(Linear) (Six-base) MAPSORT of: KIEE0935.seq  Check: 7594  from: 1  to: 1254



KIEE0935.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AccI GT'mk_AC

Cuts at:      0      38    1254 
   Size:         38    1216
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    1254 
   Size:        289     965
 
AleI CACnn'nnGTG

Cuts at:      0     749     833    1254 
   Size:        749      84     421

  Fragments arranged by size: 

                 749     421      84
 
ApoI r'AATT_y

Cuts at:      0     278     505     656     740     824     908    1076    1160 
   Size:        278     227     151      84      84      84     168      84

Cuts at:   1160    1242    1254 
   Size:         82      12

  Fragments arranged by size: 

                 278     227     168     151      84      84      84      84
                  82      12
 
BanII G_rGCy'C

Cuts at:      0     191     970    1254 
   Size:        191     779     284

  Fragments arranged by size: 

                 779     284     191
 
BclI T'GATC_A

Cuts at:      0      75    1254 
   Size:         75    1179
 
BglII A'GATC_T

Cuts at:      0     441    1254 
   Size:        441     813
 
BmgBI CAC'GTC

Cuts at:      0     331    1254 
   Size:        331     923
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294     926    1094    1254 
   Size:        294     632     168     160

  Fragments arranged by size: 

                 632     294     168     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    1254 
   Size:        330     924
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    1254 
   Size:          5    1249
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618    1254 
   Size:        618     636
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     244    1254 
   Size:        244    1010
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     178     208    1254 
   Size:        178      30    1046

  Fragments arranged by size: 

                1046     178      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1254 
   Size:         67    1187
 
BsiHKAI G_wGCw'C

Cuts at:      0     346     970    1254 
   Size:        346     624     284

  Fragments arranged by size: 

                 624     346     284
 
BsmI GAATG_Cn'

Cuts at:      0      67     138     856     865    1254 
   Size:         67      71     718       9     389

  Fragments arranged by size: 

                 718     389      71      67       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     346     970    1254 
   Size:        191     155     624     284

  Fragments arranged by size: 

                 624     284     191     155
 
BspHI T'CATG_A

Cuts at:      0     435    1254 
   Size:        435     819
 
BsrDI GCAATG_nn'

Cuts at:      0     957    1254 
   Size:        957     297
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     860    1254 
   Size:        860     394
 
BstYI r'GATC_y

Cuts at:      0     441    1254 
   Size:        441     813
 
EaeI y'GGCC_r

Cuts at:      0     226    1254 
   Size:        226    1028
 
EcoICRI GAG'CTC

Cuts at:      0     968    1254 
   Size:        968     286
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     330    1254 
   Size:        289      41     924

  Fragments arranged by size: 

                 924     289      41
 
EcoRI G'AATT_C

Cuts at:      0     740     824     908    1076    1160    1254 
   Size:        740      84      84     168      84      94

  Fragments arranged by size: 

                 740     168      94      84      84      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     693    1254 
   Size:        693     561
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     447     479    1254 
   Size:        447      32     775

  Fragments arranged by size: 

                 775     447      32
 
MfeI C'AATT_G

Cuts at:      0     421    1254 
   Size:        421     833
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86    1254 
   Size:         86    1168
 
MscI TGG'CCA

Cuts at:      0     228    1254 
   Size:        228    1026
 
MslI CAynn'nnrTG

Cuts at:      0     749     833    1001    1169    1254 
   Size:        749      84     168     168      85

  Fragments arranged by size: 

                 749     168     168      85      84
 
PfoI T'CCnGG_A

Cuts at:      0     233    1254 
   Size:        233    1021
 
SacI G_AGCT'C

Cuts at:      0     970    1254 
   Size:        970     284
 
SexAI A'CCwGG_T

Cuts at:      0     122    1254 
   Size:        122    1132
 
SmlI C'TyrA_G

Cuts at:      0     633    1254 
   Size:        633     621
 
SpeI A'CTAG_T

Cuts at:      0    1168    1254 
   Size:       1168      86
 
StuI AGG'CCT

Cuts at:      0     871    1254 
   Size:        871     383
 
TatI w'GTAC_w

Cuts at:      0    1247    1254 
   Size:       1247       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     451     483    1254 
   Size:        451      32     771

  Fragments arranged by size: 

                 771     451      32
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApoI    BanII     BclI    BglII    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI  BsiHKAI
     BsmI Bsp1286I    BspHI    BsrDI   BstAPI    BstYI     EaeI  EcoICRI
  Eco57MI    EcoRI     FalI    Hin4I     MfeI     MmeI     MscI     MslI
     PfoI     SacI    SexAI     SmlI     SpeI     StuI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI