(Linear) (Six-base) MAPSORT of: KIEE0931.seq  Check: 613  from: 1  to: 1170



KIEE0931.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AclI AA'CG_TT

Cuts at:      0     391     794    1170 
   Size:        391     403     376

  Fragments arranged by size: 

                 403     391     376
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     193     476     700    1043    1170 
   Size:        141      52     283     224     343     127

  Fragments arranged by size: 

                 343     283     224     141     127      52
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     189     809     822    1170 
   Size:        142      47     620      13     348

  Fragments arranged by size: 

                 620     348     142      47      13
 
AvaI C'yCGr_G

Cuts at:      0     125    1170 
   Size:        125    1045
 
BamHI G'GATC_C

Cuts at:      0     316     447     561     728    1170 
   Size:        316     131     114     167     442

  Fragments arranged by size: 

                 442     316     167     131     114
 
BanII G_rGCy'C

Cuts at:      0     716    1170 
   Size:        716     454
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     622     656    1170 
   Size:        622      34     514

  Fragments arranged by size: 

                 622     514      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    1170 
   Size:        257     913
 
Bpu10I CC'TnA_GC

Cuts at:      0     603    1170 
   Size:        603     567
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     708    1118    1170 
   Size:        361     347     410      52

  Fragments arranged by size: 

                 410     361     347      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1034    1064    1170 
   Size:       1034      30     106

  Fragments arranged by size: 

                1034     106      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      62      74    1126    1170 
   Size:         62      12    1052      44

  Fragments arranged by size: 

                1052      62      44      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     444    1170 
   Size:        444     726
 
Bsp1286I G_dGCh'C

Cuts at:      0     444     716    1170 
   Size:        444     272     454

  Fragments arranged by size: 

                 454     444     272
 
BspHI T'CATG_A

Cuts at:      0     902    1170 
   Size:        902     268
 
BstBI TT'CG_AA

Cuts at:      0     552    1170 
   Size:        552     618
 
BstYI r'GATC_y

Cuts at:      0     316     447     561     728     983    1170 
   Size:        316     131     114     167     255     187

  Fragments arranged by size: 

                 316     255     187     167     131     114
 
BtgI C'CryG_G

Cuts at:      0      18    1170 
   Size:         18    1152
 
DraIII CAC_nnn'GTG

Cuts at:      0     354     970    1170 
   Size:        354     616     200

  Fragments arranged by size: 

                 616     354     200
 
EaeI y'GGCC_r

Cuts at:      0     990    1170 
   Size:        990     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     169    1103    1170 
   Size:        169     934      67

  Fragments arranged by size: 

                 934     169      67
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     193     257     476     700    1043    1170 
   Size:        141      52      64     219     224     343     127

  Fragments arranged by size: 

                 343     224     219     141     127      64      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     991    1170 
   Size:        991     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     186    1170 
   Size:         71     115     984

  Fragments arranged by size: 

                 984     115      71
 
EcoRV GAT'ATC

Cuts at:      0     582    1170 
   Size:        582     588
 
HaeII r_GCGC'y

Cuts at:      0      36     810    1170 
   Size:         36     774     360

  Fragments arranged by size: 

                 774     360      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     243     255     275     287    1170 
   Size:        243      12      20      12     883

  Fragments arranged by size: 

                 883     243      20      12      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     639    1170 
   Size:        374     265     531

  Fragments arranged by size: 

                 531     374     265
 
MscI TGG'CCA

Cuts at:      0     992    1170 
   Size:        992     178
 
MslI CAynn'nnrTG

Cuts at:      0     901    1170 
   Size:        901     269
 
MspA1I CmG'CkG

Cuts at:      0     626    1170 
   Size:        626     544
 
NspI r_CATG'y

Cuts at:      0     622    1170 
   Size:        622     548
 
PasI CC'CwG_GG

Cuts at:      0     411    1170 
   Size:        411     759
 
PpuMI rG'GwC_Cy

Cuts at:      0     186    1170 
   Size:        186     984
 
PshAI GACnn'nnGTC

Cuts at:      0     949    1170 
   Size:        949     221
 
PstI C_TGCA'G

Cuts at:      0     831    1170 
   Size:        831     339
 
SexAI A'CCwGG_T

Cuts at:      0     362    1170 
   Size:        362     808
 
SfcI C'TryA_G

Cuts at:      0     341     512     827    1170 
   Size:        341     171     315     343

  Fragments arranged by size: 

                 343     341     315     171
 
SmaI CCC'GGG

Cuts at:      0     127    1170 
   Size:        127    1043
 
SmlI C'TyrA_G

Cuts at:      0     376     687    1133    1170 
   Size:        376     311     446      37

  Fragments arranged by size: 

                 446     376     311      37
 
StuI AGG'CCT

Cuts at:      0     166    1170 
   Size:        166    1004
 
StyI C'CwwG_G

Cuts at:      0     987    1170 
   Size:        987     183
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     558    1170 
   Size:        558     612
 
TatI w'GTAC_w

Cuts at:      0     891    1170 
   Size:        891     279
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     338     370     713     745    1170 
   Size:        338      32     343      32     425

  Fragments arranged by size: 

                 425     343     338      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     925    1170 
   Size:        925     245
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     780    1170 
   Size:        780     390
 
XmaI C'CCGG_G

Cuts at:      0     125    1170 
   Size:        125    1045
 
XmnI GAAnn'nnTTC

Cuts at:      0     302    1170 
   Size:        302     868
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     AvaI    BamHI    BanII     BcgI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BstBI
    BstYI     BtgI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NspI
     PasI    PpuMI    PshAI     PstI    SexAI     SfcI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI     ZraI