(Linear) (Six-base) MAPSORT of: KIEE0925.seq  Check: 2082  from: 1  to: 984



KIEE0925.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     199     984 
   Size:        199     785
 
ApoI r'AATT_y

Cuts at:      0     123     346     448     644     984 
   Size:        123     223     102     196     340

  Fragments arranged by size: 

                 340     223     196     123     102
 
AseI AT'TA_AT

Cuts at:      0     251     984 
   Size:        251     733
 
AvaI C'yCGr_G

Cuts at:      0      43     984 
   Size:         43     941
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     613     646     984 
   Size:        613      33     338

  Fragments arranged by size: 

                 613     338      33
 
BanI G'GyrC_C

Cuts at:      0      23     984 
   Size:         23     961
 
BanII G_rGCy'C

Cuts at:      0      62      90     984 
   Size:         62      28     894

  Fragments arranged by size: 

                 894      62      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     466     984 
   Size:         42     424     518

  Fragments arranged by size: 

                 518     424      42
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     614     735     984 
   Size:        614     121     249

  Fragments arranged by size: 

                 614     249     121
 
BlpI GC'TnA_GC

Cuts at:      0     239     984 
   Size:        239     745
 
Bpu10I CC'TnA_GC

Cuts at:      0     971     984 
   Size:        971      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     984 
   Size:        254     730
 
BsaBI GATnn'nnATC

Cuts at:      0     746     984 
   Size:        746     238
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      77     107     984 
   Size:         77      30     877

  Fragments arranged by size: 

                 877      77      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      98     984 
   Size:         98     886
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     984 
   Size:        614     370
 
BsiHKAI G_wGCw'C

Cuts at:      0      62     984 
   Size:         62     922
 
Bsp1286I G_dGCh'C

Cuts at:      0      62      90     984 
   Size:         62      28     894

  Fragments arranged by size: 

                 894      62      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     234     984 
   Size:        234     750
 
BsrDI GCAATG_nn'

Cuts at:      0      15     984 
   Size:         15     969
 
BtgI C'CryG_G

Cuts at:      0     737     984 
   Size:        737     247
 
DraI TTT'AAA

Cuts at:      0     342     984 
   Size:        342     642
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      46     984 
   Size:         46     938
 
EaeI y'GGCC_r

Cuts at:      0     662     984 
   Size:        662     322
 
EcoICRI GAG'CTC

Cuts at:      0      60     984 
   Size:         60     924
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     984 
   Size:        316      32     636

  Fragments arranged by size: 

                 636     316      32
 
MscI TGG'CCA

Cuts at:      0     664     984 
   Size:        664     320
 
MslI CAynn'nnrTG

Cuts at:      0     308     984 
   Size:        308     676
 
NcoI C'CATG_G

Cuts at:      0     737     984 
   Size:        737     247
 
NspI r_CATG'y

Cuts at:      0     572     984 
   Size:        572     412
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     148     984 
   Size:        148     836
 
SacI G_AGCT'C

Cuts at:      0      62     984 
   Size:         62     922
 
SmaI CCC'GGG

Cuts at:      0      45     984 
   Size:         45     939
 
SmlI C'TyrA_G

Cuts at:      0     269     984 
   Size:        269     715
 
SpeI A'CTAG_T

Cuts at:      0     930     984 
   Size:        930      54
 
SphI G_CATG'C

Cuts at:      0     572     984 
   Size:        572     412
 
StuI AGG'CCT

Cuts at:      0     603     984 
   Size:        603     381
 
StyI C'CwwG_G

Cuts at:      0     737     984 
   Size:        737     247
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     567     984 
   Size:        567     417
 
TatI w'GTAC_w

Cuts at:      0     414     629     819     984 
   Size:        414     215     190     165

  Fragments arranged by size: 

                 414     215     190     165
 
XmaI C'CCGG_G

Cuts at:      0      43     984 
   Size:         43     941
 

 Enzymes that do cut:

    AlwNI     ApoI     AseI     AvaI     BaeI     BanI    BanII     BbsI
    BciVI     BlpI   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI     BtgI     DraI     DrdI     EaeI
  EcoICRI    Hin4I     MscI     MslI     NcoI     NspI    PflMI     SacI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI    AsiSI    AvrII    BamHI     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI