Restriction map for
pF1KE0926
(Linear) (Six-base) MAPSORT of: KIEE0926.seq Check: 1099 from: 1 to: 1077
KIEE0926.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 565 1077
Size: 565 512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 544 1077
Size: 377 167 533
Fragments arranged by size:
533 377 167
AflIII A'CryG_T
Cuts at: 0 471 1077
Size: 471 606
AhdI GACnn_n'nnGTC
Cuts at: 0 1044 1077
Size: 1044 33
AleI CACnn'nnGTG
Cuts at: 0 824 1077
Size: 824 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 101 1077
Size: 101 976
AlwNI CAG_nnn'CTG
Cuts at: 0 427 552 738 1077
Size: 427 125 186 339
Fragments arranged by size:
427 339 186 125
ApaI G_GGCC'C
Cuts at: 0 592 1077
Size: 592 485
ApaLI G'TGCA_C
Cuts at: 0 1005 1077
Size: 1005 72
ApoI r'AATT_y
Cuts at: 0 680 940 1077
Size: 680 260 137
Fragments arranged by size:
680 260 137
BamHI G'GATC_C
Cuts at: 0 718 1077
Size: 718 359
BanII G_rGCy'C
Cuts at: 0 209 592 962 1077
Size: 209 383 370 115
Fragments arranged by size:
383 370 209 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 532 816 1077
Size: 335 197 284 261
Fragments arranged by size:
335 284 261 197
BbvCI CC'TCA_GC
Cuts at: 0 723 1077
Size: 723 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1042 1076 1077
Size: 1042 34 1
Fragments arranged by size:
1042 34 1
BglII A'GATC_T
Cuts at: 0 464 1077
Size: 464 613
Bme1580I G_kGCm'C
Cuts at: 0 592 1009 1077
Size: 592 417 68
Fragments arranged by size:
592 417 68
BmgBI CAC'GTC
Cuts at: 0 371 1077
Size: 371 706
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 1077
Size: 529 548
BmtI G_CTAG'C
Cuts at: 0 305 1077
Size: 305 772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1077
Size: 277 800
Bpu10I CC'TnA_GC
Cuts at: 0 723 1077
Size: 723 354
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 646 676 1077
Size: 646 30 401
Fragments arranged by size:
646 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 278 429 635 712 1077
Size: 278 151 206 77 365
Fragments arranged by size:
365 278 206 151 77
BseYI C'CCAG_C
Cuts at: 0 177 1077
Size: 177 900
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1077
Size: 565 512
BsiHKAI G_wGCw'C
Cuts at: 0 391 451 962 1009 1077
Size: 391 60 511 47 68
Fragments arranged by size:
511 391 68 60 47
Bsp1286I G_dGCh'C
Cuts at: 0 209 391 451 592 962 1009 1077
Size: 209 182 60 141 370 47 68
Fragments arranged by size:
370 209 182 141 68 60 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 57 69 1077
Size: 57 12 1008
Fragments arranged by size:
1008 57 12
BsrDI GCAATG_nn'
Cuts at: 0 773 1077
Size: 773 304
BsrGI T'GTAC_A
Cuts at: 0 851 1077
Size: 851 226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 738 1013 1077
Size: 552 186 275 64
Fragments arranged by size:
552 275 186 64
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 537 1077
Size: 168 369 540
Fragments arranged by size:
540 369 168
BstYI r'GATC_y
Cuts at: 0 105 464 718 1077
Size: 105 359 254 359
Fragments arranged by size:
359 359 254 105
BstZ17I GTA'TAC
Cuts at: 0 566 1077
Size: 566 511
BtgI C'CryG_G
Cuts at: 0 167 557 1077
Size: 167 390 520
Fragments arranged by size:
520 390 167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1039 1077
Size: 1039 38
BtsI GCAGTG_nn'
Cuts at: 0 83 1077
Size: 83 994
EarI CTCTTCn'nnn_
Cuts at: 0 782 799 1077
Size: 782 17 278
Fragments arranged by size:
782 278 17
EcoICRI GAG'CTC
Cuts at: 0 960 1077
Size: 960 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 377 544 1077
Size: 277 100 167 533
Fragments arranged by size:
533 277 167 100
EcoO109I rG'GnC_Cy
Cuts at: 0 588 589 1077
Size: 588 1 488
Fragments arranged by size:
588 488 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 811 1077
Size: 811 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 646 678 703 735 916 948
Size: 426 32 188 32 25 32 181 32
Cuts at: 948 1030 1062 1077
Size: 82 32 15
Fragments arranged by size:
426 188 181 82 32 32 32 32
32 25 15
HincII GTy'rAC
Cuts at: 0 261 374 654 1077
Size: 261 113 280 423
Fragments arranged by size:
423 280 261 113
MfeI C'AATT_G
Cuts at: 0 707 1077
Size: 707 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 169 1077
Size: 63 106 908
Fragments arranged by size:
908 106 63
MslI CAynn'nnrTG
Cuts at: 0 535 664 824 1077
Size: 535 129 160 253
Fragments arranged by size:
535 253 160 129
NcoI C'CATG_G
Cuts at: 0 167 557 1077
Size: 167 390 520
Fragments arranged by size:
520 390 167
NheI G'CTAG_C
Cuts at: 0 301 1077
Size: 301 776
NspI r_CATG'y
Cuts at: 0 309 475 1077
Size: 309 166 602
Fragments arranged by size:
602 309 166
PciI A'CATG_T
Cuts at: 0 471 1077
Size: 471 606
PflMI CCAn_nnn'nTGG
Cuts at: 0 616 1077
Size: 616 461
PfoI T'CCnGG_A
Cuts at: 0 152 934 1077
Size: 152 782 143
Fragments arranged by size:
782 152 143
PsiI TTA'TAA
Cuts at: 0 911 1077
Size: 911 166
PspOMI G'GGCC_C
Cuts at: 0 588 1077
Size: 588 489
PstI C_TGCA'G
Cuts at: 0 582 597 743 1077
Size: 582 15 146 334
Fragments arranged by size:
582 334 146 15
SacI G_AGCT'C
Cuts at: 0 962 1077
Size: 962 115
SfcI C'TryA_G
Cuts at: 0 578 593 739 748 1077
Size: 578 15 146 9 329
Fragments arranged by size:
578 329 146 15 9
SphI G_CATG'C
Cuts at: 0 309 1077
Size: 309 768
StyI C'CwwG_G
Cuts at: 0 167 281 557 1077
Size: 167 114 276 520
Fragments arranged by size:
520 276 167 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 505 1077
Size: 505 572
TatI w'GTAC_w
Cuts at: 0 703 851 1077
Size: 703 148 226
Fragments arranged by size:
703 226 148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 1077
Size: 672 405
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI BamHI BanII BbsI BbvCI BcgI BglII
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstAPI
BstXI BstYI BstZ17I BtgI BtgZI BtsI EarI EcoICRI
Eco57MI EcoO109I FalI Hin4I HincII MfeI MmeI MslI
NcoI NheI NspI PciI PflMI PfoI PsiI PspOMI
PstI SacI SfcI SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BciVI BclI BfrBI BglI BlpI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII
Bsu36I ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI