Restriction map for
pF1KE0920
(Linear) (Six-base) MAPSORT of: KIEE0920.seq Check: 5581 from: 1 to: 939
KIEE0920.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 41 438 939
Size: 41 397 501
Fragments arranged by size:
501 397 41
AccI GT'mk_AC
Cuts at: 0 155 590 939
Size: 155 435 349
Fragments arranged by size:
435 349 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 747 764 939
Size: 298 449 17 175
Fragments arranged by size:
449 298 175 17
AflIII A'CryG_T
Cuts at: 0 201 939
Size: 201 738
AhdI GACnn_n'nnGTC
Cuts at: 0 561 939
Size: 561 378
AleI CACnn'nnGTG
Cuts at: 0 253 939
Size: 253 686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 271 939
Size: 73 198 668
Fragments arranged by size:
668 198 73
AlwNI CAG_nnn'CTG
Cuts at: 0 453 939
Size: 453 486
ApaI G_GGCC'C
Cuts at: 0 124 932 939
Size: 124 808 7
Fragments arranged by size:
808 124 7
AvaI C'yCGr_G
Cuts at: 0 251 291 367 611 939
Size: 251 40 76 244 328
Fragments arranged by size:
328 251 244 76 40
BamHI G'GATC_C
Cuts at: 0 635 686 939
Size: 635 51 253
Fragments arranged by size:
635 253 51
BanI G'GyrC_C
Cuts at: 0 75 939
Size: 75 864
BanII G_rGCy'C
Cuts at: 0 98 124 292 414 545 932 939
Size: 98 26 168 122 131 387 7
Fragments arranged by size:
387 168 131 122 98 26 7
BbeI G_GCGC'C
Cuts at: 0 79 939
Size: 79 860
BglII A'GATC_T
Cuts at: 0 381 939
Size: 381 558
Bme1580I G_kGCm'C
Cuts at: 0 124 260 932 939
Size: 124 136 672 7
Fragments arranged by size:
672 136 124 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 237 939
Size: 139 98 702
Fragments arranged by size:
702 139 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 379 475 611 939
Size: 121 258 96 136 328
Fragments arranged by size:
328 258 136 121 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 562 939
Size: 529 33 377
Fragments arranged by size:
529 377 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 780 813 939
Size: 780 33 126
Fragments arranged by size:
780 126 33
BsaAI yAC'GTr
Cuts at: 0 204 939
Size: 204 735
BsaHI Gr'CG_yC
Cuts at: 0 76 305 939
Size: 76 229 634
Fragments arranged by size:
634 229 76
BsaWI w'CCGG_w
Cuts at: 0 215 939
Size: 215 724
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 939
Size: 94 30 815
Fragments arranged by size:
815 94 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 939
Size: 436 503
BseYI C'CCAG_C
Cuts at: 0 931 939
Size: 931 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 939
Size: 43 896
BsiHKAI G_wGCw'C
Cuts at: 0 292 491 545 939
Size: 292 199 54 394
Fragments arranged by size:
394 292 199 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 177 939
Size: 177 762
Bsp1286I G_dGCh'C
Cuts at: 0 98 124 260 292 414 491 545 932
Size: 98 26 136 32 122 77 54 387
Cuts at: 932 939
Size: 7
Fragments arranged by size:
387 136 122 98 77 54 32 26
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 65 438 650 939
Size: 41 24 373 212 289
Fragments arranged by size:
373 289 212 41 24
BsrFI r'CCGG_y
Cuts at: 0 186 372 939
Size: 186 186 567
Fragments arranged by size:
567 186 186
BssHII G'CGCG_C
Cuts at: 0 36 939
Size: 36 903
BstBI TT'CG_AA
Cuts at: 0 842 939
Size: 842 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 267 939
Size: 72 195 672
Fragments arranged by size:
672 195 72
BstYI r'GATC_y
Cuts at: 0 381 635 686 902 939
Size: 381 254 51 216 37
Fragments arranged by size:
381 254 216 51 37
BtgI C'CryG_G
Cuts at: 0 260 654 862 939
Size: 260 394 208 77
Fragments arranged by size:
394 260 208 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 631 939
Size: 631 308
BtsI GCAGTG_nn'
Cuts at: 0 468 939
Size: 468 471
DrdI GACnn_nn'nnGTC
Cuts at: 0 346 939
Size: 346 593
EaeI y'GGCC_r
Cuts at: 0 191 939
Size: 191 748
EarI CTCTTCn'nnn_
Cuts at: 0 391 731 783 939
Size: 391 340 52 156
Fragments arranged by size:
391 340 156 52
EcoICRI GAG'CTC
Cuts at: 0 290 543 939
Size: 290 253 396
Fragments arranged by size:
396 290 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 298 379 475 611 747 764 939
Size: 121 177 81 96 136 136 17 175
Fragments arranged by size:
177 175 136 136 121 96 81 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 886 939
Size: 418 468 53
Fragments arranged by size:
468 418 53
EcoO109I rG'GnC_Cy
Cuts at: 0 608 928 939
Size: 608 320 11
Fragments arranged by size:
608 320 11
HaeII r_GCGC'y
Cuts at: 0 79 574 939
Size: 79 495 365
Fragments arranged by size:
495 365 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 939
Size: 547 32 360
Fragments arranged by size:
547 360 32
KasI G'GCGC_C
Cuts at: 0 75 939
Size: 75 864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 248 939
Size: 200 48 691
Fragments arranged by size:
691 200 48
MscI TGG'CCA
Cuts at: 0 193 939
Size: 193 746
MslI CAynn'nnrTG
Cuts at: 0 253 265 939
Size: 253 12 674
Fragments arranged by size:
674 253 12
MspA1I CmG'CkG
Cuts at: 0 137 939
Size: 137 802
NarI GG'CG_CC
Cuts at: 0 76 939
Size: 76 863
NcoI C'CATG_G
Cuts at: 0 260 654 862 939
Size: 260 394 208 77
Fragments arranged by size:
394 260 208 77
PasI CC'CwG_GG
Cuts at: 0 681 939
Size: 681 258
PfoI T'CCnGG_A
Cuts at: 0 317 356 939
Size: 317 39 583
Fragments arranged by size:
583 317 39
PmlI CAC'GTG
Cuts at: 0 204 939
Size: 204 735
PpuMI rG'GwC_Cy
Cuts at: 0 608 939
Size: 608 331
PspOMI G'GGCC_C
Cuts at: 0 120 928 939
Size: 120 808 11
Fragments arranged by size:
808 120 11
PspXI vC'TCGA_Gb
Cuts at: 0 251 939
Size: 251 688
SacI G_AGCT'C
Cuts at: 0 292 545 939
Size: 292 253 394
Fragments arranged by size:
394 292 253
SanDI GG'GwC_CC
Cuts at: 0 608 939
Size: 608 331
SexAI A'CCwGG_T
Cuts at: 0 113 939
Size: 113 826
SfoI GGC'GCC
Cuts at: 0 77 939
Size: 77 862
SmlI C'TyrA_G
Cuts at: 0 251 544 577 939
Size: 251 293 33 362
Fragments arranged by size:
362 293 251 33
StyI C'CwwG_G
Cuts at: 0 260 654 862 939
Size: 260 394 208 77
Fragments arranged by size:
394 260 208 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 939
Size: 141 798
Tth111I GACn'n_nGTC
Cuts at: 0 586 939
Size: 586 353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 201 652 939
Size: 201 451 287
Fragments arranged by size:
451 287 201
XhoI C'TCGA_G
Cuts at: 0 251 939
Size: 251 688
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BglII Bme1580I
BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssHII BstBI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI PasI
PfoI PmlI PpuMI PspOMI PspXI SacI SanDI SexAI
SfoI SmlI StyI TaqII Tth111I XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI
BplI Bpu10I BsaBI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XmaI XmnI ZraI