Restriction map for
pF1KE0915
(Linear) (Six-base) MAPSORT of: KIEE0915.seq Check: 2130 from: 1 to: 789
KIEE0915.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AgeI A'CCGG_T
Cuts at: 0 33 789
Size: 33 756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 345 789
Size: 345 444
AlwNI CAG_nnn'CTG
Cuts at: 0 338 789
Size: 338 451
BamHI G'GATC_C
Cuts at: 0 728 789
Size: 728 61
BanI G'GyrC_C
Cuts at: 0 304 469 789
Size: 304 165 320
Fragments arranged by size:
320 304 165
BfrBI ATG'CAT
Cuts at: 0 580 789
Size: 580 209
BglII A'GATC_T
Cuts at: 0 75 789
Size: 75 714
Bme1580I G_kGCm'C
Cuts at: 0 472 789
Size: 472 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 723 789
Size: 213 510 66
Fragments arranged by size:
510 213 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 789
Size: 18 771
BsaAI yAC'GTr
Cuts at: 0 516 711 789
Size: 516 195 78
Fragments arranged by size:
516 195 78
BsaBI GATnn'nnATC
Cuts at: 0 283 789
Size: 283 506
BsaWI w'CCGG_w
Cuts at: 0 33 533 789
Size: 33 500 256
Fragments arranged by size:
500 256 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 200 230 241 271 680 710
Size: 90 30 80 30 11 30 409 30
Cuts at: 710 789
Size: 79
Fragments arranged by size:
409 90 80 79 30 30 30 30
11
BseYI C'CCAG_C
Cuts at: 0 134 313 485 789
Size: 134 179 172 304
Fragments arranged by size:
304 179 172 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 713 789
Size: 92 621 76
Fragments arranged by size:
621 92 76
Bsp1286I G_dGCh'C
Cuts at: 0 472 789
Size: 472 317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 789
Size: 305 484
BsrDI GCAATG_nn'
Cuts at: 0 251 367 789
Size: 251 116 422
Fragments arranged by size:
422 251 116
BsrFI r'CCGG_y
Cuts at: 0 33 789
Size: 33 756
BstBI TT'CG_AA
Cuts at: 0 381 789
Size: 381 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 789
Size: 18 771
BstYI r'GATC_y
Cuts at: 0 75 84 728 789
Size: 75 9 644 61
Fragments arranged by size:
644 75 61 9
BtgI C'CryG_G
Cuts at: 0 204 308 464 789
Size: 204 104 156 325
Fragments arranged by size:
325 204 156 104
DrdI GACnn_nn'nnGTC
Cuts at: 0 65 789
Size: 65 724
EaeI y'GGCC_r
Cuts at: 0 122 789
Size: 122 667
EarI CTCTTCn'nnn_
Cuts at: 0 143 789
Size: 143 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 190 789
Size: 190 599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 723 789
Size: 213 510 66
Fragments arranged by size:
510 213 66
EcoO109I rG'GnC_Cy
Cuts at: 0 499 789
Size: 499 290
HaeII r_GCGC'y
Cuts at: 0 7 789
Size: 7 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 667 680 699 712 789
Size: 667 13 19 13 77
Fragments arranged by size:
667 77 19 13 13
HincII GTy'rAC
Cuts at: 0 699 789
Size: 699 90
MspA1I CmG'CkG
Cuts at: 0 223 241 325 409 789
Size: 223 18 84 84 380
Fragments arranged by size:
380 223 84 84 18
NcoI C'CATG_G
Cuts at: 0 204 308 464 789
Size: 204 104 156 325
Fragments arranged by size:
325 204 156 104
NdeI CA'TA_TG
Cuts at: 0 582 789
Size: 582 207
NsiI A_TGCA'T
Cuts at: 0 582 789
Size: 582 207
PmlI CAC'GTG
Cuts at: 0 711 789
Size: 711 78
PvuII CAG'CTG
Cuts at: 0 223 409 789
Size: 223 186 380
Fragments arranged by size:
380 223 186
SapI GCTCTTCn'nnn_
Cuts at: 0 143 789
Size: 143 646
SfcI C'TryA_G
Cuts at: 0 236 401 557 669 789
Size: 236 165 156 112 120
Fragments arranged by size:
236 165 156 120 112
StuI AGG'CCT
Cuts at: 0 631 789
Size: 631 158
StyI C'CwwG_G
Cuts at: 0 204 308 464 789
Size: 204 104 156 325
Fragments arranged by size:
325 204 156 104
TatI w'GTAC_w
Cuts at: 0 443 789
Size: 443 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 789
Size: 90 32 667
Fragments arranged by size:
667 90 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 747 789
Size: 747 42
XmnI GAAnn'nnTTC
Cuts at: 0 167 789
Size: 167 622
Enzymes that do cut:
AgeI AlfI AlwNI BamHI BanI BfrBI BglII Bme1580I
BpmI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
Bsp1286I BspMI BsrDI BsrFI BstBI BstXI BstYI BtgI
DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
HincII MspA1I NcoI NdeI NsiI PmlI PvuII SapI
SfcI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI