Restriction map for
pF1KE0932
(Linear) (Six-base) MAPSORT of: KIEE0932.seq Check: 4329 from: 1 to: 1233
KIEE0932.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 1004 1233
Size: 1004 229
AlwNI CAG_nnn'CTG
Cuts at: 0 565 1233
Size: 565 668
ApaI G_GGCC'C
Cuts at: 0 796 1233
Size: 796 437
ApaLI G'TGCA_C
Cuts at: 0 673 1233
Size: 673 560
ApoI r'AATT_y
Cuts at: 0 613 748 820 1233
Size: 613 135 72 413
Fragments arranged by size:
613 413 135 72
AvaI C'yCGr_G
Cuts at: 0 628 1233
Size: 628 605
BamHI G'GATC_C
Cuts at: 0 454 1233
Size: 454 779
BanI G'GyrC_C
Cuts at: 0 411 1233
Size: 411 822
BanII G_rGCy'C
Cuts at: 0 64 796 1233
Size: 64 732 437
Fragments arranged by size:
732 437 64
BbeI G_GCGC'C
Cuts at: 0 415 1233
Size: 415 818
BbvCI CC'TCA_GC
Cuts at: 0 333 1233
Size: 333 900
BciVI GTATCCnnnnn_n'
Cuts at: 0 1131 1233
Size: 1131 102
BclI T'GATC_A
Cuts at: 0 1040 1233
Size: 1040 193
Bme1580I G_kGCm'C
Cuts at: 0 677 796 1233
Size: 677 119 437
Fragments arranged by size:
677 437 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1233
Size: 78 1155
Bpu10I CC'TnA_GC
Cuts at: 0 333 1233
Size: 333 900
BsaI GGTCTCn'nnnn_
Cuts at: 0 635 1233
Size: 635 598
BsaHI Gr'CG_yC
Cuts at: 0 412 1233
Size: 412 821
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 132 1233
Size: 132 1101
BseYI C'CCAG_C
Cuts at: 0 372 549 859 1233
Size: 372 177 310 374
Fragments arranged by size:
374 372 310 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1233
Size: 502 731
BsiHKAI G_wGCw'C
Cuts at: 0 677 1203 1233
Size: 677 526 30
Fragments arranged by size:
677 526 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 351 961 1233
Size: 351 610 272
Fragments arranged by size:
610 351 272
Bsp1286I G_dGCh'C
Cuts at: 0 64 677 796 1203 1233
Size: 64 613 119 407 30
Fragments arranged by size:
613 407 119 64 30
BsrBI CCG'CTC
Cuts at: 0 1019 1233
Size: 1019 214
BsrDI GCAATG_nn'
Cuts at: 0 709 1233
Size: 709 524
BssSI C'ACGA_G
Cuts at: 0 260 1233
Size: 260 973
BstYI r'GATC_y
Cuts at: 0 454 1233
Size: 454 779
BstZ17I GTA'TAC
Cuts at: 0 1005 1233
Size: 1005 228
BtsI GCAGTG_nn'
Cuts at: 0 353 1233
Size: 353 880
DraI TTT'AAA
Cuts at: 0 836 1233
Size: 836 397
DraIII CAC_nnn'GTG
Cuts at: 0 112 1233
Size: 112 1121
EaeI y'GGCC_r
Cuts at: 0 323 350 424 993 1233
Size: 323 27 74 569 240
Fragments arranged by size:
569 323 240 74 27
EarI CTCTTCn'nnn_
Cuts at: 0 673 1233
Size: 673 560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1233
Size: 78 1155
EcoO109I rG'GnC_Cy
Cuts at: 0 90 306 792 793 1233
Size: 90 216 486 1 440
Fragments arranged by size:
486 440 216 90 1
HaeII r_GCGC'y
Cuts at: 0 415 1233
Size: 415 818
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 634 666 1233
Size: 526 32 76 32 567
Fragments arranged by size:
567 526 76 32 32
HindIII A'AGCT_T
Cuts at: 0 78 598 1233
Size: 78 520 635
Fragments arranged by size:
635 520 78
KasI G'GCGC_C
Cuts at: 0 411 1233
Size: 411 822
MfeI C'AATT_G
Cuts at: 0 32 1233
Size: 32 1201
MspA1I CmG'CkG
Cuts at: 0 508 1233
Size: 508 725
NarI GG'CG_CC
Cuts at: 0 412 1233
Size: 412 821
NspI r_CATG'y
Cuts at: 0 688 1233
Size: 688 545
PasI CC'CwG_GG
Cuts at: 0 406 1233
Size: 406 827
PflMI CCAn_nnn'nTGG
Cuts at: 0 112 1233
Size: 112 1121
PshAI GACnn'nnGTC
Cuts at: 0 731 1233
Size: 731 502
PspOMI G'GGCC_C
Cuts at: 0 792 1233
Size: 792 441
PstI C_TGCA'G
Cuts at: 0 24 314 1233
Size: 24 290 919
Fragments arranged by size:
919 290 24
SfcI C'TryA_G
Cuts at: 0 20 310 1233
Size: 20 290 923
Fragments arranged by size:
923 290 20
SfoI GGC'GCC
Cuts at: 0 413 1233
Size: 413 820
SmlI C'TyrA_G
Cuts at: 0 628 1233
Size: 628 605
SphI G_CATG'C
Cuts at: 0 688 1233
Size: 688 545
SspI AAT'ATT
Cuts at: 0 846 1233
Size: 846 387
StuI AGG'CCT
Cuts at: 0 490 1233
Size: 490 743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 246 278 1233
Size: 246 32 955
Fragments arranged by size:
955 246 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 1233
Size: 603 630
XhoI C'TCGA_G
Cuts at: 0 628 1233
Size: 628 605
Enzymes that do cut:
AccI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI Bme1580I BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrBI BsrDI BssSI BstYI BstZ17I BtsI DraI DraIII
EaeI EarI Eco57MI EcoO109I HaeII Hin4I HindIII KasI
MfeI MspA1I NarI NspI PasI PflMI PshAI PspOMI
PstI SfcI SfoI SmlI SphI SspI StuI TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MluI MmeI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI StyI SwaI TaqII TatI Tth111I XbaI XmaI
XmnI ZraI