 624 255 23
Fragments arranged by size:
624 376 255 23
BsiEI CG_ry'CG
Cuts at: 0 72 855 979 1278
Size: 72 783 124 299
Fragments arranged by size:
783 299 124 72
BsiHKAI G_wGCw'C
Cuts at: 0 56 265 791 848 1106 1220 1278
Size: 56 209 526 57 258 114 58
Fragments arranged by size:
526 258 209 114 58 57 56
Bsp1286I G_dGCh'C
Cuts at: 0 56 88 265 532 791 848 949 1043
Size: 56 32 177 267 259 57 101 94
Cuts at: 1043 1106 1220 1278
Size: 63 114 58
Fragments arranged by size:
267 259 177 114 101 94 63 58
57 56 32
BsrBI CCG'CTC
Cuts at: 0 1127 1278
Size: 1127 151
BsrDI GCAATG_nn'
Cuts at: 0 347 1278
Size: 347 931
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 1278
Size: 621 657
BstYI r'GATC_y
Cuts at: 0 407 1278
Size: 407 871
Bsu36I CC'TnA_GG
Cuts at: 0 47 551 1278
Size: 47 504 727
Fragments arranged by size:
727 504 47
BtgI C'CryG_G
Cuts at: 0 776 1278
Size: 776 502
ClaI AT'CG_AT
Cuts at: 0 113 1278
Size: 113 1165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 113 148 1278
Size: 113 35 1130
Fragments arranged by size:
1130 113 35
DraIII CAC_nnn'GTG
Cuts at: 0 1224 1278
Size: 1224 54
EaeI y'GGCC_r
Cuts at: 0 137 623 779 976 1175 1278
Size: 137 486 156 197 199 103
Fragments arranged by size:
486 199 197 156 137 103
EagI C'GGCC_G
Cuts at: 0 976 1278
Size: 976 302
EarI CTCTTCn'nnn_
Cuts at: 0 61 1278
Size: 61 1217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 943 1278
Size: 484 459 335
Fragments arranged by size:
484 459 335
EcoICRI GAG'CTC
Cuts at: 0 54 789 846 1104 1278
Size: 54 735 57 258 174
Fragments arranged by size:
735 258 174 57 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 355 394 724 814 1152 1168 1177
Size: 178 177 39 330 90 338 16 9
Cuts at: 1177 1278
Size: 101
Fragments arranged by size:
338 330 178 177 101 90 39 16
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 1177 1278
Size: 160 1017 101
Fragments arranged by size:
1017 160 101
EcoO109I rG'GnC_Cy
Cuts at: 0 328 378 945 946 1061 1278
Size: 328 50 567 1 115 217
Fragments arranged by size:
567 328 217 115 50 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 849 1278
Size: 849 429
HaeII r_GCGC'y
Cuts at: 0 867 1278
Size: 867 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1278
Size: 580 32 666
Fragments arranged by size:
666 580 32
HindIII A'AGCT_T
Cuts at: 0 994 1278
Size: 994 284
MscI TGG'CCA
Cuts at: 0 139 625 1278
Size: 139 486 653
Fragments arranged by size:
653 486 139
MslI CAynn'nnrTG
Cuts at: 0 523 1278
Size: 523 755
PasI CC'CwG_GG
Cuts at: 0 600 1278
Size: 600 678
PflMI CCAn_nnn'nTGG
Cuts at: 0 272 1278
Size: 272 1006
PpuMI rG'GwC_Cy
Cuts at: 0 328 1061 1278
Size: 328 733 217
Fragments arranged by size:
733 328 217
PspOMI G'GGCC_C
Cuts at: 0 945 1278
Size: 945 333
SacI G_AGCT'C
Cuts at: 0 56 791 848 1106 1278
Size: 56 735 57 258 172
Fragments arranged by size:
735 258 172 57 56
SapI GCTCTTCn'nnn_
Cuts at: 0 61 1278
Size: 61 1217
SexAI A'CCwGG_T
Cuts at: 0 324 1220 1278
Size: 324 896 58
Fragments arranged by size:
896 324 58
SmlI C'TyrA_G
Cuts at: 0 35 1278
Size: 35 1243
StuI AGG'CCT
Cuts at: 0 745 1278
Size: 745 533
StyI C'CwwG_G
Cuts at: 0 626 782 1278
Size: 626 156 496
Fragments arranged by size:
626 496 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 711 1129 1278
Size: 711 418 149
Fragments arranged by size:
711 418 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1125 1157 1252 1278
Size: 1125 32 95 26
Fragments arranged by size:
1125 95 32 26
XhoI C'TCGA_G
Cuts at: 0 35 1278
Size: 35 1243
XmnI GAAnn'nnTTC
Cuts at: 0 520 1165 1278
Size: 520 645 113
Fragments arranged by size:
645 520 113
Enzymes that do cut:
AccI AcuI AfeI AhdI AleI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaXI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrDI BstXI BstYI Bsu36I BtgI ClaI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HindIII MscI MslI
PasI PflMI PpuMI PspOMI SacI SapI SexAI SmlI
StuI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI
DraI DrdI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XcmI XmaI ZraI