Restriction map for
pF1KE0945
(Linear) (Six-base) MAPSORT of: KIEE0945.seq Check: 8372 from: 1 to: 1050
KIEE0945.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 271 645 1050
Size: 162 109 374 405
Fragments arranged by size:
405 374 162 109
AflIII A'CryG_T
Cuts at: 0 396 518 1050
Size: 396 122 532
Fragments arranged by size:
532 396 122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 329 1050
Size: 329 721
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 694 726 1050
Size: 694 32 324
Fragments arranged by size:
694 324 32
AlwNI CAG_nnn'CTG
Cuts at: 0 375 645 1050
Size: 375 270 405
Fragments arranged by size:
405 375 270
AseI AT'TA_AT
Cuts at: 0 566 1050
Size: 566 484
AvaI C'yCGr_G
Cuts at: 0 41 1050
Size: 41 1009
BanI G'GyrC_C
Cuts at: 0 48 1050
Size: 48 1002
BbsI GAAGACnn'nnnn_
Cuts at: 0 634 1050
Size: 634 416
BciVI GTATCCnnnnn_n'
Cuts at: 0 999 1050
Size: 999 51
BclI T'GATC_A
Cuts at: 0 74 1050
Size: 74 976
BglI GCCn_nnn'nGGC
Cuts at: 0 590 1050
Size: 590 460
Bme1580I G_kGCm'C
Cuts at: 0 595 884 1050
Size: 595 289 166
Fragments arranged by size:
595 289 166
BmgBI CAC'GTC
Cuts at: 0 795 1050
Size: 795 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 633 1050
Size: 633 417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 965 1050
Size: 965 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 556 749 849 1003 1050
Size: 358 198 193 100 154 47
Fragments arranged by size:
358 198 193 154 100 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1050
Size: 489 561
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1050
Size: 979 71
BsaAI yAC'GTr
Cuts at: 0 521 1050
Size: 521 529
BsaHI Gr'CG_yC
Cuts at: 0 686 1050
Size: 686 364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 779 809 1050
Size: 694 30 55 30 241
Fragments arranged by size:
694 241 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 238 1050
Size: 122 116 812
Fragments arranged by size:
812 122 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1050
Size: 559 491
BsiHKAI G_wGCw'C
Cuts at: 0 308 1050
Size: 308 742
Bsp1286I G_dGCh'C
Cuts at: 0 308 595 884 1050
Size: 308 287 289 166
Fragments arranged by size:
308 289 287 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 629 1050
Size: 581 48 421
Fragments arranged by size:
581 421 48
BstYI r'GATC_y
Cuts at: 0 851 1050
Size: 851 199
BtgI C'CryG_G
Cuts at: 0 360 545 1050
Size: 360 185 505
Fragments arranged by size:
505 360 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 111 1050
Size: 111 939
BtsI GCAGTG_nn'
Cuts at: 0 965 1050
Size: 965 85
EaeI y'GGCC_r
Cuts at: 0 542 732 1050
Size: 542 190 318
Fragments arranged by size:
542 318 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 271 358 556 645 749 849 1003
Size: 162 109 87 198 89 104 100 154
Cuts at: 1003 1050
Size: 47
Fragments arranged by size:
198 162 154 109 104 100 89 87
47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 300 1050
Size: 300 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 1050
Size: 78 32 940
Fragments arranged by size:
940 78 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1050
Size: 638 412
MscI TGG'CCA
Cuts at: 0 544 1050
Size: 544 506
MspA1I CmG'CkG
Cuts at: 0 58 249 1050
Size: 58 191 801
Fragments arranged by size:
801 191 58
NcoI C'CATG_G
Cuts at: 0 360 545 1050
Size: 360 185 505
Fragments arranged by size:
505 360 185
NdeI CA'TA_TG
Cuts at: 0 825 1050
Size: 825 225
NspI r_CATG'y
Cuts at: 0 400 938 1050
Size: 400 538 112
Fragments arranged by size:
538 400 112
PciI A'CATG_T
Cuts at: 0 396 1050
Size: 396 654
PflMI CCAn_nnn'nTGG
Cuts at: 0 61 1050
Size: 61 989
PfoI T'CCnGG_A
Cuts at: 0 712 1050
Size: 712 338
PmlI CAC'GTG
Cuts at: 0 521 1050
Size: 521 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1050
Size: 694 32 324
Fragments arranged by size:
694 324 32
PstI C_TGCA'G
Cuts at: 0 371 1050
Size: 371 679
PvuII CAG'CTG
Cuts at: 0 58 249 1050
Size: 58 191 801
Fragments arranged by size:
801 191 58
SfcI C'TryA_G
Cuts at: 0 367 1050
Size: 367 683
SmlI C'TyrA_G
Cuts at: 0 468 1050
Size: 468 582
SphI G_CATG'C
Cuts at: 0 938 1050
Size: 938 112
StuI AGG'CCT
Cuts at: 0 32 1050
Size: 32 1018
StyI C'CwwG_G
Cuts at: 0 208 360 387 545 1050
Size: 208 152 27 158 505
Fragments arranged by size:
505 208 158 152 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 147 179 779 811 1050
Size: 147 32 600 32 239
Fragments arranged by size:
600 239 147 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 553 633 1050
Size: 553 80 417
Fragments arranged by size:
553 417 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 773 873 1050
Size: 773 100 177
Fragments arranged by size:
773 177 100
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI AseI AvaI BanI
BbsI BciVI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BstYI BtgI BtgZI BtsI EaeI
Eco57MI FalI Hin4I MmeI MscI MspA1I NcoI NdeI
NspI PciI PflMI PfoI PmlI PpiI PstI PvuII
SfcI SmlI SphI StuI StyI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaI ApaLI ApoI AscI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BfrBI BglII
BlpI BmtI Bpu10I BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI TaqII TatI XbaI
XhoI XmaI XmnI ZraI