Restriction map for
pF1KE0927
(Linear) (Six-base) MAPSORT of: KIEE0927.seq Check: 606 from: 1 to: 1089
KIEE0927.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 1089
Size: 323 766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 974 1049 1089
Size: 380 594 75 40
Fragments arranged by size:
594 380 75 40
AflIII A'CryG_T
Cuts at: 0 588 1089
Size: 588 501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 1089
Size: 595 494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 61 1089
Size: 29 32 1028
Fragments arranged by size:
1028 32 29
AlwNI CAG_nnn'CTG
Cuts at: 0 408 1072 1089
Size: 408 664 17
Fragments arranged by size:
664 408 17
BamHI G'GATC_C
Cuts at: 0 58 1089
Size: 58 1031
BanI G'GyrC_C
Cuts at: 0 808 1089
Size: 808 281
BanII G_rGCy'C
Cuts at: 0 658 1089
Size: 658 431
BbsI GAAGACnn'nnnn_
Cuts at: 0 737 1089
Size: 737 352
BglII A'GATC_T
Cuts at: 0 779 1089
Size: 779 310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 672 1089
Size: 672 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1089
Size: 569 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1089
Size: 676 413
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 1089
Size: 550 539
BsaHI Gr'CG_yC
Cuts at: 0 10 1089
Size: 10 1079
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 286 316 1089
Size: 286 30 773
Fragments arranged by size:
773 286 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 973 1089
Size: 111 862 116
Fragments arranged by size:
862 116 111
BseYI C'CCAG_C
Cuts at: 0 114 705 1089
Size: 114 591 384
Fragments arranged by size:
591 384 114
BsiEI CG_ry'CG
Cuts at: 0 662 1089
Size: 662 427
BsmI GAATG_Cn'
Cuts at: 0 623 1089
Size: 623 466
Bsp1286I G_dGCh'C
Cuts at: 0 658 1089
Size: 658 431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 1043 1089
Size: 323 720 46
Fragments arranged by size:
720 323 46
BsrBI CCG'CTC
Cuts at: 0 165 620 702 891 1089
Size: 165 455 82 189 198
Fragments arranged by size:
455 198 189 165 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 347 495 543 1089
Size: 347 148 48 546
Fragments arranged by size:
546 347 148 48
BstYI r'GATC_y
Cuts at: 0 58 779 1089
Size: 58 721 310
Fragments arranged by size:
721 310 58
BtgI C'CryG_G
Cuts at: 0 527 1089
Size: 527 562
DraI TTT'AAA
Cuts at: 0 966 1089
Size: 966 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 433 1089
Size: 433 656
EaeI y'GGCC_r
Cuts at: 0 185 320 659 986 1089
Size: 185 135 339 327 103
Fragments arranged by size:
339 327 185 135 103
EagI C'GGCC_G
Cuts at: 0 659 1089
Size: 659 430
EarI CTCTTCn'nnn_
Cuts at: 0 610 950 1089
Size: 610 340 139
Fragments arranged by size:
610 340 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 569 974 1049 1089
Size: 380 189 405 75 40
Fragments arranged by size:
405 380 189 75 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 412 1089
Size: 412 677
EcoO109I rG'GnC_Cy
Cuts at: 0 366 405 738 1089
Size: 366 39 333 351
Fragments arranged by size:
366 351 333 39
MscI TGG'CCA
Cuts at: 0 187 322 1089
Size: 187 135 767
Fragments arranged by size:
767 187 135
MslI CAynn'nnrTG
Cuts at: 0 151 1089
Size: 151 938
NspI r_CATG'y
Cuts at: 0 46 207 465 592 771 835 1089
Size: 46 161 258 127 179 64 254
Fragments arranged by size:
258 254 179 161 127 64 46
PciI A'CATG_T
Cuts at: 0 588 1089
Size: 588 501
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 1089
Size: 726 363
PpuMI rG'GwC_Cy
Cuts at: 0 366 1089
Size: 366 723
PsiI TTA'TAA
Cuts at: 0 746 1089
Size: 746 343
PstI C_TGCA'G
Cuts at: 0 413 1077 1089
Size: 413 664 12
Fragments arranged by size:
664 413 12
SapI GCTCTTCn'nnn_
Cuts at: 0 610 1089
Size: 610 479
SbfI CC_TGCA'GG
Cuts at: 0 413 1089
Size: 413 676
SfcI C'TryA_G
Cuts at: 0 409 1073 1089
Size: 409 664 16
Fragments arranged by size:
664 409 16
SmlI C'TyrA_G
Cuts at: 0 691 1089
Size: 691 398
SphI G_CATG'C
Cuts at: 0 465 835 1089
Size: 465 370 254
Fragments arranged by size:
465 370 254
StuI AGG'CCT
Cuts at: 0 598 1089
Size: 598 491
StyI C'CwwG_G
Cuts at: 0 488 1089
Size: 488 601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 330 939 1089
Size: 279 51 609 150
Fragments arranged by size:
609 279 150 51
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI BamHI BanI
BanII BbsI BglII BplI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsiEI BsmI Bsp1286I BspMI BsrBI
BstXI BstYI BtgI DraI DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I MscI MslI NspI PciI PflMI
PpuMI PsiI PstI SapI SbfI SfcI SmlI SphI
StuI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaWI BsgI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI