Restriction map for
pF1KE0911
(Linear) (Six-base) MAPSORT of: KIEE0911.seq Check: 7131 from: 1 to: 717
KIEE0911.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 224 448 717
Size: 224 224 269
Fragments arranged by size:
269 224 224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 717
Size: 665 52
ApaI G_GGCC'C
Cuts at: 0 165 717
Size: 165 552
ApoI r'AATT_y
Cuts at: 0 670 678 717
Size: 670 8 39
Fragments arranged by size:
670 39 8
BamHI G'GATC_C
Cuts at: 0 488 717
Size: 488 229
BanII G_rGCy'C
Cuts at: 0 165 539 717
Size: 165 374 178
Fragments arranged by size:
374 178 165
BglI GCCn_nnn'nGGC
Cuts at: 0 326 717
Size: 326 391
Bme1580I G_kGCm'C
Cuts at: 0 62 165 717
Size: 62 103 552
Fragments arranged by size:
552 103 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 67 151 717
Size: 52 15 84 566
Fragments arranged by size:
566 84 52 15
BsaAI yAC'GTr
Cuts at: 0 236 405 717
Size: 236 169 312
Fragments arranged by size:
312 236 169
BsaHI Gr'CG_yC
Cuts at: 0 200 717
Size: 200 517
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 717
Size: 268 449
BseYI C'CCAG_C
Cuts at: 0 324 717
Size: 324 393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 133 138 613 717
Size: 52 81 5 475 104
Fragments arranged by size:
475 104 81 52 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 207 717
Size: 207 510
Bsp1286I G_dGCh'C
Cuts at: 0 62 165 539 717
Size: 62 103 374 178
Fragments arranged by size:
374 178 103 62
BspHI T'CATG_A
Cuts at: 0 17 717
Size: 17 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 448 717
Size: 224 224 269
Fragments arranged by size:
269 224 224
BsrDI GCAATG_nn'
Cuts at: 0 365 717
Size: 365 352
BsrFI r'CCGG_y
Cuts at: 0 196 320 717
Size: 196 124 397
Fragments arranged by size:
397 196 124
BssHII G'CGCG_C
Cuts at: 0 192 717
Size: 192 525
BssSI C'ACGA_G
Cuts at: 0 70 717
Size: 70 647
BstAPI GCAn_nnn'nTGC
Cuts at: 0 440 717
Size: 440 277
BstYI r'GATC_y
Cuts at: 0 488 717
Size: 488 229
BtsI GCAGTG_nn'
Cuts at: 0 150 717
Size: 150 567
DraIII CAC_nnn'GTG
Cuts at: 0 75 591 717
Size: 75 516 126
Fragments arranged by size:
516 126 75
DrdI GACnn_nn'nnGTC
Cuts at: 0 301 717
Size: 301 416
EaeI y'GGCC_r
Cuts at: 0 174 717
Size: 174 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 67 151 665 717
Size: 52 15 84 514 52
Fragments arranged by size:
514 84 52 52 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 106 717
Size: 106 611
EcoO109I rG'GnC_Cy
Cuts at: 0 161 162 717
Size: 161 1 555
Fragments arranged by size:
555 161 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 717
Size: 499 32 186
Fragments arranged by size:
499 186 32
MspA1I CmG'CkG
Cuts at: 0 147 180 717
Size: 147 33 537
Fragments arranged by size:
537 147 33
NaeI GCC'GGC
Cuts at: 0 198 322 717
Size: 198 124 395
Fragments arranged by size:
395 198 124
NgoMIV G'CCGG_C
Cuts at: 0 196 320 717
Size: 196 124 397
Fragments arranged by size:
397 196 124
PasI CC'CwG_GG
Cuts at: 0 6 717
Size: 6 711
PfoI T'CCnGG_A
Cuts at: 0 128 375 717
Size: 128 247 342
Fragments arranged by size:
342 247 128
PmlI CAC'GTG
Cuts at: 0 236 405 717
Size: 236 169 312
Fragments arranged by size:
312 236 169
PspOMI G'GGCC_C
Cuts at: 0 161 717
Size: 161 556
PvuII CAG'CTG
Cuts at: 0 147 180 717
Size: 147 33 537
Fragments arranged by size:
537 147 33
SgrAI Cr'CCGG_yG
Cuts at: 0 196 717
Size: 196 521
TatI w'GTAC_w
Cuts at: 0 50 717
Size: 50 667
Tth111I GACn'n_nGTC
Cuts at: 0 124 717
Size: 124 593
XmnI GAAnn'nnTTC
Cuts at: 0 517 717
Size: 517 200
Enzymes that do cut:
AarI AcuI ApaI ApoI BamHI BanII BglI Bme1580I
BpmI BsaAI BsaHI BseRI BseYI BsgI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstYI
BtsI DraIII DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I
MspA1I NaeI NgoMIV PasI PfoI PmlI PspOMI PvuII
SgrAI TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI BsrGI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI