Restriction map for
pF1KE0936
(Linear) (Six-base) MAPSORT of: KIEE0936.seq Check: 8655 from: 1 to: 1254
KIEE0936.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 50 266 1254
Size: 50 216 988
Fragments arranged by size:
988 216 50
Acc65I G'GTAC_C
Cuts at: 0 75 1254
Size: 75 1179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 232 1057 1254
Size: 129 103 825 197
Fragments arranged by size:
825 197 129 103
AlwNI CAG_nnn'CTG
Cuts at: 0 967 1233 1254
Size: 967 266 21
Fragments arranged by size:
967 266 21
ApaI G_GGCC'C
Cuts at: 0 491 1254
Size: 491 763
ApoI r'AATT_y
Cuts at: 0 342 1254
Size: 342 912
AvaI C'yCGr_G
Cuts at: 0 32 932 1254
Size: 32 900 322
Fragments arranged by size:
900 322 32
BanI G'GyrC_C
Cuts at: 0 75 1254
Size: 75 1179
BanII G_rGCy'C
Cuts at: 0 491 636 661 1054 1069 1190 1254
Size: 491 145 25 393 15 121 64
Fragments arranged by size:
491 393 145 121 64 25 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 426 728 1254
Size: 426 302 526
Fragments arranged by size:
526 426 302
BclI T'GATC_A
Cuts at: 0 185 1254
Size: 185 1069
BglI GCCn_nnn'nGGC
Cuts at: 0 775 1254
Size: 775 479
Bme1580I G_kGCm'C
Cuts at: 0 491 1254
Size: 491 763
BmrI ACTGGGnnnn_n'
Cuts at: 0 565 599 740 849 1254
Size: 565 34 141 109 405
Fragments arranged by size:
565 405 141 109 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 1254
Size: 820 434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 723 752 1254
Size: 348 375 29 502
Fragments arranged by size:
502 375 348 29
BsaBI GATnn'nnATC
Cuts at: 0 115 409 1254
Size: 115 294 845
Fragments arranged by size:
845 294 115
BsaHI Gr'CG_yC
Cuts at: 0 1217 1254
Size: 1217 37
BsaWI w'CCGG_w
Cuts at: 0 1148 1254
Size: 1148 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 588 618 643 673 1254
Size: 588 30 25 30 581
Fragments arranged by size:
588 581 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 644 812 852 948 1186 1219 1254
Size: 644 168 40 96 238 33 35
Fragments arranged by size:
644 238 168 96 40 35 33
BseYI C'CCAG_C
Cuts at: 0 688 1105 1254
Size: 688 417 149
Fragments arranged by size:
688 417 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1254
Size: 400 854
BsiHKAI G_wGCw'C
Cuts at: 0 661 1190 1254
Size: 661 529 64
Fragments arranged by size:
661 529 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 540 1254
Size: 540 714
Bsp1286I G_dGCh'C
Cuts at: 0 491 636 661 1054 1069 1190 1254
Size: 491 145 25 393 15 121 64
Fragments arranged by size:
491 393 145 121 64 25 15
BspHI T'CATG_A
Cuts at: 0 338 1254
Size: 338 916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 1254
Size: 168 1086
BsrBI CCG'CTC
Cuts at: 0 987 1254
Size: 987 267
BsrDI GCAATG_nn'
Cuts at: 0 323 1254
Size: 323 931
BstYI r'GATC_y
Cuts at: 0 903 1163 1254
Size: 903 260 91
Fragments arranged by size:
903 260 91
BtsI GCAGTG_nn'
Cuts at: 0 128 616 1254
Size: 128 488 638
Fragments arranged by size:
638 488 128
DraIII CAC_nnn'GTG
Cuts at: 0 1197 1254
Size: 1197 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 759 1254
Size: 759 495
EaeI y'GGCC_r
Cuts at: 0 2 90 479 674 715 830 969 1254
Size: 2 88 389 195 41 115 139 285
Fragments arranged by size:
389 285 195 139 115 88 41 2
EarI CTCTTCn'nnn_
Cuts at: 0 287 699 1254
Size: 287 412 555
Fragments arranged by size:
555 412 287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 809 1254
Size: 809 445
EcoICRI GAG'CTC
Cuts at: 0 659 1188 1254
Size: 659 529 66
Fragments arranged by size:
659 529 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 232 348 723 752 1057 1254
Size: 129 103 116 375 29 305 197
Fragments arranged by size:
375 305 197 129 116 103 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 808 1171 1254
Size: 808 363 83
Fragments arranged by size:
808 363 83
EcoO109I rG'GnC_Cy
Cuts at: 0 358 487 752 929 1254
Size: 358 129 265 177 325
Fragments arranged by size:
358 325 265 177 129
EcoRI G'AATT_C
Cuts at: 0 342 1254
Size: 342 912
HaeII r_GCGC'y
Cuts at: 0 530 1254
Size: 530 724
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1254
Size: 643 32 579
Fragments arranged by size:
643 579 32
KpnI G_GTAC'C
Cuts at: 0 79 1254
Size: 79 1175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 735 1254
Size: 135 600 519
Fragments arranged by size:
600 519 135
MscI TGG'CCA
Cuts at: 0 4 92 832 1254
Size: 4 88 740 422
Fragments arranged by size:
740 422 88 4
MspA1I CmG'CkG
Cuts at: 0 679 785 1254
Size: 679 106 469
Fragments arranged by size:
679 469 106
PasI CC'CwG_GG
Cuts at: 0 636 980 1254
Size: 636 344 274
Fragments arranged by size:
636 344 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 589 1254
Size: 589 665
PpuMI rG'GwC_Cy
Cuts at: 0 752 929 1254
Size: 752 177 325
Fragments arranged by size:
752 325 177
PspOMI G'GGCC_C
Cuts at: 0 487 1254
Size: 487 767
PstI C_TGCA'G
Cuts at: 0 522 1254
Size: 522 732
SacI G_AGCT'C
Cuts at: 0 661 1190 1254
Size: 661 529 64
Fragments arranged by size:
661 529 64
SanDI GG'GwC_CC
Cuts at: 0 929 1254
Size: 929 325
SfcI C'TryA_G
Cuts at: 0 518 1254
Size: 518 736
SmaI CCC'GGG
Cuts at: 0 34 1254
Size: 34 1220
SrfI GCCC'GGGC
Cuts at: 0 34 1254
Size: 34 1220
StuI AGG'CCT
Cuts at: 0 803 1254
Size: 803 451
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 1254
Size: 600 654
TatI w'GTAC_w
Cuts at: 0 215 1254
Size: 215 1039
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 557 589 1254
Size: 557 32 665
Fragments arranged by size:
665 557 32
XbaI T'CTAG_A
Cuts at: 0 462 1254
Size: 462 792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 669 1254
Size: 669 585
XmaI C'CCGG_G
Cuts at: 0 32 1254
Size: 32 1222
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BclI BglI Bme1580I BmrI BplI BpmI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstYI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I KpnI MmeI MscI MspA1I
PasI PflMI PpuMI PspOMI PstI SacI SanDI SfcI
SmaI SrfI StuI TaqII TatI TstI XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsiEI
BsiWI BsmI BspEI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI EagI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StyI SwaI Tth111I
XhoI XmnI ZraI