Restriction map for
pF1KE0946
(Linear) (Six-base) MAPSORT of: KIEE0946.seq Check: 5152 from: 1 to: 1101
KIEE0946.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AatII G_ACGT'C
Cuts at: 0 395 770 1101
Size: 395 375 331
Fragments arranged by size:
395 375 331
AccI GT'mk_AC
Cuts at: 0 257 1045 1101
Size: 257 788 56
Fragments arranged by size:
788 257 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 447 1101
Size: 121 326 654
Fragments arranged by size:
654 326 121
AfeI AGC'GCT
Cuts at: 0 478 1101
Size: 478 623
AhdI GACnn_n'nnGTC
Cuts at: 0 918 1101
Size: 918 183
AlwNI CAG_nnn'CTG
Cuts at: 0 806 1101
Size: 806 295
ApaI G_GGCC'C
Cuts at: 0 1070 1098 1101
Size: 1070 28 3
Fragments arranged by size:
1070 28 3
ApoI r'AATT_y
Cuts at: 0 48 163 1101
Size: 48 115 938
Fragments arranged by size:
938 115 48
AvaI C'yCGr_G
Cuts at: 0 173 295 311 872 982 1101
Size: 173 122 16 561 110 119
Fragments arranged by size:
561 173 122 119 110 16
AvrII C'CTAG_G
Cuts at: 0 603 1101
Size: 603 498
BanII G_rGCy'C
Cuts at: 0 64 275 321 1070 1098 1101
Size: 64 211 46 749 28 3
Fragments arranged by size:
749 211 64 46 28 3
BbvCI CC'TCA_GC
Cuts at: 0 799 1101
Size: 799 302
BciVI GTATCCnnnnn_n'
Cuts at: 0 688 1101
Size: 688 413
BglII A'GATC_T
Cuts at: 0 230 1101
Size: 230 871
Bme1580I G_kGCm'C
Cuts at: 0 1070 1098 1101
Size: 1070 28 3
Fragments arranged by size:
1070 28 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 1101
Size: 52 1049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 955 1067 1101
Size: 618 337 112 34
Fragments arranged by size:
618 337 112 34
Bpu10I CC'TnA_GC
Cuts at: 0 799 1101
Size: 799 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 394 1101
Size: 4 390 707
Fragments arranged by size:
707 390 4
BsaBI GATnn'nnATC
Cuts at: 0 121 1101
Size: 121 980
BsaHI Gr'CG_yC
Cuts at: 0 392 767 1101
Size: 392 375 334
Fragments arranged by size:
392 375 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 637 667 1101
Size: 193 30 414 30 434
Fragments arranged by size:
434 414 193 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1101
Size: 283 818
BseYI C'CCAG_C
Cuts at: 0 1033 1101
Size: 1033 68
BsiEI CG_ry'CG
Cuts at: 0 132 1101
Size: 132 969
BsiHKAI G_wGCw'C
Cuts at: 0 275 692 1101
Size: 275 417 409
Fragments arranged by size:
417 409 275
BsmI GAATG_Cn'
Cuts at: 0 566 1101
Size: 566 535
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 1101
Size: 1081 20
Bsp1286I G_dGCh'C
Cuts at: 0 64 275 321 692 1070 1098 1101
Size: 64 211 46 371 378 28 3
Fragments arranged by size:
378 371 211 64 46 28 3
BsrDI GCAATG_nn'
Cuts at: 0 1001 1101
Size: 1001 100
BsrFI r'CCGG_y
Cuts at: 0 773 1101
Size: 773 328
BstEII G'GTnAC_C
Cuts at: 0 530 1101
Size: 530 571
BstXI CCAn_nnnn'nTGG
Cuts at: 0 933 1101
Size: 933 168
BstYI r'GATC_y
Cuts at: 0 230 794 832 1019 1101
Size: 230 564 38 187 82
Fragments arranged by size:
564 230 187 82 38
Bsu36I CC'TnA_GG
Cuts at: 0 941 1101
Size: 941 160
BtsI GCAGTG_nn'
Cuts at: 0 204 804 1101
Size: 204 600 297
Fragments arranged by size:
600 297 204
DrdI GACnn_nn'nnGTC
Cuts at: 0 13 391 1101
Size: 13 378 710
Fragments arranged by size:
710 378 13
EaeI y'GGCC_r
Cuts at: 0 191 365 845 1101
Size: 191 174 480 256
Fragments arranged by size:
480 256 191 174
EcoICRI GAG'CTC
Cuts at: 0 273 1101
Size: 273 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 447 618 955 1067 1101
Size: 121 326 171 337 112 34
Fragments arranged by size:
337 326 171 121 112 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 433 1101
Size: 325 108 668
Fragments arranged by size:
668 325 108
EcoO109I rG'GnC_Cy
Cuts at: 0 141 405 738 1066 1067 1101
Size: 141 264 333 328 1 34
Fragments arranged by size:
333 328 264 141 34 1
HaeII r_GCGC'y
Cuts at: 0 99 480 1101
Size: 99 381 621
Fragments arranged by size:
621 381 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1101
Size: 904 32 165
Fragments arranged by size:
904 165 32
HincII GTy'rAC
Cuts at: 0 699 771 1046 1101
Size: 699 72 275 55
Fragments arranged by size:
699 275 72 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 680 995 1101
Size: 482 198 315 106
Fragments arranged by size:
482 315 198 106
MscI TGG'CCA
Cuts at: 0 193 367 847 1101
Size: 193 174 480 254
Fragments arranged by size:
480 254 193 174
MslI CAynn'nnrTG
Cuts at: 0 265 1101
Size: 265 836
NspI r_CATG'y
Cuts at: 0 756 1101
Size: 756 345
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1101
Size: 387 714
PpuMI rG'GwC_Cy
Cuts at: 0 405 738 1101
Size: 405 333 363
Fragments arranged by size:
405 363 333
PshAI GACnn'nnGTC
Cuts at: 0 497 1101
Size: 497 604
PspOMI G'GGCC_C
Cuts at: 0 1066 1094 1101
Size: 1066 28 7
Fragments arranged by size:
1066 28 7
PspXI vC'TCGA_Gb
Cuts at: 0 295 1101
Size: 295 806
PstI C_TGCA'G
Cuts at: 0 563 761 1101
Size: 563 198 340
Fragments arranged by size:
563 340 198
SacI G_AGCT'C
Cuts at: 0 275 1101
Size: 275 826
SalI G'TCGA_C
Cuts at: 0 1044 1101
Size: 1044 57
ScaI AGT'ACT
Cuts at: 0 376 1101
Size: 376 725
SfcI C'TryA_G
Cuts at: 0 559 757 1101
Size: 559 198 344
Fragments arranged by size:
559 344 198
SmaI CCC'GGG
Cuts at: 0 175 313 874 1101
Size: 175 138 561 227
Fragments arranged by size:
561 227 175 138
SmlI C'TyrA_G
Cuts at: 0 19 295 409 1101
Size: 19 276 114 692
Fragments arranged by size:
692 276 114 19
StyI C'CwwG_G
Cuts at: 0 362 581 603 821 1101
Size: 362 219 22 218 280
Fragments arranged by size:
362 280 219 218 22
TatI w'GTAC_w
Cuts at: 0 374 1101
Size: 374 727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 397 429 1101
Size: 343 32 22 32 672
Fragments arranged by size:
672 343 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 394 1101
Size: 394 707
XhoI C'TCGA_G
Cuts at: 0 295 1101
Size: 295 806
XmaI C'CCGG_G
Cuts at: 0 173 311 872 1101
Size: 173 138 561 229
Fragments arranged by size:
561 229 173 138
ZraI GAC'GTC
Cuts at: 0 393 768 1101
Size: 393 375 333
Fragments arranged by size:
393 375 333
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AlwNI ApaI ApoI
AvaI AvrII BanII BbvCI BciVI BglII Bme1580I BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI BstEII
BstXI BstYI Bsu36I BtsI DrdI EaeI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI MslI
NspI PflMI PpuMI PshAI PspOMI PspXI PstI SacI
SalI ScaI SfcI SmaI SmlI StyI TatI TstI
Tth111I XhoI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbsI BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BsaI BsaAI BsaWI BsgI BsiWI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EagI
EarI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PsiI
PsrI PvuI PvuII RsrII SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII XbaI XcmI XmnI