Restriction map for
pF1KE0942
(Linear) (Six-base) MAPSORT of: KIEE0942.seq Check: 6817 from: 1 to: 882
KIEE0942.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AflIII A'CryG_T
Cuts at: 0 300 882
Size: 300 582
AleI CACnn'nnGTG
Cuts at: 0 865 882
Size: 865 17
AlwNI CAG_nnn'CTG
Cuts at: 0 135 594 882
Size: 135 459 288
Fragments arranged by size:
459 288 135
AvaI C'yCGr_G
Cuts at: 0 343 882
Size: 343 539
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 618 651 882
Size: 618 33 231
Fragments arranged by size:
618 231 33
BanI G'GyrC_C
Cuts at: 0 23 109 882
Size: 23 86 773
Fragments arranged by size:
773 86 23
BanII G_rGCy'C
Cuts at: 0 220 275 524 882
Size: 220 55 249 358
Fragments arranged by size:
358 249 220 55
BbeI G_GCGC'C
Cuts at: 0 113 882
Size: 113 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 668 882
Size: 668 214
BbvCI CC'TCA_GC
Cuts at: 0 725 882
Size: 725 157
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 882
Size: 646 236
BlpI GC'TnA_GC
Cuts at: 0 378 882
Size: 378 504
Bme1580I G_kGCm'C
Cuts at: 0 471 882
Size: 471 411
BmrI ACTGGGnnnn_n'
Cuts at: 0 223 750 882
Size: 223 527 132
Fragments arranged by size:
527 223 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 577 882
Size: 31 546 305
Fragments arranged by size:
546 305 31
Bpu10I CC'TnA_GC
Cuts at: 0 172 725 882
Size: 172 553 157
Fragments arranged by size:
553 172 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 882
Size: 535 347
BsaAI yAC'GTr
Cuts at: 0 301 882
Size: 301 581
BsaHI Gr'CG_yC
Cuts at: 0 110 313 882
Size: 110 203 569
Fragments arranged by size:
569 203 110
BsaWI w'CCGG_w
Cuts at: 0 578 882
Size: 578 304
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 882
Size: 301 30 551
Fragments arranged by size:
551 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 322 882
Size: 178 144 560
Fragments arranged by size:
560 178 144
BseYI C'CCAG_C
Cuts at: 0 105 551 882
Size: 105 446 331
Fragments arranged by size:
446 331 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 553 882
Size: 341 212 329
Fragments arranged by size:
341 329 212
BsiHKAI G_wGCw'C
Cuts at: 0 275 524 882
Size: 275 249 358
Fragments arranged by size:
358 275 249
BsmBI CGTCTCn'nnnn_
Cuts at: 0 304 882
Size: 304 578
Bsp1286I G_dGCh'C
Cuts at: 0 220 275 471 524 882
Size: 220 55 196 53 358
Fragments arranged by size:
358 220 196 55 53
BsrBI CCG'CTC
Cuts at: 0 15 584 882
Size: 15 569 298
Fragments arranged by size:
569 298 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 255 335 594 882
Size: 255 80 259 288
Fragments arranged by size:
288 259 255 80
BstEII G'GTnAC_C
Cuts at: 0 774 882
Size: 774 108
BtgI C'CryG_G
Cuts at: 0 605 690 882
Size: 605 85 192
Fragments arranged by size:
605 192 85
BtsI GCAGTG_nn'
Cuts at: 0 478 882
Size: 478 404
ClaI AT'CG_AT
Cuts at: 0 156 882
Size: 156 726
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 882
Size: 502 380
EaeI y'GGCC_r
Cuts at: 0 353 676 882
Size: 353 323 206
Fragments arranged by size:
353 323 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 229 882
Size: 229 653
EcoICRI GAG'CTC
Cuts at: 0 273 522 882
Size: 273 249 360
Fragments arranged by size:
360 273 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 577 882
Size: 31 546 305
Fragments arranged by size:
546 305 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 724 882
Size: 724 158
HaeII r_GCGC'y
Cuts at: 0 113 882
Size: 113 769
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 882
Size: 149 32 701
Fragments arranged by size:
701 149 32
HincII GTy'rAC
Cuts at: 0 507 882
Size: 507 375
KasI G'GCGC_C
Cuts at: 0 109 882
Size: 109 773
MscI TGG'CCA
Cuts at: 0 678 882
Size: 678 204
MslI CAynn'nnrTG
Cuts at: 0 865 882
Size: 865 17
MspA1I CmG'CkG
Cuts at: 0 132 555 671 807 882
Size: 132 423 116 136 75
Fragments arranged by size:
423 136 132 116 75
NarI GG'CG_CC
Cuts at: 0 110 882
Size: 110 772
NdeI CA'TA_TG
Cuts at: 0 864 882
Size: 864 18
PasI CC'CwG_GG
Cuts at: 0 384 882
Size: 384 498
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 882
Size: 198 684
PmlI CAC'GTG
Cuts at: 0 301 882
Size: 301 581
PspXI vC'TCGA_Gb
Cuts at: 0 343 882
Size: 343 539
PstI C_TGCA'G
Cuts at: 0 41 599 882
Size: 41 558 283
Fragments arranged by size:
558 283 41
PvuII CAG'CTG
Cuts at: 0 132 555 807 882
Size: 132 423 252 75
Fragments arranged by size:
423 252 132 75
SacI G_AGCT'C
Cuts at: 0 275 524 882
Size: 275 249 358
Fragments arranged by size:
358 275 249
SfcI C'TryA_G
Cuts at: 0 37 116 432 595 882
Size: 37 79 316 163 287
Fragments arranged by size:
316 287 163 79 37
SfoI GGC'GCC
Cuts at: 0 111 882
Size: 111 771
SmlI C'TyrA_G
Cuts at: 0 343 882
Size: 343 539
StuI AGG'CCT
Cuts at: 0 874 882
Size: 874 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 882
Size: 301 32 549
Fragments arranged by size:
549 301 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 882
Size: 102 780
XhoI C'TCGA_G
Cuts at: 0 343 882
Size: 343 539
Enzymes that do cut:
AflIII AleI AlwNI AvaI BaeI BanI BanII BbeI
BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BsrBI BstAPI BstEII BtgI BtsI
ClaI DrdI EaeI EciI EcoICRI Eco57MI EcoNI HaeII
Hin4I HincII KasI MscI MslI MspA1I NarI NdeI
PasI PflMI PmlI PspXI PstI PvuII SacI SfcI
SfoI SmlI StuI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BcgI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaBI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI CspCI
DraI DraIII EagI EarI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI Tth111I XbaI XmaI XmnI ZraI