Restriction map for
pF1KE0944
(Linear) (Six-base) MAPSORT of: KIEE0944.seq Check: 5144 from: 1 to: 1029
KIEE0944.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 436 1029
Size: 436 593
AatII G_ACGT'C
Cuts at: 0 995 1029
Size: 995 34
AccI GT'mk_AC
Cuts at: 0 16 1029
Size: 16 1013
Acc65I G'GTAC_C
Cuts at: 0 752 1029
Size: 752 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1029
Size: 771 258
AflIII A'CryG_T
Cuts at: 0 707 1029
Size: 707 322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 1029
Size: 115 32 882
Fragments arranged by size:
882 115 32
ApoI r'AATT_y
Cuts at: 0 538 902 945 1029
Size: 538 364 43 84
Fragments arranged by size:
538 364 84 43
AvaI C'yCGr_G
Cuts at: 0 135 145 566 690 1029
Size: 135 10 421 124 339
Fragments arranged by size:
421 339 135 124 10
BanI G'GyrC_C
Cuts at: 0 303 752 984 1029
Size: 303 449 232 45
Fragments arranged by size:
449 303 232 45
BanII G_rGCy'C
Cuts at: 0 104 142 878 1029
Size: 104 38 736 151
Fragments arranged by size:
736 151 104 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 1029
Size: 596 433
BfrBI ATG'CAT
Cuts at: 0 411 1029
Size: 411 618
BglII A'GATC_T
Cuts at: 0 665 1029
Size: 665 364
BmgBI CAC'GTC
Cuts at: 0 708 1029
Size: 708 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1029
Size: 229 800
Bpu10I CC'TnA_GC
Cuts at: 0 962 1029
Size: 962 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1029
Size: 511 518
BsaI GGTCTCn'nnnn_
Cuts at: 0 209 361 1029
Size: 209 152 668
Fragments arranged by size:
668 209 152
BsaAI yAC'GTr
Cuts at: 0 654 1029
Size: 654 375
BsaHI Gr'CG_yC
Cuts at: 0 593 992 1029
Size: 593 399 37
Fragments arranged by size:
593 399 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 206 236 247 277 1029
Size: 115 30 61 30 11 30 752
Fragments arranged by size:
752 115 61 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 460 463 1029
Size: 268 192 3 566
Fragments arranged by size:
566 268 192 3
BseYI C'CCAG_C
Cuts at: 0 103 775 1029
Size: 103 672 254
Fragments arranged by size:
672 254 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1029
Size: 226 803
BsmI GAATG_Cn'
Cuts at: 0 411 860 1029
Size: 411 449 169
Fragments arranged by size:
449 411 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 945 1029
Size: 945 84
Bsp1286I G_dGCh'C
Cuts at: 0 104 142 878 1029
Size: 104 38 736 151
Fragments arranged by size:
736 151 104 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 436 1029
Size: 436 593
BsrBI CCG'CTC
Cuts at: 0 885 1029
Size: 885 144
BsrDI GCAATG_nn'
Cuts at: 0 165 1029
Size: 165 864
BsrGI T'GTAC_A
Cuts at: 0 731 1029
Size: 731 298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 958 1029
Size: 958 71
BstBI TT'CG_AA
Cuts at: 0 71 1029
Size: 71 958
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1029
Size: 744 285
BstYI r'GATC_y
Cuts at: 0 665 794 1029
Size: 665 129 235
Fragments arranged by size:
665 235 129
BtgI C'CryG_G
Cuts at: 0 95 1029
Size: 95 934
BtsI GCAGTG_nn'
Cuts at: 0 491 492 1029
Size: 491 1 537
Fragments arranged by size:
537 491 1
EaeI y'GGCC_r
Cuts at: 0 804 1029
Size: 804 225
EarI CTCTTCn'nnn_
Cuts at: 0 68 551 790 998 1029
Size: 68 483 239 208 31
Fragments arranged by size:
483 239 208 68 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 771 1029
Size: 229 542 258
Fragments arranged by size:
542 258 229
EcoO109I rG'GnC_Cy
Cuts at: 0 522 1029
Size: 522 507
EcoRI G'AATT_C
Cuts at: 0 945 1029
Size: 945 84
EcoRV GAT'ATC
Cuts at: 0 279 1029
Size: 279 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 610 642 814 846 1029
Size: 67 32 511 32 172 32 183
Fragments arranged by size:
511 183 172 67 32 32 32
HincII GTy'rAC
Cuts at: 0 996 1029
Size: 996 33
KpnI G_GTAC'C
Cuts at: 0 756 1029
Size: 756 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 653 1029
Size: 179 474 376
Fragments arranged by size:
474 376 179
MscI TGG'CCA
Cuts at: 0 806 1029
Size: 806 223
MslI CAynn'nnrTG
Cuts at: 0 497 742 1029
Size: 497 245 287
Fragments arranged by size:
497 287 245
MspA1I CmG'CkG
Cuts at: 0 97 248 284 981 1029
Size: 97 151 36 697 48
Fragments arranged by size:
697 151 97 48 36
NdeI CA'TA_TG
Cuts at: 0 954 1029
Size: 954 75
NsiI A_TGCA'T
Cuts at: 0 413 1029
Size: 413 616
NspI r_CATG'y
Cuts at: 0 411 1029
Size: 411 618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 1029
Size: 115 32 882
Fragments arranged by size:
882 115 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 1029
Size: 522 507
PshAI GACnn'nnGTC
Cuts at: 0 568 1029
Size: 568 461
PvuII CAG'CTG
Cuts at: 0 284 981 1029
Size: 284 697 48
Fragments arranged by size:
697 284 48
SacII CC_GC'GG
Cuts at: 0 98 1029
Size: 98 931
SfcI C'TryA_G
Cuts at: 0 926 1029
Size: 926 103
SmlI C'TyrA_G
Cuts at: 0 526 1029
Size: 526 503
SnaBI TAC'GTA
Cuts at: 0 654 1029
Size: 654 375
StyI C'CwwG_G
Cuts at: 0 836 916 1029
Size: 836 80 113
Fragments arranged by size:
836 113 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1029
Size: 352 677
TatI w'GTAC_w
Cuts at: 0 731 1029
Size: 731 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 1029
Size: 247 32 750
Fragments arranged by size:
750 247 32
Tth111I GACn'n_nGTC
Cuts at: 0 828 1029
Size: 828 201
ZraI GAC'GTC
Cuts at: 0 993 1029
Size: 993 36
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AloI ApoI
AvaI BanI BanII BbsI BfrBI BglII BmgBI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BstBI BstXI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII KpnI MmeI
MscI MslI MspA1I NdeI NsiI NspI PpiI PpuMI
PshAI PvuII SacII SfcI SmlI SnaBI StyI TaqII
TatI TstI Tth111I ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AlfI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmrI BmtI BplI BsaBI BsaWI BsiEI BsiHKAI BsiWI
BspEI BspHI BsrFI BssHII BssSI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MluI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI