Restriction map for
pF1KE0941
(Linear) (Six-base) MAPSORT of: KIEE0941.seq Check: 8553 from: 1 to: 804
KIEE0941.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
Acc65I G'GTAC_C
Cuts at: 0 26 804
Size: 26 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 804
Size: 460 344
AlwNI CAG_nnn'CTG
Cuts at: 0 720 804
Size: 720 84
AvaI C'yCGr_G
Cuts at: 0 167 532 804
Size: 167 365 272
Fragments arranged by size:
365 272 167
BanI G'GyrC_C
Cuts at: 0 26 804
Size: 26 778
BanII G_rGCy'C
Cuts at: 0 176 269 804
Size: 176 93 535
Fragments arranged by size:
535 176 93
BbvCI CC'TCA_GC
Cuts at: 0 272 804
Size: 272 532
BlpI GC'TnA_GC
Cuts at: 0 493 804
Size: 493 311
BmtI G_CTAG'C
Cuts at: 0 796 804
Size: 796 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 600 804
Size: 250 350 204
Fragments arranged by size:
350 250 204
Bpu10I CC'TnA_GC
Cuts at: 0 272 804
Size: 272 532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 804
Size: 709 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 269 299 573 603 804
Size: 269 30 274 30 201
Fragments arranged by size:
274 269 201 30 30
BseYI C'CCAG_C
Cuts at: 0 33 804
Size: 33 771
BsiEI CG_ry'CG
Cuts at: 0 707 804
Size: 707 97
BsiHKAI G_wGCw'C
Cuts at: 0 176 269 804
Size: 176 93 535
Fragments arranged by size:
535 176 93
BsmI GAATG_Cn'
Cuts at: 0 548 804
Size: 548 256
Bsp1286I G_dGCh'C
Cuts at: 0 176 269 804
Size: 176 93 535
Fragments arranged by size:
535 176 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 728 804
Size: 728 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 382 804
Size: 382 422
BtgI C'CryG_G
Cuts at: 0 375 787 804
Size: 375 412 17
Fragments arranged by size:
412 375 17
BtsI GCAGTG_nn'
Cuts at: 0 313 804
Size: 313 491
EaeI y'GGCC_r
Cuts at: 0 704 804
Size: 704 100
EagI C'GGCC_G
Cuts at: 0 704 804
Size: 704 100
EarI CTCTTCn'nnn_
Cuts at: 0 137 346 804
Size: 137 209 458
Fragments arranged by size:
458 209 137
EcoICRI GAG'CTC
Cuts at: 0 174 267 804
Size: 174 93 537
Fragments arranged by size:
537 174 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 460 600 804
Size: 250 210 140 204
Fragments arranged by size:
250 210 204 140
EcoO109I rG'GnC_Cy
Cuts at: 0 56 336 399 717 804
Size: 56 280 63 318 87
Fragments arranged by size:
318 280 87 63 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 804
Size: 357 447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 804
Size: 269 32 503
Fragments arranged by size:
503 269 32
KpnI G_GTAC'C
Cuts at: 0 30 804
Size: 30 774
MfeI C'AATT_G
Cuts at: 0 764 804
Size: 764 40
MslI CAynn'nnrTG
Cuts at: 0 214 380 589 804
Size: 214 166 209 215
Fragments arranged by size:
215 214 209 166
MspA1I CmG'CkG
Cuts at: 0 33 804
Size: 33 771
NcoI C'CATG_G
Cuts at: 0 375 787 804
Size: 375 412 17
Fragments arranged by size:
412 375 17
NheI G'CTAG_C
Cuts at: 0 792 804
Size: 792 12
PfoI T'CCnGG_A
Cuts at: 0 734 804
Size: 734 70
PpuMI rG'GwC_Cy
Cuts at: 0 56 399 717 804
Size: 56 343 318 87
Fragments arranged by size:
343 318 87 56
PstI C_TGCA'G
Cuts at: 0 545 804
Size: 545 259
PvuII CAG'CTG
Cuts at: 0 33 804
Size: 33 771
SacI G_AGCT'C
Cuts at: 0 176 269 804
Size: 176 93 535
Fragments arranged by size:
535 176 93
SfcI C'TryA_G
Cuts at: 0 541 804
Size: 541 263
SmlI C'TyrA_G
Cuts at: 0 724 804
Size: 724 80
StyI C'CwwG_G
Cuts at: 0 332 375 464 787 804
Size: 332 43 89 323 17
Fragments arranged by size:
332 323 89 43 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 804
Size: 577 227
TatI w'GTAC_w
Cuts at: 0 514 804
Size: 514 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 573 605 804
Size: 573 32 199
Fragments arranged by size:
573 199 32
Enzymes that do cut:
Acc65I AcuI AlwNI AvaI BanI BanII BbvCI BlpI
BmtI BpmI Bpu10I BpuEI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BstXI BtgI BtsI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I KpnI MfeI
MslI MspA1I NcoI NheI PfoI PpuMI PstI PvuII
SacI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI