Restriction map for
pF1KE0943
(Linear) (Six-base) MAPSORT of: KIEE0943.seq Check: 7160 from: 1 to: 1008
KIEE0943.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 182 545 750 813 1008
Size: 182 363 205 63 195
Fragments arranged by size:
363 205 195 182 63
AatII G_ACGT'C
Cuts at: 0 935 1008
Size: 935 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1008
Size: 816 192
AleI CACnn'nnGTG
Cuts at: 0 733 1008
Size: 733 275
AlwNI CAG_nnn'CTG
Cuts at: 0 303 379 1008
Size: 303 76 629
Fragments arranged by size:
629 303 76
ApaI G_GGCC'C
Cuts at: 0 494 612 1008
Size: 494 118 396
Fragments arranged by size:
494 396 118
ApaLI G'TGCA_C
Cuts at: 0 127 1008
Size: 127 881
AvaI C'yCGr_G
Cuts at: 0 351 493 1008
Size: 351 142 515
Fragments arranged by size:
515 351 142
BanI G'GyrC_C
Cuts at: 0 19 683 913 1008
Size: 19 664 230 95
Fragments arranged by size:
664 230 95 19
BanII G_rGCy'C
Cuts at: 0 409 494 612 1008
Size: 409 85 118 396
Fragments arranged by size:
409 396 118 85
BglI GCCn_nnn'nGGC
Cuts at: 0 440 1008
Size: 440 568
Bme1580I G_kGCm'C
Cuts at: 0 131 494 612 1008
Size: 131 363 118 396
Fragments arranged by size:
396 363 131 118
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 1008
Size: 306 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1008
Size: 194 814
BsaHI Gr'CG_yC
Cuts at: 0 932 1008
Size: 932 76
BsaWI w'CCGG_w
Cuts at: 0 284 964 1008
Size: 284 680 44
Fragments arranged by size:
680 284 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 889 919 1008
Size: 367 30 492 30 89
Fragments arranged by size:
492 367 89 30 30
BseYI C'CCAG_C
Cuts at: 0 160 1008
Size: 160 848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1008
Size: 841 167
BsiEI CG_ry'CG
Cuts at: 0 152 1008
Size: 152 856
BsiHKAI G_wGCw'C
Cuts at: 0 131 409 788 1008
Size: 131 278 379 220
Fragments arranged by size:
379 278 220 131
Bsp1286I G_dGCh'C
Cuts at: 0 131 409 494 612 788 1008
Size: 131 278 85 118 176 220
Fragments arranged by size:
278 220 176 131 118 85
BspEI T'CCGG_A
Cuts at: 0 284 1008
Size: 284 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 545 750 813 1008
Size: 182 363 205 63 195
Fragments arranged by size:
363 205 195 182 63
BsrBI CCG'CTC
Cuts at: 0 94 156 1008
Size: 94 62 852
Fragments arranged by size:
852 94 62
BsrDI GCAATG_nn'
Cuts at: 0 816 1008
Size: 816 192
BsrFI r'CCGG_y
Cuts at: 0 215 222 317 503 1008
Size: 215 7 95 186 505
Fragments arranged by size:
505 215 186 95 7
BsrGI T'GTAC_A
Cuts at: 0 2 1008
Size: 2 1006
BssHII G'CGCG_C
Cuts at: 0 112 1008
Size: 112 896
BstAPI GCAn_nnn'nTGC
Cuts at: 0 792 1008
Size: 792 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 621 1008
Size: 453 168 387
Fragments arranged by size:
453 387 168
BstYI r'GATC_y
Cuts at: 0 278 1008
Size: 278 730
BtgI C'CryG_G
Cuts at: 0 386 945 1008
Size: 386 559 63
Fragments arranged by size:
559 386 63
DraIII CAC_nnn'GTG
Cuts at: 0 314 1008
Size: 314 694
EaeI y'GGCC_r
Cuts at: 0 149 319 485 508 566 1008
Size: 149 170 166 23 58 442
Fragments arranged by size:
442 170 166 149 58 23
EagI C'GGCC_G
Cuts at: 0 149 1008
Size: 149 859
EcoICRI GAG'CTC
Cuts at: 0 407 1008
Size: 407 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 816 1008
Size: 194 622 192
Fragments arranged by size:
622 194 192
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 1008
Size: 178 830
EcoO109I rG'GnC_Cy
Cuts at: 0 442 467 559 576 609 719 905 1008
Size: 442 25 92 17 33 110 186 103
Fragments arranged by size:
442 186 110 103 92 33 25 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 740 772 889 921 1008
Size: 367 32 341 32 117 32 87
Fragments arranged by size:
367 341 117 87 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1008
Size: 29 979
MslI CAynn'nnrTG
Cuts at: 0 689 733 1008
Size: 689 44 275
Fragments arranged by size:
689 275 44
MspA1I CmG'CkG
Cuts at: 0 300 543 1008
Size: 300 243 465
Fragments arranged by size:
465 300 243
NaeI GCC'GGC
Cuts at: 0 224 319 505 1008
Size: 224 95 186 503
Fragments arranged by size:
503 224 186 95
NcoI C'CATG_G
Cuts at: 0 386 1008
Size: 386 622
NgoMIV G'CCGG_C
Cuts at: 0 222 317 503 1008
Size: 222 95 186 505
Fragments arranged by size:
505 222 186 95
NspI r_CATG'y
Cuts at: 0 996 1008
Size: 996 12
PasI CC'CwG_GG
Cuts at: 0 582 1008
Size: 582 426
PflMI CCAn_nnn'nTGG
Cuts at: 0 392 605 660 745 1008
Size: 392 213 55 85 263
Fragments arranged by size:
392 263 213 85 55
PfoI T'CCnGG_A
Cuts at: 0 357 1008
Size: 357 651
PpuMI rG'GwC_Cy
Cuts at: 0 467 576 719 905 1008
Size: 467 109 143 186 103
Fragments arranged by size:
467 186 143 109 103
PspOMI G'GGCC_C
Cuts at: 0 490 608 1008
Size: 490 118 400
Fragments arranged by size:
490 400 118
PstI C_TGCA'G
Cuts at: 0 182 476 542 1001 1008
Size: 182 294 66 459 7
Fragments arranged by size:
459 294 182 66 7
PvuII CAG'CTG
Cuts at: 0 543 1008
Size: 543 465
SacI G_AGCT'C
Cuts at: 0 409 1008
Size: 409 599
SanDI GG'GwC_CC
Cuts at: 0 467 719 905 1008
Size: 467 252 186 103
Fragments arranged by size:
467 252 186 103
SexAI A'CCwGG_T
Cuts at: 0 452 1008
Size: 452 556
SfcI C'TryA_G
Cuts at: 0 178 472 538 997 1008
Size: 178 294 66 459 11
Fragments arranged by size:
459 294 178 66 11
SphI G_CATG'C
Cuts at: 0 996 1008
Size: 996 12
StuI AGG'CCT
Cuts at: 0 349 1008
Size: 349 659
StyI C'CwwG_G
Cuts at: 0 386 1008
Size: 386 622
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 325 609 701 1008
Size: 156 169 284 92 307
Fragments arranged by size:
307 284 169 156 92
TatI w'GTAC_w
Cuts at: 0 2 1008
Size: 2 1006
ZraI GAC'GTC
Cuts at: 0 933 1008
Size: 933 75
Enzymes that do cut:
AarI AatII AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BglI Bme1580I BmrI BpmI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI
BtgI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I MmeI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SanDI SexAI SfcI SphI StuI StyI TaqII TatI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BseRI BsiWI BsmI BsmBI BspHI BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI